Investigating the influence of physiologically relevant hydrostatic pressure on CHO cell batch culture

Abstract Chinese hamster ovary (CHO) cells have been the most commonly used mammalian host for large-scale commercial production of therapeutic proteins, such as monoclonal antibodies. Enhancement of productivity of these CHO cells is one of the top priorities in the biopharmaceutical industry to re...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Menglin Shang, Taehong Kwon, Jean-Francois P. Hamel, Chwee Teck Lim, Bee Luan Khoo, Jongyoon Han
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/da12497fc0964278b9a19b664192776e
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:da12497fc0964278b9a19b664192776e
record_format	dspace
spelling	oai:doaj.org-article:da12497fc0964278b9a19b664192776e2021-12-02T11:46:01ZInvestigating the influence of physiologically relevant hydrostatic pressure on CHO cell batch culture10.1038/s41598-020-80576-82045-2322https://doaj.org/article/da12497fc0964278b9a19b664192776e2021-01-01T00:00:00Zhttps://doi.org/10.1038/s41598-020-80576-8https://doaj.org/toc/2045-2322Abstract Chinese hamster ovary (CHO) cells have been the most commonly used mammalian host for large-scale commercial production of therapeutic proteins, such as monoclonal antibodies. Enhancement of productivity of these CHO cells is one of the top priorities in the biopharmaceutical industry to reduce manufacturing cost. Although there are many different methods (e.g. temperature, pH, feed) to improve protein production in CHO cells, the role of physiologically relevant hydrostatic pressure in CHO cell culture has not been reported yet. In this study, four different hydrostatic pressures (0, 30, 60, and 90 mmHg) were applied to batch CHO cells, and their cell growth/metabolism and IgG1 production were examined. Our results indicate that hydrostatic pressure can increase the maximum cell concentration by up to 50%. Moreover, overall IgG1 concentration on Day 5 showed that 30 mmHg pressure can increase IgG1 production by 26%. The percentage of non-disulphide-linked antibody aggregates had no significant change under pressure. Besides, no significant difference was observed between 30 mmHg and no pressure conditions in terms of cell clumping formation. All these findings are important for the optimization of fed-batch or perfusion culture for directing cell growth and improving antibody production.Menglin ShangTaehong KwonJean-Francois P. HamelChwee Teck LimBee Luan KhooJongyoon HanNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-9 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Menglin Shang Taehong Kwon Jean-Francois P. Hamel Chwee Teck Lim Bee Luan Khoo Jongyoon Han Investigating the influence of physiologically relevant hydrostatic pressure on CHO cell batch culture
description	Abstract Chinese hamster ovary (CHO) cells have been the most commonly used mammalian host for large-scale commercial production of therapeutic proteins, such as monoclonal antibodies. Enhancement of productivity of these CHO cells is one of the top priorities in the biopharmaceutical industry to reduce manufacturing cost. Although there are many different methods (e.g. temperature, pH, feed) to improve protein production in CHO cells, the role of physiologically relevant hydrostatic pressure in CHO cell culture has not been reported yet. In this study, four different hydrostatic pressures (0, 30, 60, and 90 mmHg) were applied to batch CHO cells, and their cell growth/metabolism and IgG1 production were examined. Our results indicate that hydrostatic pressure can increase the maximum cell concentration by up to 50%. Moreover, overall IgG1 concentration on Day 5 showed that 30 mmHg pressure can increase IgG1 production by 26%. The percentage of non-disulphide-linked antibody aggregates had no significant change under pressure. Besides, no significant difference was observed between 30 mmHg and no pressure conditions in terms of cell clumping formation. All these findings are important for the optimization of fed-batch or perfusion culture for directing cell growth and improving antibody production.
format	article
author	Menglin Shang Taehong Kwon Jean-Francois P. Hamel Chwee Teck Lim Bee Luan Khoo Jongyoon Han
author_facet	Menglin Shang Taehong Kwon Jean-Francois P. Hamel Chwee Teck Lim Bee Luan Khoo Jongyoon Han
author_sort	Menglin Shang
title	Investigating the influence of physiologically relevant hydrostatic pressure on CHO cell batch culture
title_short	Investigating the influence of physiologically relevant hydrostatic pressure on CHO cell batch culture
title_full	Investigating the influence of physiologically relevant hydrostatic pressure on CHO cell batch culture
title_fullStr	Investigating the influence of physiologically relevant hydrostatic pressure on CHO cell batch culture
title_full_unstemmed	Investigating the influence of physiologically relevant hydrostatic pressure on CHO cell batch culture
title_sort	investigating the influence of physiologically relevant hydrostatic pressure on cho cell batch culture
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/da12497fc0964278b9a19b664192776e
work_keys_str_mv	AT menglinshang investigatingtheinfluenceofphysiologicallyrelevanthydrostaticpressureonchocellbatchculture AT taehongkwon investigatingtheinfluenceofphysiologicallyrelevanthydrostaticpressureonchocellbatchculture AT jeanfrancoisphamel investigatingtheinfluenceofphysiologicallyrelevanthydrostaticpressureonchocellbatchculture AT chweetecklim investigatingtheinfluenceofphysiologicallyrelevanthydrostaticpressureonchocellbatchculture AT beeluankhoo investigatingtheinfluenceofphysiologicallyrelevanthydrostaticpressureonchocellbatchculture AT jongyoonhan investigatingtheinfluenceofphysiologicallyrelevanthydrostaticpressureonchocellbatchculture
_version_	1718395254408740864

Investigating the influence of physiologically relevant hydrostatic pressure on CHO cell batch culture

Ejemplares similares