Modified ELISA for Ultrasensitive Diagnosis

An enzyme-linked immunosorbent assay (ELISA) can be used for quantitative measurement of proteins, and improving the detection sensitivity to the ultrasensitive level would facilitate the diagnosis of various diseases. In the present review article, we first define the term ‘ultrasensitive’. We foll...

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Autores principales:	Naoko Tsurusawa, Jyunhao Chang, Mayuri Namba, Daiki Makioka, Sou Yamura, Kanako Iha, Yuta Kyosei, Satoshi Watabe, Teruki Yoshimura, Etsuro Ito
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	diagnosis infectious disease lifestyle-related disease ultrasensitive ELISA thio-NAD cycling Medicine R
Acceso en línea:	https://doaj.org/article/da262e53f82f48c3ad49a709bdfcbaa0
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id	oai:doaj.org-article:da262e53f82f48c3ad49a709bdfcbaa0
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spelling	oai:doaj.org-article:da262e53f82f48c3ad49a709bdfcbaa02021-11-11T17:48:09ZModified ELISA for Ultrasensitive Diagnosis10.3390/jcm102151972077-0383https://doaj.org/article/da262e53f82f48c3ad49a709bdfcbaa02021-11-01T00:00:00Zhttps://www.mdpi.com/2077-0383/10/21/5197https://doaj.org/toc/2077-0383An enzyme-linked immunosorbent assay (ELISA) can be used for quantitative measurement of proteins, and improving the detection sensitivity to the ultrasensitive level would facilitate the diagnosis of various diseases. In the present review article, we first define the term ‘ultrasensitive’. We follow this with a survey and discussion of the current literature regarding modified ELISA methods with ultrasensitive detection and their application for diagnosis. Finally, we introduce our own newly devised system for ultrasensitive ELISA combined with thionicotinamide adenine dinucleotide cycling and its application for the diagnosis of infectious diseases and lifestyle-related diseases. The aim of the present article is to expand the application of ultrasensitive ELISAs in the medical and biological fields.Naoko TsurusawaJyunhao ChangMayuri NambaDaiki MakiokaSou YamuraKanako IhaYuta KyoseiSatoshi WatabeTeruki YoshimuraEtsuro ItoMDPI AGarticlediagnosisinfectious diseaselifestyle-related diseaseultrasensitive ELISAthio-NAD cyclingMedicineRENJournal of Clinical Medicine, Vol 10, Iss 5197, p 5197 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	diagnosis infectious disease lifestyle-related disease ultrasensitive ELISA thio-NAD cycling Medicine R
spellingShingle	diagnosis infectious disease lifestyle-related disease ultrasensitive ELISA thio-NAD cycling Medicine R Naoko Tsurusawa Jyunhao Chang Mayuri Namba Daiki Makioka Sou Yamura Kanako Iha Yuta Kyosei Satoshi Watabe Teruki Yoshimura Etsuro Ito Modified ELISA for Ultrasensitive Diagnosis
description	An enzyme-linked immunosorbent assay (ELISA) can be used for quantitative measurement of proteins, and improving the detection sensitivity to the ultrasensitive level would facilitate the diagnosis of various diseases. In the present review article, we first define the term ‘ultrasensitive’. We follow this with a survey and discussion of the current literature regarding modified ELISA methods with ultrasensitive detection and their application for diagnosis. Finally, we introduce our own newly devised system for ultrasensitive ELISA combined with thionicotinamide adenine dinucleotide cycling and its application for the diagnosis of infectious diseases and lifestyle-related diseases. The aim of the present article is to expand the application of ultrasensitive ELISAs in the medical and biological fields.
format	article
author	Naoko Tsurusawa Jyunhao Chang Mayuri Namba Daiki Makioka Sou Yamura Kanako Iha Yuta Kyosei Satoshi Watabe Teruki Yoshimura Etsuro Ito
author_facet	Naoko Tsurusawa Jyunhao Chang Mayuri Namba Daiki Makioka Sou Yamura Kanako Iha Yuta Kyosei Satoshi Watabe Teruki Yoshimura Etsuro Ito
author_sort	Naoko Tsurusawa
title	Modified ELISA for Ultrasensitive Diagnosis
title_short	Modified ELISA for Ultrasensitive Diagnosis
title_full	Modified ELISA for Ultrasensitive Diagnosis
title_fullStr	Modified ELISA for Ultrasensitive Diagnosis
title_full_unstemmed	Modified ELISA for Ultrasensitive Diagnosis
title_sort	modified elisa for ultrasensitive diagnosis
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/da262e53f82f48c3ad49a709bdfcbaa0
work_keys_str_mv	AT naokotsurusawa modifiedelisaforultrasensitivediagnosis AT jyunhaochang modifiedelisaforultrasensitivediagnosis AT mayurinamba modifiedelisaforultrasensitivediagnosis AT daikimakioka modifiedelisaforultrasensitivediagnosis AT souyamura modifiedelisaforultrasensitivediagnosis AT kanakoiha modifiedelisaforultrasensitivediagnosis AT yutakyosei modifiedelisaforultrasensitivediagnosis AT satoshiwatabe modifiedelisaforultrasensitivediagnosis AT terukiyoshimura modifiedelisaforultrasensitivediagnosis AT etsuroito modifiedelisaforultrasensitivediagnosis
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Modified ELISA for Ultrasensitive Diagnosis

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