Molecular form and concentration of serum α2-macroglobulin in diabetes

Abstract α2-Macroglobulin is a highly abundant serum protein involved in the development of atherosclerosis and cardiac hypertrophy. However, its circulating molecular form and exact concentrations in human health/diseases are not known. Blue native-polyacrylamide gel electrophoresis of human serum...

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Autores principales:	Sonomi Yoshino, Kazumi Fujimoto, Tesshu Takada, Sayuki Kawamura, Junro Ogawa, Yuji Kamata, Yoshio Kodera, Masayoshi Shichiri
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2019
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/da3897731279429caa67aecf7d6b1bec
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spelling	oai:doaj.org-article:da3897731279429caa67aecf7d6b1bec2021-12-02T15:08:07ZMolecular form and concentration of serum α2-macroglobulin in diabetes10.1038/s41598-019-49144-72045-2322https://doaj.org/article/da3897731279429caa67aecf7d6b1bec2019-09-01T00:00:00Zhttps://doi.org/10.1038/s41598-019-49144-7https://doaj.org/toc/2045-2322Abstract α2-Macroglobulin is a highly abundant serum protein involved in the development of atherosclerosis and cardiac hypertrophy. However, its circulating molecular form and exact concentrations in human health/diseases are not known. Blue native-polyacrylamide gel electrophoresis of human serum was used to confirm the native conformation of α2-macroglobulin. We created an enzyme-linked immunosorbent assay suitable for quantifying its circulating molecular form and undertook a cross-sectional study to measure its serum levels in 248 patients with diabetes mellitus and 59 healthy volunteers. The predominant circulating molecular form of α2-macroglobulin was the tetramer, whereas its dimer was detectable in patients with high serum levels of α2-macroglobulin. The serum α2-macroglobulin concentration was not associated with glycated hemoglobin or any other glycemic variable as evaluated from 48-h continuous glucose monitoring, but showed close correlation with left ventricular posterior wall thickness, carotid artery intima-media thickness, urinary albumin:creatinine ratio (ACR) and brachial–ankle pulse wave velocity (baPWV). Multivariate analysis revealed only the ACR and baPWV to be independent variables influencing serum levels of α2-macroglobulin. Thus, an increased ACR and baPWV are associated with higher serum concentrations of α2-macroglobulin, and the latter may contribute to the mechanism by which albuminuria increases the risk of developing cardiovascular diseases.Sonomi YoshinoKazumi FujimotoTesshu TakadaSayuki KawamuraJunro OgawaYuji KamataYoshio KoderaMasayoshi ShichiriNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 9, Iss 1, Pp 1-10 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Sonomi Yoshino Kazumi Fujimoto Tesshu Takada Sayuki Kawamura Junro Ogawa Yuji Kamata Yoshio Kodera Masayoshi Shichiri Molecular form and concentration of serum α2-macroglobulin in diabetes
description	Abstract α2-Macroglobulin is a highly abundant serum protein involved in the development of atherosclerosis and cardiac hypertrophy. However, its circulating molecular form and exact concentrations in human health/diseases are not known. Blue native-polyacrylamide gel electrophoresis of human serum was used to confirm the native conformation of α2-macroglobulin. We created an enzyme-linked immunosorbent assay suitable for quantifying its circulating molecular form and undertook a cross-sectional study to measure its serum levels in 248 patients with diabetes mellitus and 59 healthy volunteers. The predominant circulating molecular form of α2-macroglobulin was the tetramer, whereas its dimer was detectable in patients with high serum levels of α2-macroglobulin. The serum α2-macroglobulin concentration was not associated with glycated hemoglobin or any other glycemic variable as evaluated from 48-h continuous glucose monitoring, but showed close correlation with left ventricular posterior wall thickness, carotid artery intima-media thickness, urinary albumin:creatinine ratio (ACR) and brachial–ankle pulse wave velocity (baPWV). Multivariate analysis revealed only the ACR and baPWV to be independent variables influencing serum levels of α2-macroglobulin. Thus, an increased ACR and baPWV are associated with higher serum concentrations of α2-macroglobulin, and the latter may contribute to the mechanism by which albuminuria increases the risk of developing cardiovascular diseases.
format	article
author	Sonomi Yoshino Kazumi Fujimoto Tesshu Takada Sayuki Kawamura Junro Ogawa Yuji Kamata Yoshio Kodera Masayoshi Shichiri
author_facet	Sonomi Yoshino Kazumi Fujimoto Tesshu Takada Sayuki Kawamura Junro Ogawa Yuji Kamata Yoshio Kodera Masayoshi Shichiri
author_sort	Sonomi Yoshino
title	Molecular form and concentration of serum α2-macroglobulin in diabetes
title_short	Molecular form and concentration of serum α2-macroglobulin in diabetes
title_full	Molecular form and concentration of serum α2-macroglobulin in diabetes
title_fullStr	Molecular form and concentration of serum α2-macroglobulin in diabetes
title_full_unstemmed	Molecular form and concentration of serum α2-macroglobulin in diabetes
title_sort	molecular form and concentration of serum α2-macroglobulin in diabetes
publisher	Nature Portfolio
publishDate	2019
url	https://doaj.org/article/da3897731279429caa67aecf7d6b1bec
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Molecular form and concentration of serum α2-macroglobulin in diabetes

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