Role of fibronectin in the adhesion of Acinetobacter baumannii to host cells.

Adhesion to host cells is an initial and important step in Acinetobacter baumannii pathogenesis. However, there is relatively little information on the mechanisms by which A. baumannii binds to and interacts with host cells. Adherence to extracellular matrix proteins, such as fibronectin, affords pa...

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Autores principales:	Younes Smani, Michael J McConnell, Jerónimo Pachón
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2012
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/da1dada801cb4a0da4ee9c61a74c538c
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spelling	oai:doaj.org-article:da1dada801cb4a0da4ee9c61a74c538c2021-11-18T07:22:22ZRole of fibronectin in the adhesion of Acinetobacter baumannii to host cells.1932-620310.1371/journal.pone.0033073https://doaj.org/article/da1dada801cb4a0da4ee9c61a74c538c2012-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/22514602/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203Adhesion to host cells is an initial and important step in Acinetobacter baumannii pathogenesis. However, there is relatively little information on the mechanisms by which A. baumannii binds to and interacts with host cells. Adherence to extracellular matrix proteins, such as fibronectin, affords pathogens with a mechanism to invade epithelial cells. Here, we found that A. baumannii adheres more avidly to immobilized fibronectin than to control protein. Free fibronectin used as a competitor resulted in dose-dependent decreased binding of A. baumannii to fibronectin. Three outer membrane preparations (OMPs) were identified as fibronectin binding proteins (FBPs): OMPA, TonB-dependent copper receptor, and 34 kDa OMP. Moreover, we demonstrated that fibronectin inhibition and neutralization by specific antibody prevented significantly the adhesion of A. baumannii to human lung epithelial cells (A549 cells). Similarly, A. baumannii OMPA neutralization by specific antibody decreased significantly the adhesion of A. baumannii to A549 cells. These data indicate that FBPs are key adhesins that mediate binding of A. baumannii to human lung epithelial cells through interaction with fibronectin on the surface of these host cells.Younes SmaniMichael J McConnellJerónimo PachónPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 7, Iss 4, p e33073 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Younes Smani Michael J McConnell Jerónimo Pachón Role of fibronectin in the adhesion of Acinetobacter baumannii to host cells.
description	Adhesion to host cells is an initial and important step in Acinetobacter baumannii pathogenesis. However, there is relatively little information on the mechanisms by which A. baumannii binds to and interacts with host cells. Adherence to extracellular matrix proteins, such as fibronectin, affords pathogens with a mechanism to invade epithelial cells. Here, we found that A. baumannii adheres more avidly to immobilized fibronectin than to control protein. Free fibronectin used as a competitor resulted in dose-dependent decreased binding of A. baumannii to fibronectin. Three outer membrane preparations (OMPs) were identified as fibronectin binding proteins (FBPs): OMPA, TonB-dependent copper receptor, and 34 kDa OMP. Moreover, we demonstrated that fibronectin inhibition and neutralization by specific antibody prevented significantly the adhesion of A. baumannii to human lung epithelial cells (A549 cells). Similarly, A. baumannii OMPA neutralization by specific antibody decreased significantly the adhesion of A. baumannii to A549 cells. These data indicate that FBPs are key adhesins that mediate binding of A. baumannii to human lung epithelial cells through interaction with fibronectin on the surface of these host cells.
format	article
author	Younes Smani Michael J McConnell Jerónimo Pachón
author_facet	Younes Smani Michael J McConnell Jerónimo Pachón
author_sort	Younes Smani
title	Role of fibronectin in the adhesion of Acinetobacter baumannii to host cells.
title_short	Role of fibronectin in the adhesion of Acinetobacter baumannii to host cells.
title_full	Role of fibronectin in the adhesion of Acinetobacter baumannii to host cells.
title_fullStr	Role of fibronectin in the adhesion of Acinetobacter baumannii to host cells.
title_full_unstemmed	Role of fibronectin in the adhesion of Acinetobacter baumannii to host cells.
title_sort	role of fibronectin in the adhesion of acinetobacter baumannii to host cells.
publisher	Public Library of Science (PLoS)
publishDate	2012
url	https://doaj.org/article/da1dada801cb4a0da4ee9c61a74c538c
work_keys_str_mv	AT younessmani roleoffibronectinintheadhesionofacinetobacterbaumanniitohostcells AT michaeljmcconnell roleoffibronectinintheadhesionofacinetobacterbaumanniitohostcells AT jeronimopachon roleoffibronectinintheadhesionofacinetobacterbaumanniitohostcells
_version_	1718423566216593408

Role of fibronectin in the adhesion of Acinetobacter baumannii to host cells.

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