The hidden habit of the entomopathogenic fungus Beauveria bassiana: first demonstration of vertical plant transmission.

Beauveria bassiana strain 04/01-Tip, obtained from a larva of the opium poppy stem gall wasp Iraella luteipes (Hymenoptera; Cynipidae), endophytically colonizes opium poppy (Papaver somniferum L.) plants and protects them against this pest. The goal of this study was to monitor the dynamics of endop...

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Autores principales:	Enrique Quesada-Moraga, Cristina López-Díaz, Blanca Beatriz Landa
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2014
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/da3600cde2244d28a684b6d3fd95383e
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spelling	oai:doaj.org-article:da3600cde2244d28a684b6d3fd95383e2021-11-18T08:32:22ZThe hidden habit of the entomopathogenic fungus Beauveria bassiana: first demonstration of vertical plant transmission.1932-620310.1371/journal.pone.0089278https://doaj.org/article/da3600cde2244d28a684b6d3fd95383e2014-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24551242/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203Beauveria bassiana strain 04/01-Tip, obtained from a larva of the opium poppy stem gall wasp Iraella luteipes (Hymenoptera; Cynipidae), endophytically colonizes opium poppy (Papaver somniferum L.) plants and protects them against this pest. The goal of this study was to monitor the dynamics of endophytic colonization of opium poppy by B. bassiana after the fungus was applied to the seed and to ascertain whether the fungus is transmitted vertically via seeds. Using a species-specific nested PCR protocol and DNA extracted from surface-sterilised leaf pieces or seeds of B. bassiana-inoculated opium poppy plants, the fungus was detected within the plant beginning at the growth stage of rosette building and them throughout the entire plant growth cycle (about 120-140 days after sowing). The fungus was also detected in seeds from 50% of the capsules sampled. Seeds that showed positive amplification for B. bassiana were planted in sterile soil and the endophyte was again detected in more than 42% of the plants sampled during all plant growth stages. Beauveria bassiana was transmitted to seeds in 25% of the plants from the second generation that formed a mature capsule. These results demonstrate for the first time the vertical transmission of an entomopathogenic fungus from endophytically colonised maternal plants. This information is crucial to better understand the ecological role of entomopathogenic fungi as plant endophytes and may allow development of a sustainable and cost effective strategy for I. luteipes management in P. somniferum.Enrique Quesada-MoragaCristina López-DíazBlanca Beatriz LandaPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 9, Iss 2, p e89278 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Enrique Quesada-Moraga Cristina López-Díaz Blanca Beatriz Landa The hidden habit of the entomopathogenic fungus Beauveria bassiana: first demonstration of vertical plant transmission.
description	Beauveria bassiana strain 04/01-Tip, obtained from a larva of the opium poppy stem gall wasp Iraella luteipes (Hymenoptera; Cynipidae), endophytically colonizes opium poppy (Papaver somniferum L.) plants and protects them against this pest. The goal of this study was to monitor the dynamics of endophytic colonization of opium poppy by B. bassiana after the fungus was applied to the seed and to ascertain whether the fungus is transmitted vertically via seeds. Using a species-specific nested PCR protocol and DNA extracted from surface-sterilised leaf pieces or seeds of B. bassiana-inoculated opium poppy plants, the fungus was detected within the plant beginning at the growth stage of rosette building and them throughout the entire plant growth cycle (about 120-140 days after sowing). The fungus was also detected in seeds from 50% of the capsules sampled. Seeds that showed positive amplification for B. bassiana were planted in sterile soil and the endophyte was again detected in more than 42% of the plants sampled during all plant growth stages. Beauveria bassiana was transmitted to seeds in 25% of the plants from the second generation that formed a mature capsule. These results demonstrate for the first time the vertical transmission of an entomopathogenic fungus from endophytically colonised maternal plants. This information is crucial to better understand the ecological role of entomopathogenic fungi as plant endophytes and may allow development of a sustainable and cost effective strategy for I. luteipes management in P. somniferum.
format	article
author	Enrique Quesada-Moraga Cristina López-Díaz Blanca Beatriz Landa
author_facet	Enrique Quesada-Moraga Cristina López-Díaz Blanca Beatriz Landa
author_sort	Enrique Quesada-Moraga
title	The hidden habit of the entomopathogenic fungus Beauveria bassiana: first demonstration of vertical plant transmission.
title_short	The hidden habit of the entomopathogenic fungus Beauveria bassiana: first demonstration of vertical plant transmission.
title_full	The hidden habit of the entomopathogenic fungus Beauveria bassiana: first demonstration of vertical plant transmission.
title_fullStr	The hidden habit of the entomopathogenic fungus Beauveria bassiana: first demonstration of vertical plant transmission.
title_full_unstemmed	The hidden habit of the entomopathogenic fungus Beauveria bassiana: first demonstration of vertical plant transmission.
title_sort	hidden habit of the entomopathogenic fungus beauveria bassiana: first demonstration of vertical plant transmission.
publisher	Public Library of Science (PLoS)
publishDate	2014
url	https://doaj.org/article/da3600cde2244d28a684b6d3fd95383e
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The hidden habit of the entomopathogenic fungus Beauveria bassiana: first demonstration of vertical plant transmission.

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