Clinical characteristics and viral load dynamics of COVID-19 in a mildly or moderately symptomatic outpatient sample.

<h4>Background</h4>Studies of outpatients with mild or moderate COVID-19 are uncommon. We studied: 1) association of symptoms with reverse transcriptase polymerase chain reaction (RT-PCR) test results; and 2) association of initial RT-PCR cycle threshold (Ct) in relation to duration of R...

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Autores principales:	Amanda Caplan, Kelly W Bates, Carla Brioni, Aileen Santos, Linda M Sabatini, Karen L Kaul, Mercedes R Carnethon, Janardan D Khandekar, Philip Greenland
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/da47636864cf446fa0966d8282df913c
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spelling	oai:doaj.org-article:da47636864cf446fa0966d8282df913c2021-12-02T20:07:48ZClinical characteristics and viral load dynamics of COVID-19 in a mildly or moderately symptomatic outpatient sample.1932-620310.1371/journal.pone.0258970https://doaj.org/article/da47636864cf446fa0966d8282df913c2021-01-01T00:00:00Zhttps://doi.org/10.1371/journal.pone.0258970https://doaj.org/toc/1932-6203<h4>Background</h4>Studies of outpatients with mild or moderate COVID-19 are uncommon. We studied: 1) association of symptoms with reverse transcriptase polymerase chain reaction (RT-PCR) test results; and 2) association of initial RT-PCR cycle threshold (Ct) in relation to duration of RT-PCR positivity in outpatients with mild or moderate COVID-19.<h4>Methods</h4>This was a cohort study of outpatients with confirmed COVID-19 and at least one symptom. Participants had repeat nasopharyngeal swabs and symptom checklists every 3-5 days until two consecutive RT-PCR tests were negative. RT-PCR tests were used to assess viral load. Antibody tests for COVID-19 were performed at 2 weeks, 4 weeks, and 8 weeks after symptom onset.<h4>Results</h4>Twenty-five patients (nine females) were enrolled, ranging in age from 19-58 (median age 28 years). All patients reported at least one symptom, with a median of six symptoms per patient. Symptoms persisted for 6-67 days (median duration 18 days). In all 25 patients, blood samples collected a median of 13 days after symptom onset were positive for SARS-CoV-2 antibodies in 15 (60%). After a median of 28 days following symptom onset, 23/23 patients with available samples tested positive for antibodies. The longest duration of positive RT-PCR test was 49 days from first positive PCR test (Mean = 27.4, SD = 12.5, Median = 24). Initial Ct was significantly associated with longer duration (β = -1.3, SE = 0.3, p<0.01 per 1 cycle higher) of RT-PCR positivity.<h4>Conclusions</h4>In mildly or moderately ill COVID-19 outpatients, RT-PCT tests remained positive for as long as 49 days and test positivity and symptom duration correlated with initial viral load.Amanda CaplanKelly W BatesCarla BrioniAileen SantosLinda M SabatiniKaren L KaulMercedes R CarnethonJanardan D KhandekarPhilip GreenlandPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 16, Iss 10, p e0258970 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Amanda Caplan Kelly W Bates Carla Brioni Aileen Santos Linda M Sabatini Karen L Kaul Mercedes R Carnethon Janardan D Khandekar Philip Greenland Clinical characteristics and viral load dynamics of COVID-19 in a mildly or moderately symptomatic outpatient sample.
description	<h4>Background</h4>Studies of outpatients with mild or moderate COVID-19 are uncommon. We studied: 1) association of symptoms with reverse transcriptase polymerase chain reaction (RT-PCR) test results; and 2) association of initial RT-PCR cycle threshold (Ct) in relation to duration of RT-PCR positivity in outpatients with mild or moderate COVID-19.<h4>Methods</h4>This was a cohort study of outpatients with confirmed COVID-19 and at least one symptom. Participants had repeat nasopharyngeal swabs and symptom checklists every 3-5 days until two consecutive RT-PCR tests were negative. RT-PCR tests were used to assess viral load. Antibody tests for COVID-19 were performed at 2 weeks, 4 weeks, and 8 weeks after symptom onset.<h4>Results</h4>Twenty-five patients (nine females) were enrolled, ranging in age from 19-58 (median age 28 years). All patients reported at least one symptom, with a median of six symptoms per patient. Symptoms persisted for 6-67 days (median duration 18 days). In all 25 patients, blood samples collected a median of 13 days after symptom onset were positive for SARS-CoV-2 antibodies in 15 (60%). After a median of 28 days following symptom onset, 23/23 patients with available samples tested positive for antibodies. The longest duration of positive RT-PCR test was 49 days from first positive PCR test (Mean = 27.4, SD = 12.5, Median = 24). Initial Ct was significantly associated with longer duration (β = -1.3, SE = 0.3, p<0.01 per 1 cycle higher) of RT-PCR positivity.<h4>Conclusions</h4>In mildly or moderately ill COVID-19 outpatients, RT-PCT tests remained positive for as long as 49 days and test positivity and symptom duration correlated with initial viral load.
format	article
author	Amanda Caplan Kelly W Bates Carla Brioni Aileen Santos Linda M Sabatini Karen L Kaul Mercedes R Carnethon Janardan D Khandekar Philip Greenland
author_facet	Amanda Caplan Kelly W Bates Carla Brioni Aileen Santos Linda M Sabatini Karen L Kaul Mercedes R Carnethon Janardan D Khandekar Philip Greenland
author_sort	Amanda Caplan
title	Clinical characteristics and viral load dynamics of COVID-19 in a mildly or moderately symptomatic outpatient sample.
title_short	Clinical characteristics and viral load dynamics of COVID-19 in a mildly or moderately symptomatic outpatient sample.
title_full	Clinical characteristics and viral load dynamics of COVID-19 in a mildly or moderately symptomatic outpatient sample.
title_fullStr	Clinical characteristics and viral load dynamics of COVID-19 in a mildly or moderately symptomatic outpatient sample.
title_full_unstemmed	Clinical characteristics and viral load dynamics of COVID-19 in a mildly or moderately symptomatic outpatient sample.
title_sort	clinical characteristics and viral load dynamics of covid-19 in a mildly or moderately symptomatic outpatient sample.
publisher	Public Library of Science (PLoS)
publishDate	2021
url	https://doaj.org/article/da47636864cf446fa0966d8282df913c
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Clinical characteristics and viral load dynamics of COVID-19 in a mildly or moderately symptomatic outpatient sample.

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