Evaluation of Methylation at Promoter Regions of Long Non-coding RNAs in Patients with Acute Lymphoblastic Leukemia

Background: Acute lymphoblastic leukemia (ALL) is a fetal hematologic disorder that is mostly observed in children. Both B and T lymphocytes have been reported to play a role in ALL etiology. Long non-coding RNAs (lncRNAs) are large regulatory molecules with more than 200nucleotides that participate...

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Autores principales:	Zohreh Mousavi, Saeid Ghorbian, Azim Rezamand, Leyla Roshangar, Behboud Jafari
Formato:	article
Lenguaje:	EN
Publicado:	Tabriz University of Medical Sciences 2021
Materias:	long non-coding rna acute lymphoblastic leukemia oncogenesis methylation Pharmacy and materia medica RS1-441
Acceso en línea:	https://doaj.org/article/da143d311d3b4653ab164991d67c4862
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spelling	oai:doaj.org-article:da143d311d3b4653ab164991d67c48622021-11-29T05:53:31ZEvaluation of Methylation at Promoter Regions of Long Non-coding RNAs in Patients with Acute Lymphoblastic Leukemia2383-288610.34172/PS.2021.18https://doaj.org/article/da143d311d3b4653ab164991d67c48622021-12-01T00:00:00Zhttps://ps.tbzmed.ac.ir/PDF/ps-27-552.pdfhttps://doaj.org/toc/2383-2886Background: Acute lymphoblastic leukemia (ALL) is a fetal hematologic disorder that is mostly observed in children. Both B and T lymphocytes have been reported to play a role in ALL etiology. Long non-coding RNAs (lncRNAs) are large regulatory molecules with more than 200nucleotides that participate in various cellular processes. Methylation at the promoter regions of these regulatory molecules has been reported to vary between ALL patients and healthy controls. This study aimed to evaluate methylation status at promoter regions of lncRNAs between these two groups. Methods: In the current study, 80 ALL patients and 80 healthy controls were enrolled. The intravenous blood samples were obtained from all patients and controls. The extracted DNA from blood samples underwent sodium bisulfite treatment. Thereafter, methylation levels in the promoter regions of lncRNAs RP11-137H2.4, RP11-624c23.1, RP11-203E8, RP11-446E9, andRP11-68118.10 were evaluated using methylation specific‑high resolution melting (MS‑HRM). Moreover, the receiver operating characteristic curve (ROC) analysis was performed to examine the sensitivity and specificity of the tests. Results: The methylation levels of all studied lncRNAs including RP11-137H2.4, RP11-624c23.1, RP11-203E8, RP11-446E9, and RP11-68118.10 were significantly increased (p<0.05). ROC curve analysis also showed that all lncRNAs could be used as diagnostic markers. Conclusion: This study showed that methylation alterations of lncRNAs could be considered as novel biomarkers for early detection of ALL. Furthermore, owing to the possible role of studied lncRNAs as tumor suppressors, they could be reliable treatment targets for methylation modifications. Further research is still required to elucidate the role of these lncRNAs in ALL etiology.Zohreh MousaviSaeid GhorbianAzim RezamandLeyla RoshangarBehboud JafariTabriz University of Medical Sciencesarticlelong non-coding rnaacute lymphoblastic leukemiaoncogenesismethylationPharmacy and materia medicaRS1-441ENPharmaceutical Sciences, Vol 27, Iss 4, Pp 552-559 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	long non-coding rna acute lymphoblastic leukemia oncogenesis methylation Pharmacy and materia medica RS1-441
spellingShingle	long non-coding rna acute lymphoblastic leukemia oncogenesis methylation Pharmacy and materia medica RS1-441 Zohreh Mousavi Saeid Ghorbian Azim Rezamand Leyla Roshangar Behboud Jafari Evaluation of Methylation at Promoter Regions of Long Non-coding RNAs in Patients with Acute Lymphoblastic Leukemia
description	Background: Acute lymphoblastic leukemia (ALL) is a fetal hematologic disorder that is mostly observed in children. Both B and T lymphocytes have been reported to play a role in ALL etiology. Long non-coding RNAs (lncRNAs) are large regulatory molecules with more than 200nucleotides that participate in various cellular processes. Methylation at the promoter regions of these regulatory molecules has been reported to vary between ALL patients and healthy controls. This study aimed to evaluate methylation status at promoter regions of lncRNAs between these two groups. Methods: In the current study, 80 ALL patients and 80 healthy controls were enrolled. The intravenous blood samples were obtained from all patients and controls. The extracted DNA from blood samples underwent sodium bisulfite treatment. Thereafter, methylation levels in the promoter regions of lncRNAs RP11-137H2.4, RP11-624c23.1, RP11-203E8, RP11-446E9, andRP11-68118.10 were evaluated using methylation specific‑high resolution melting (MS‑HRM). Moreover, the receiver operating characteristic curve (ROC) analysis was performed to examine the sensitivity and specificity of the tests. Results: The methylation levels of all studied lncRNAs including RP11-137H2.4, RP11-624c23.1, RP11-203E8, RP11-446E9, and RP11-68118.10 were significantly increased (p<0.05). ROC curve analysis also showed that all lncRNAs could be used as diagnostic markers. Conclusion: This study showed that methylation alterations of lncRNAs could be considered as novel biomarkers for early detection of ALL. Furthermore, owing to the possible role of studied lncRNAs as tumor suppressors, they could be reliable treatment targets for methylation modifications. Further research is still required to elucidate the role of these lncRNAs in ALL etiology.
format	article
author	Zohreh Mousavi Saeid Ghorbian Azim Rezamand Leyla Roshangar Behboud Jafari
author_facet	Zohreh Mousavi Saeid Ghorbian Azim Rezamand Leyla Roshangar Behboud Jafari
author_sort	Zohreh Mousavi
title	Evaluation of Methylation at Promoter Regions of Long Non-coding RNAs in Patients with Acute Lymphoblastic Leukemia
title_short	Evaluation of Methylation at Promoter Regions of Long Non-coding RNAs in Patients with Acute Lymphoblastic Leukemia
title_full	Evaluation of Methylation at Promoter Regions of Long Non-coding RNAs in Patients with Acute Lymphoblastic Leukemia
title_fullStr	Evaluation of Methylation at Promoter Regions of Long Non-coding RNAs in Patients with Acute Lymphoblastic Leukemia
title_full_unstemmed	Evaluation of Methylation at Promoter Regions of Long Non-coding RNAs in Patients with Acute Lymphoblastic Leukemia
title_sort	evaluation of methylation at promoter regions of long non-coding rnas in patients with acute lymphoblastic leukemia
publisher	Tabriz University of Medical Sciences
publishDate	2021
url	https://doaj.org/article/da143d311d3b4653ab164991d67c4862
work_keys_str_mv	AT zohrehmousavi evaluationofmethylationatpromoterregionsoflongnoncodingrnasinpatientswithacutelymphoblasticleukemia AT saeidghorbian evaluationofmethylationatpromoterregionsoflongnoncodingrnasinpatientswithacutelymphoblasticleukemia AT azimrezamand evaluationofmethylationatpromoterregionsoflongnoncodingrnasinpatientswithacutelymphoblasticleukemia AT leylaroshangar evaluationofmethylationatpromoterregionsoflongnoncodingrnasinpatientswithacutelymphoblasticleukemia AT behboudjafari evaluationofmethylationatpromoterregionsoflongnoncodingrnasinpatientswithacutelymphoblasticleukemia
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Evaluation of Methylation at Promoter Regions of Long Non-coding RNAs in Patients with Acute Lymphoblastic Leukemia

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