Puerarin enhances intestinal function in piglets infected with porcine epidemic diarrhea virus

Abstract Puerarin has been reported to be an excellent antioxidant, anti-inflammatory and antimicrobial agent, but the potential effect of puerarin on porcine epidemic diarrhea virus (PEDV) is unclear. This study aimed to determine whether puerarin could alleviate intestinal injury in piglets infect...

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Autores principales:	Mengjun Wu, Dan Yi, Qian Zhang, Tao Wu, Kui Yu, Meng Peng, Lei Wang, Di Zhao, Yongqing Hou, Guoyao Wu
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/da162a262476408aa4211c3012220231
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spelling	oai:doaj.org-article:da162a262476408aa4211c30122202312021-12-02T17:04:07ZPuerarin enhances intestinal function in piglets infected with porcine epidemic diarrhea virus10.1038/s41598-021-85880-52045-2322https://doaj.org/article/da162a262476408aa4211c30122202312021-03-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-85880-5https://doaj.org/toc/2045-2322Abstract Puerarin has been reported to be an excellent antioxidant, anti-inflammatory and antimicrobial agent, but the potential effect of puerarin on porcine epidemic diarrhea virus (PEDV) is unclear. This study aimed to determine whether puerarin could alleviate intestinal injury in piglets infected with PEDV. A PEDV (Yunnan province strain) infection model was applied to 7-day-old piglets at 104.5 TCID50 (50% tissue culture infectious dose). Piglets were orally administered with puerarin at the dosage of 0.5 mg/kg body weight from day 5 to day 9. On day 9 of the trial, piglets were inoculated orally with PEDV. Three days later, jugular vein blood and intestinal samples were collected. Results showed puerarin reduced morbidity of piglets infected with PEDV. In addition, puerarin reduced the activities of aspartate aminotransferase and alkaline phosphatase, the ratio of serum aspartate aminotransferase to serum alanine aminotransferase, the number of white blood cells and neutrophils, and the plasma concentrations of interleukin-6, interleukin-8 and tumor necrosis factor-α, as well as protein abundances of heat shock protein-70 in PEDV-infected piglets. Moreover, puerarin increased D-xylose concentration but decreased intestinal fatty acid-binding protein concentration and diamine oxidase activity in the plasma of piglets infected with PEDV. Puerarin increased the activities of total superoxide dismutase, glutathione peroxidase and catalase, while decreasing the activities of myeloperoxidase and concentration of hydrogen peroxide in both the intestine and plasma of PEDV-infected piglets. Puerarin decreased mRNA levels of glutathione S-transferase omega 2 but increased the levels of nuclear factor erythroid 2-related factor 2. Furthermore, puerarin increased the abundance of total eubacteria (16S rRNA), Enterococcus genus, Lactobacillus genus and Enterobacteriaceae family in the intestine, but reduced the abundance of Clostridium coccoides in the caecum. These data indicate puerarin improved intestinal function in piglets infected by PEDV and may be a promising supplement for the prevention of PEDV infection.Mengjun WuDan YiQian ZhangTao WuKui YuMeng PengLei WangDi ZhaoYongqing HouGuoyao WuNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-14 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Mengjun Wu Dan Yi Qian Zhang Tao Wu Kui Yu Meng Peng Lei Wang Di Zhao Yongqing Hou Guoyao Wu Puerarin enhances intestinal function in piglets infected with porcine epidemic diarrhea virus
description	Abstract Puerarin has been reported to be an excellent antioxidant, anti-inflammatory and antimicrobial agent, but the potential effect of puerarin on porcine epidemic diarrhea virus (PEDV) is unclear. This study aimed to determine whether puerarin could alleviate intestinal injury in piglets infected with PEDV. A PEDV (Yunnan province strain) infection model was applied to 7-day-old piglets at 104.5 TCID50 (50% tissue culture infectious dose). Piglets were orally administered with puerarin at the dosage of 0.5 mg/kg body weight from day 5 to day 9. On day 9 of the trial, piglets were inoculated orally with PEDV. Three days later, jugular vein blood and intestinal samples were collected. Results showed puerarin reduced morbidity of piglets infected with PEDV. In addition, puerarin reduced the activities of aspartate aminotransferase and alkaline phosphatase, the ratio of serum aspartate aminotransferase to serum alanine aminotransferase, the number of white blood cells and neutrophils, and the plasma concentrations of interleukin-6, interleukin-8 and tumor necrosis factor-α, as well as protein abundances of heat shock protein-70 in PEDV-infected piglets. Moreover, puerarin increased D-xylose concentration but decreased intestinal fatty acid-binding protein concentration and diamine oxidase activity in the plasma of piglets infected with PEDV. Puerarin increased the activities of total superoxide dismutase, glutathione peroxidase and catalase, while decreasing the activities of myeloperoxidase and concentration of hydrogen peroxide in both the intestine and plasma of PEDV-infected piglets. Puerarin decreased mRNA levels of glutathione S-transferase omega 2 but increased the levels of nuclear factor erythroid 2-related factor 2. Furthermore, puerarin increased the abundance of total eubacteria (16S rRNA), Enterococcus genus, Lactobacillus genus and Enterobacteriaceae family in the intestine, but reduced the abundance of Clostridium coccoides in the caecum. These data indicate puerarin improved intestinal function in piglets infected by PEDV and may be a promising supplement for the prevention of PEDV infection.
format	article
author	Mengjun Wu Dan Yi Qian Zhang Tao Wu Kui Yu Meng Peng Lei Wang Di Zhao Yongqing Hou Guoyao Wu
author_facet	Mengjun Wu Dan Yi Qian Zhang Tao Wu Kui Yu Meng Peng Lei Wang Di Zhao Yongqing Hou Guoyao Wu
author_sort	Mengjun Wu
title	Puerarin enhances intestinal function in piglets infected with porcine epidemic diarrhea virus
title_short	Puerarin enhances intestinal function in piglets infected with porcine epidemic diarrhea virus
title_full	Puerarin enhances intestinal function in piglets infected with porcine epidemic diarrhea virus
title_fullStr	Puerarin enhances intestinal function in piglets infected with porcine epidemic diarrhea virus
title_full_unstemmed	Puerarin enhances intestinal function in piglets infected with porcine epidemic diarrhea virus
title_sort	puerarin enhances intestinal function in piglets infected with porcine epidemic diarrhea virus
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/da162a262476408aa4211c3012220231
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Puerarin enhances intestinal function in piglets infected with porcine epidemic diarrhea virus

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