Reduced adhesion of macrophages on anodized titanium with select nanotube surface features

Amancherla Rajyalakshmi1, Batur Ercan2,3, K Balasubramanian1, Thomas J Webster2,31Non-Ferrous Materials Technology Development Centre, Hyderabad, India; 2School of Engineering, 3Department of Orthopedics, Brown University, Providence, RI, USAAbstract: One of the important prerequisites for a success...

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Autores principales:	Balasubramanian K, Webster TJ, Rajyalakshmi A, Ercan B
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2011
Materias:	Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/da3bd15a40d74e8ebedaa53d7cbdbf66
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spelling	oai:doaj.org-article:da3bd15a40d74e8ebedaa53d7cbdbf662021-12-02T02:42:16ZReduced adhesion of macrophages on anodized titanium with select nanotube surface features1176-91141178-2013https://doaj.org/article/da3bd15a40d74e8ebedaa53d7cbdbf662011-08-01T00:00:00Zhttp://www.dovepress.com/reduced-adhesion-of-macrophages-on-anodized-titanium-with-select-nanot-a8130https://doaj.org/toc/1176-9114https://doaj.org/toc/1178-2013Amancherla Rajyalakshmi1, Batur Ercan2,3, K Balasubramanian1, Thomas J Webster2,31Non-Ferrous Materials Technology Development Centre, Hyderabad, India; 2School of Engineering, 3Department of Orthopedics, Brown University, Providence, RI, USAAbstract: One of the important prerequisites for a successful orthopedic implant apart from being osteoconductive is the elicitation of a favorable immune response that does not lead to the rejection of the implant by the host tissue. Anodization is one of the simplest surface modification processes used to create nanotextured and nanotubular features on metal oxides which has been shown to improve bone formation. Anodization of titanium (Ti) leads to the formation of TiO2 nanotubes on the surface, and the presence of these nanotubes mimics the natural nanoscale features of bone, which in turn contributes to improved bone cell attachment, migration, and proliferation. However, inflammatory cell responses on anodized Ti remains to be tested. It is hypothesized that surface roughness and surface feature size on anodized Ti can be carefully manipulated to control immune cell (specifically, macrophages) responses. Here, when Ti samples were anodized at 10 V in the presence of 1% hydrofluoric acid (HF) for 1 minute, nanotextured (nonnanotube) surfaces were created. When anodization of Ti samples was carried out with 1% HF for 10 minutes at 15 V, nanotubes with 40&ndash;50 nm diameters were formed, whereas at 20 V with 1% HF for 10 minutes, nanotubes with 60&ndash;70 nm diameters were formed. In this study, a reduced density of macrophages was observed after 24 hours of culture on nanotextured and nanotubular Ti samples which were anodized at 10, 15, and 20 V, compared with conventional unmodified Ti samples. This in vitro study thus demonstrated a reduced density of macrophages on anodized Ti, thereby providing further evidence of the greater efficacy of anodized Ti for orthopedic applications.Keywords: anodization, titanium implants, TiO2 nanotubeBalasubramanian KWebster TJRajyalakshmi AErcan BDove Medical PressarticleMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2011, Iss default, Pp 1765-1771 (2011)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine (General) R5-920
spellingShingle	Medicine (General) R5-920 Balasubramanian K Webster TJ Rajyalakshmi A Ercan B Reduced adhesion of macrophages on anodized titanium with select nanotube surface features
description	Amancherla Rajyalakshmi1, Batur Ercan2,3, K Balasubramanian1, Thomas J Webster2,31Non-Ferrous Materials Technology Development Centre, Hyderabad, India; 2School of Engineering, 3Department of Orthopedics, Brown University, Providence, RI, USAAbstract: One of the important prerequisites for a successful orthopedic implant apart from being osteoconductive is the elicitation of a favorable immune response that does not lead to the rejection of the implant by the host tissue. Anodization is one of the simplest surface modification processes used to create nanotextured and nanotubular features on metal oxides which has been shown to improve bone formation. Anodization of titanium (Ti) leads to the formation of TiO2 nanotubes on the surface, and the presence of these nanotubes mimics the natural nanoscale features of bone, which in turn contributes to improved bone cell attachment, migration, and proliferation. However, inflammatory cell responses on anodized Ti remains to be tested. It is hypothesized that surface roughness and surface feature size on anodized Ti can be carefully manipulated to control immune cell (specifically, macrophages) responses. Here, when Ti samples were anodized at 10 V in the presence of 1% hydrofluoric acid (HF) for 1 minute, nanotextured (nonnanotube) surfaces were created. When anodization of Ti samples was carried out with 1% HF for 10 minutes at 15 V, nanotubes with 40&ndash;50 nm diameters were formed, whereas at 20 V with 1% HF for 10 minutes, nanotubes with 60&ndash;70 nm diameters were formed. In this study, a reduced density of macrophages was observed after 24 hours of culture on nanotextured and nanotubular Ti samples which were anodized at 10, 15, and 20 V, compared with conventional unmodified Ti samples. This in vitro study thus demonstrated a reduced density of macrophages on anodized Ti, thereby providing further evidence of the greater efficacy of anodized Ti for orthopedic applications.Keywords: anodization, titanium implants, TiO2 nanotube
format	article
author	Balasubramanian K Webster TJ Rajyalakshmi A Ercan B
author_facet	Balasubramanian K Webster TJ Rajyalakshmi A Ercan B
author_sort	Balasubramanian K
title	Reduced adhesion of macrophages on anodized titanium with select nanotube surface features
title_short	Reduced adhesion of macrophages on anodized titanium with select nanotube surface features
title_full	Reduced adhesion of macrophages on anodized titanium with select nanotube surface features
title_fullStr	Reduced adhesion of macrophages on anodized titanium with select nanotube surface features
title_full_unstemmed	Reduced adhesion of macrophages on anodized titanium with select nanotube surface features
title_sort	reduced adhesion of macrophages on anodized titanium with select nanotube surface features
publisher	Dove Medical Press
publishDate	2011
url	https://doaj.org/article/da3bd15a40d74e8ebedaa53d7cbdbf66
work_keys_str_mv	AT balasubramaniank reducedadhesionofmacrophagesonanodizedtitaniumwithselectnanotubesurfacefeatures AT webstertj reducedadhesionofmacrophagesonanodizedtitaniumwithselectnanotubesurfacefeatures AT rajyalakshmia reducedadhesionofmacrophagesonanodizedtitaniumwithselectnanotubesurfacefeatures AT ercanb reducedadhesionofmacrophagesonanodizedtitaniumwithselectnanotubesurfacefeatures
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Reduced adhesion of macrophages on anodized titanium with select nanotube surface features

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