Morphological and genomic evidence for a new species of Corallorhiza (Orchidaceae: Epidendroideae) from SW China

Corallorhiza sinensis, a new species of mycoheterotrophic orchid from western Sichuan, China, is described and illustrated based on molecular and morphological evidence. It is morphologically similar to Corallorhiza trifida, but can be distinguished by bigger flowers, both sepals and petals with 3 v...

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Autores principales:	Jia-Xin Yang, Shuai Peng, Jun-Jie Wang, Shi-Xiong Ding, Yan Wang, Jing Tian, Han Yang, Guang-Wan Hu, Qing-Feng Wang
Formato:	article
Lenguaje:	EN
Publicado:	KeAi Communications Co., Ltd. 2021
Materias:	Corallorhiza sinensis Oreorchis Sichuan province Plastome Phylogeny Biology (General) QH301-705.5 Botany QK1-989
Acceso en línea:	https://doaj.org/article/da3e2640c13242b3a3ca8cd45bc98c89
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spelling	oai:doaj.org-article:da3e2640c13242b3a3ca8cd45bc98c892021-11-12T04:43:49ZMorphological and genomic evidence for a new species of Corallorhiza (Orchidaceae: Epidendroideae) from SW China2468-265910.1016/j.pld.2021.01.002https://doaj.org/article/da3e2640c13242b3a3ca8cd45bc98c892021-10-01T00:00:00Zhttp://www.sciencedirect.com/science/article/pii/S2468265921000020https://doaj.org/toc/2468-2659Corallorhiza sinensis, a new species of mycoheterotrophic orchid from western Sichuan, China, is described and illustrated based on molecular and morphological evidence. It is morphologically similar to Corallorhiza trifida, but can be distinguished by bigger flowers, both sepals and petals with 3 veins, and longer lateral lobes of lip. To distinguish the new Corallorhiza species and explore its phylogenetic position within subtribe Calypsoinae, this study employed sequences of the nuclear ribosomal DNA (nrDNA) and whole plastome assembled from the genome skimming approach. The plastome is 148,124 bp in length, including a pair of inverted repeats (IRs) of 26,165 bp, a large single-copy region (LSC) of 82,207 bp, and a small single-copy region (SSC) of 13,587 bp. Further, phylogenetic analyses were performed using nrDNA sequence and 79 coding sequences (CDSs) from 26 complete plastomes of subtribe Calypsoinae. The phylogenetic tree based on nrDNA sequence suggested that Corallorhiza is a monophyletic group, and strongly support C. sinensis as sister to the rest species of Corallorhiza. The plastid tree showed that 10 Corallorhiza species grouped into two clades and C. sinensis is most closely related to the North American C. striata and C. bentleyi instead of Oreorchis foliosa and O. angustata in the same clade. The plastid tree and nrDNA tree indicate Oreorchis is a paraphyletic. Although the topological conflicts are displayed between plastome and nrDNA phylogenies of C. sinensis, it is still the most closely related to Corallorhiza. Comparative analysis showed that C. sinensis populations are characteristic of the intermediate morphological traits between Corallorhiza and Oreorchis. The finding of this new species from China shed new light on the phylogeny of Oreorchis and Corallorhiza.Jia-Xin YangShuai PengJun-Jie WangShi-Xiong DingYan WangJing TianHan YangGuang-Wan HuQing-Feng WangKeAi Communications Co., Ltd.articleCorallorhiza sinensisOreorchisSichuan provincePlastomePhylogenyBiology (General)QH301-705.5BotanyQK1-989ENPlant Diversity, Vol 43, Iss 5, Pp 409-419 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Corallorhiza sinensis Oreorchis Sichuan province Plastome Phylogeny Biology (General) QH301-705.5 Botany QK1-989
spellingShingle	Corallorhiza sinensis Oreorchis Sichuan province Plastome Phylogeny Biology (General) QH301-705.5 Botany QK1-989 Jia-Xin Yang Shuai Peng Jun-Jie Wang Shi-Xiong Ding Yan Wang Jing Tian Han Yang Guang-Wan Hu Qing-Feng Wang Morphological and genomic evidence for a new species of Corallorhiza (Orchidaceae: Epidendroideae) from SW China
description	Corallorhiza sinensis, a new species of mycoheterotrophic orchid from western Sichuan, China, is described and illustrated based on molecular and morphological evidence. It is morphologically similar to Corallorhiza trifida, but can be distinguished by bigger flowers, both sepals and petals with 3 veins, and longer lateral lobes of lip. To distinguish the new Corallorhiza species and explore its phylogenetic position within subtribe Calypsoinae, this study employed sequences of the nuclear ribosomal DNA (nrDNA) and whole plastome assembled from the genome skimming approach. The plastome is 148,124 bp in length, including a pair of inverted repeats (IRs) of 26,165 bp, a large single-copy region (LSC) of 82,207 bp, and a small single-copy region (SSC) of 13,587 bp. Further, phylogenetic analyses were performed using nrDNA sequence and 79 coding sequences (CDSs) from 26 complete plastomes of subtribe Calypsoinae. The phylogenetic tree based on nrDNA sequence suggested that Corallorhiza is a monophyletic group, and strongly support C. sinensis as sister to the rest species of Corallorhiza. The plastid tree showed that 10 Corallorhiza species grouped into two clades and C. sinensis is most closely related to the North American C. striata and C. bentleyi instead of Oreorchis foliosa and O. angustata in the same clade. The plastid tree and nrDNA tree indicate Oreorchis is a paraphyletic. Although the topological conflicts are displayed between plastome and nrDNA phylogenies of C. sinensis, it is still the most closely related to Corallorhiza. Comparative analysis showed that C. sinensis populations are characteristic of the intermediate morphological traits between Corallorhiza and Oreorchis. The finding of this new species from China shed new light on the phylogeny of Oreorchis and Corallorhiza.
format	article
author	Jia-Xin Yang Shuai Peng Jun-Jie Wang Shi-Xiong Ding Yan Wang Jing Tian Han Yang Guang-Wan Hu Qing-Feng Wang
author_facet	Jia-Xin Yang Shuai Peng Jun-Jie Wang Shi-Xiong Ding Yan Wang Jing Tian Han Yang Guang-Wan Hu Qing-Feng Wang
author_sort	Jia-Xin Yang
title	Morphological and genomic evidence for a new species of Corallorhiza (Orchidaceae: Epidendroideae) from SW China
title_short	Morphological and genomic evidence for a new species of Corallorhiza (Orchidaceae: Epidendroideae) from SW China
title_full	Morphological and genomic evidence for a new species of Corallorhiza (Orchidaceae: Epidendroideae) from SW China
title_fullStr	Morphological and genomic evidence for a new species of Corallorhiza (Orchidaceae: Epidendroideae) from SW China
title_full_unstemmed	Morphological and genomic evidence for a new species of Corallorhiza (Orchidaceae: Epidendroideae) from SW China
title_sort	morphological and genomic evidence for a new species of corallorhiza (orchidaceae: epidendroideae) from sw china
publisher	KeAi Communications Co., Ltd.
publishDate	2021
url	https://doaj.org/article/da3e2640c13242b3a3ca8cd45bc98c89
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Morphological and genomic evidence for a new species of Corallorhiza (Orchidaceae: Epidendroideae) from SW China

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