Megakaryocytic differentiation of K562 cells induced by PMA reduced the activity of respiratory chain complex IV.

Mitochondria are involved in the regulation of cell differentiation processes, but its function changes and molecular mechanisms are not yet clear. In this study, we found that mitochondrial functions changed obviously when K562 cells were induced to megakaryocytic differentiation by phorbol 12-myri...

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Autores principales:	Rui Huang, Long Zhao, Hui Chen, Rong-Hua Yin, Chang-Yan Li, Yi-Qun Zhan, Jian-Hong Zhang, Chang-hui Ge, Miao Yu, Xiao-Ming Yang
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2014
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/da196f3df5944096a9a79c7ad718b694
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spelling	oai:doaj.org-article:da196f3df5944096a9a79c7ad718b6942021-11-18T08:19:53ZMegakaryocytic differentiation of K562 cells induced by PMA reduced the activity of respiratory chain complex IV.1932-620310.1371/journal.pone.0096246https://doaj.org/article/da196f3df5944096a9a79c7ad718b6942014-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24817082/?tool=EBIhttps://doaj.org/toc/1932-6203Mitochondria are involved in the regulation of cell differentiation processes, but its function changes and molecular mechanisms are not yet clear. In this study, we found that mitochondrial functions changed obviously when K562 cells were induced to megakaryocytic differentiation by phorbol 12-myristate 13-acetate (PMA). During the cell differentiation, the reactive oxygen species (ROS) level was increased, mitochondrial membrane potential declined and respiratory chain complex IV activity was decreased. Treatment with specific inhibitor of mitochondrial respiratory chain complex IV led to a significant inhibition in mitochondrial membrane potential and reduction of PMA-induced cell differentiation. However, treatment with cyclosporine A, a stabilization reagent of mitochondrial membrane potential, did not improve the down-regulation of mitochondrial respiratory chain complex IV induced by PMA. Furthermore, we found that the level of the complex IV core subunit COX3 and mitochondrial transport-related proteins Tim9 and Tim10 were decreased during the differentiation of K562 cells induced by PMA, suggesting an important role of these factors in mitochondrial functional changes. Our results suggest that changes in mitochondrial functions are involved in the PMA-induced K562 cell differentiation process, and the maintenance of the steady-state of mitochondrial functions plays a critical role in the regulation of cell differentiation.Rui HuangLong ZhaoHui ChenRong-Hua YinChang-Yan LiYi-Qun ZhanJian-Hong ZhangChang-hui GeMiao YuXiao-Ming YangPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 9, Iss 5, p e96246 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Rui Huang Long Zhao Hui Chen Rong-Hua Yin Chang-Yan Li Yi-Qun Zhan Jian-Hong Zhang Chang-hui Ge Miao Yu Xiao-Ming Yang Megakaryocytic differentiation of K562 cells induced by PMA reduced the activity of respiratory chain complex IV.
description	Mitochondria are involved in the regulation of cell differentiation processes, but its function changes and molecular mechanisms are not yet clear. In this study, we found that mitochondrial functions changed obviously when K562 cells were induced to megakaryocytic differentiation by phorbol 12-myristate 13-acetate (PMA). During the cell differentiation, the reactive oxygen species (ROS) level was increased, mitochondrial membrane potential declined and respiratory chain complex IV activity was decreased. Treatment with specific inhibitor of mitochondrial respiratory chain complex IV led to a significant inhibition in mitochondrial membrane potential and reduction of PMA-induced cell differentiation. However, treatment with cyclosporine A, a stabilization reagent of mitochondrial membrane potential, did not improve the down-regulation of mitochondrial respiratory chain complex IV induced by PMA. Furthermore, we found that the level of the complex IV core subunit COX3 and mitochondrial transport-related proteins Tim9 and Tim10 were decreased during the differentiation of K562 cells induced by PMA, suggesting an important role of these factors in mitochondrial functional changes. Our results suggest that changes in mitochondrial functions are involved in the PMA-induced K562 cell differentiation process, and the maintenance of the steady-state of mitochondrial functions plays a critical role in the regulation of cell differentiation.
format	article
author	Rui Huang Long Zhao Hui Chen Rong-Hua Yin Chang-Yan Li Yi-Qun Zhan Jian-Hong Zhang Chang-hui Ge Miao Yu Xiao-Ming Yang
author_facet	Rui Huang Long Zhao Hui Chen Rong-Hua Yin Chang-Yan Li Yi-Qun Zhan Jian-Hong Zhang Chang-hui Ge Miao Yu Xiao-Ming Yang
author_sort	Rui Huang
title	Megakaryocytic differentiation of K562 cells induced by PMA reduced the activity of respiratory chain complex IV.
title_short	Megakaryocytic differentiation of K562 cells induced by PMA reduced the activity of respiratory chain complex IV.
title_full	Megakaryocytic differentiation of K562 cells induced by PMA reduced the activity of respiratory chain complex IV.
title_fullStr	Megakaryocytic differentiation of K562 cells induced by PMA reduced the activity of respiratory chain complex IV.
title_full_unstemmed	Megakaryocytic differentiation of K562 cells induced by PMA reduced the activity of respiratory chain complex IV.
title_sort	megakaryocytic differentiation of k562 cells induced by pma reduced the activity of respiratory chain complex iv.
publisher	Public Library of Science (PLoS)
publishDate	2014
url	https://doaj.org/article/da196f3df5944096a9a79c7ad718b694
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Megakaryocytic differentiation of K562 cells induced by PMA reduced the activity of respiratory chain complex IV.

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