In vitro evaluation of anticancer nanomedicines based on doxorubicin and amphiphilic Y-shaped copolymers

Di Li,1,2,* Jian Xun Ding,1,3,* Zhao Hui Tang,1 Hai Sun,1 Xiu Li Zhuang,1 Jing Zhe Xu,2 Xue Si Chen1 1Key Laboratory of Polymer Ecomaterials, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, 2Department of Chemistry, Yanbian University, Yanji, 3Graduate University of...

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Autores principales:	Li D, Ding JX, Tang ZH, Sun H, Zhuang XL, Xu JZ, Chen XS
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2012
Materias:	Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/da38869c5db3439f8d7fc512aa507650
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spelling	oai:doaj.org-article:da38869c5db3439f8d7fc512aa5076502021-12-02T05:26:35ZIn vitro evaluation of anticancer nanomedicines based on doxorubicin and amphiphilic Y-shaped copolymers1176-91141178-2013https://doaj.org/article/da38869c5db3439f8d7fc512aa5076502012-05-01T00:00:00Zhttp://www.dovepress.com/in-vitro-evaluation-of-anticancer-nanomedicines-based-on-doxorubicin-a-a10003https://doaj.org/toc/1176-9114https://doaj.org/toc/1178-2013Di Li,1,2,* Jian Xun Ding,1,3,* Zhao Hui Tang,1 Hai Sun,1 Xiu Li Zhuang,1 Jing Zhe Xu,2 Xue Si Chen1 1Key Laboratory of Polymer Ecomaterials, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, 2Department of Chemistry, Yanbian University, Yanji, 3Graduate University of Chinese Academy of Sciences, Beijing, China *These authors contributed equally to this workAbstract: Four monomethoxy poly(ethylene glycol)-poly(L-lactide-co-glycolide)2 (mPEG-P(LA-co-GA)2) copolymers were synthesized by ring-opening polymerization of L-lactide and glycolide with double hydroxyl functionalized mPEG (mPEG-(OH)2) as macroinitiator and stannous octoate as catalyst. The copolymers self-assembled into nanoscale micellar/vesicular aggregations in phosphate buffer at pH 7.4. Doxorubicin (DOX), an anthracycline anticancer drug, was loaded into the micellar/vesicular nanoparticles, yielding micellar/vesicular nanomedicines. The in vitro release behaviors could be adjusted by content of hydrophobic polyester and pH of the release medium. In vitro cell experiments showed that the intracellular DOX release could be adjusted by content of P(LA-co-GA), and the nanomedicines displayed effective proliferation inhibition against Henrietta Lacks&rsquo;s cells with different culture times. Hemolysis tests indicated that the copolymers were hemocompatible, and the presence of copolymers could reduce the hemolysis ratio of DOX significantly. These results suggested that the novel anticancer nanomedicines based on DOX and amphiphilic Y-shaped copolymers were attractive candidates as tumor tissular and intracellular targeting drug delivery systems in vivo, with enhanced stability during circulation and accelerated drug release at the target sites.Keywords: amphiphilic Y-shaped copolymer, anticancer nanomedicine, cellular proliferation inhibition, doxorubicinLi DDing JXTang ZHSun HZhuang XLXu JZChen XSDove Medical PressarticleMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2012, Iss default, Pp 2687-2697 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine (General) R5-920
spellingShingle	Medicine (General) R5-920 Li D Ding JX Tang ZH Sun H Zhuang XL Xu JZ Chen XS In vitro evaluation of anticancer nanomedicines based on doxorubicin and amphiphilic Y-shaped copolymers
description	Di Li,1,2,* Jian Xun Ding,1,3,* Zhao Hui Tang,1 Hai Sun,1 Xiu Li Zhuang,1 Jing Zhe Xu,2 Xue Si Chen1 1Key Laboratory of Polymer Ecomaterials, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, 2Department of Chemistry, Yanbian University, Yanji, 3Graduate University of Chinese Academy of Sciences, Beijing, China *These authors contributed equally to this workAbstract: Four monomethoxy poly(ethylene glycol)-poly(L-lactide-co-glycolide)2 (mPEG-P(LA-co-GA)2) copolymers were synthesized by ring-opening polymerization of L-lactide and glycolide with double hydroxyl functionalized mPEG (mPEG-(OH)2) as macroinitiator and stannous octoate as catalyst. The copolymers self-assembled into nanoscale micellar/vesicular aggregations in phosphate buffer at pH 7.4. Doxorubicin (DOX), an anthracycline anticancer drug, was loaded into the micellar/vesicular nanoparticles, yielding micellar/vesicular nanomedicines. The in vitro release behaviors could be adjusted by content of hydrophobic polyester and pH of the release medium. In vitro cell experiments showed that the intracellular DOX release could be adjusted by content of P(LA-co-GA), and the nanomedicines displayed effective proliferation inhibition against Henrietta Lacks&rsquo;s cells with different culture times. Hemolysis tests indicated that the copolymers were hemocompatible, and the presence of copolymers could reduce the hemolysis ratio of DOX significantly. These results suggested that the novel anticancer nanomedicines based on DOX and amphiphilic Y-shaped copolymers were attractive candidates as tumor tissular and intracellular targeting drug delivery systems in vivo, with enhanced stability during circulation and accelerated drug release at the target sites.Keywords: amphiphilic Y-shaped copolymer, anticancer nanomedicine, cellular proliferation inhibition, doxorubicin
format	article
author	Li D Ding JX Tang ZH Sun H Zhuang XL Xu JZ Chen XS
author_facet	Li D Ding JX Tang ZH Sun H Zhuang XL Xu JZ Chen XS
author_sort	Li D
title	In vitro evaluation of anticancer nanomedicines based on doxorubicin and amphiphilic Y-shaped copolymers
title_short	In vitro evaluation of anticancer nanomedicines based on doxorubicin and amphiphilic Y-shaped copolymers
title_full	In vitro evaluation of anticancer nanomedicines based on doxorubicin and amphiphilic Y-shaped copolymers
title_fullStr	In vitro evaluation of anticancer nanomedicines based on doxorubicin and amphiphilic Y-shaped copolymers
title_full_unstemmed	In vitro evaluation of anticancer nanomedicines based on doxorubicin and amphiphilic Y-shaped copolymers
title_sort	in vitro evaluation of anticancer nanomedicines based on doxorubicin and amphiphilic y-shaped copolymers
publisher	Dove Medical Press
publishDate	2012
url	https://doaj.org/article/da38869c5db3439f8d7fc512aa507650
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In vitro evaluation of anticancer nanomedicines based on doxorubicin and amphiphilic Y-shaped copolymers

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