Tissue-engineered trachea from a 3D-printed scaffold enhances whole-segment tracheal repair

Abstract Long segmental repair of trachea stenosis is an intractable condition in the clinic. The reconstruction of an artificial substitute by tissue engineering is a promising approach to solve this unmet clinical need. 3D printing technology provides an infinite possibility for engineering a trac...

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Autores principales:	Manchen Gao, Hengyi Zhang, Wei Dong, Jie Bai, Botao Gao, Dekai Xia, Bei Feng, Maolin Chen, Xiaomin He, Meng Yin, Zhiwei Xu, Nevin Witman, Wei Fu, Jinghao Zheng
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/da48b5d74b38408ea439db692a88d3aa
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spelling	oai:doaj.org-article:da48b5d74b38408ea439db692a88d3aa2021-12-02T15:05:31ZTissue-engineered trachea from a 3D-printed scaffold enhances whole-segment tracheal repair10.1038/s41598-017-05518-32045-2322https://doaj.org/article/da48b5d74b38408ea439db692a88d3aa2017-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-05518-3https://doaj.org/toc/2045-2322Abstract Long segmental repair of trachea stenosis is an intractable condition in the clinic. The reconstruction of an artificial substitute by tissue engineering is a promising approach to solve this unmet clinical need. 3D printing technology provides an infinite possibility for engineering a trachea. Here, we 3D printed a biodegradable reticular polycaprolactone (PCL) scaffold with similar morphology to the whole segment of rabbits’ native trachea. The 3D-printed scaffold was suspended in culture with chondrocytes for 2 (Group I) or 4 (Group II) weeks, respectively. This in vitro suspension produced a more successful reconstruction of a tissue-engineered trachea (TET), which enhanced the overall support function of the replaced tracheal segment. After implantation of the chondrocyte-treated scaffold into the subcutaneous tissue of nude mice, the TET presented properties of mature cartilage tissue. To further evaluate the feasibility of repairing whole segment tracheal defects, replacement surgery of rabbits’ native trachea by TET was performed. Following postoperative care, mean survival time in Group I was 14.38 ± 5.42 days, and in Group II was 22.58 ± 16.10 days, with the longest survival time being 10 weeks in Group II. In conclusion, we demonstrate the feasibility of repairing whole segment tracheal defects with 3D printed TET.Manchen GaoHengyi ZhangWei DongJie BaiBotao GaoDekai XiaBei FengMaolin ChenXiaomin HeMeng YinZhiwei XuNevin WitmanWei FuJinghao ZhengNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-12 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Manchen Gao Hengyi Zhang Wei Dong Jie Bai Botao Gao Dekai Xia Bei Feng Maolin Chen Xiaomin He Meng Yin Zhiwei Xu Nevin Witman Wei Fu Jinghao Zheng Tissue-engineered trachea from a 3D-printed scaffold enhances whole-segment tracheal repair
description	Abstract Long segmental repair of trachea stenosis is an intractable condition in the clinic. The reconstruction of an artificial substitute by tissue engineering is a promising approach to solve this unmet clinical need. 3D printing technology provides an infinite possibility for engineering a trachea. Here, we 3D printed a biodegradable reticular polycaprolactone (PCL) scaffold with similar morphology to the whole segment of rabbits’ native trachea. The 3D-printed scaffold was suspended in culture with chondrocytes for 2 (Group I) or 4 (Group II) weeks, respectively. This in vitro suspension produced a more successful reconstruction of a tissue-engineered trachea (TET), which enhanced the overall support function of the replaced tracheal segment. After implantation of the chondrocyte-treated scaffold into the subcutaneous tissue of nude mice, the TET presented properties of mature cartilage tissue. To further evaluate the feasibility of repairing whole segment tracheal defects, replacement surgery of rabbits’ native trachea by TET was performed. Following postoperative care, mean survival time in Group I was 14.38 ± 5.42 days, and in Group II was 22.58 ± 16.10 days, with the longest survival time being 10 weeks in Group II. In conclusion, we demonstrate the feasibility of repairing whole segment tracheal defects with 3D printed TET.
format	article
author	Manchen Gao Hengyi Zhang Wei Dong Jie Bai Botao Gao Dekai Xia Bei Feng Maolin Chen Xiaomin He Meng Yin Zhiwei Xu Nevin Witman Wei Fu Jinghao Zheng
author_facet	Manchen Gao Hengyi Zhang Wei Dong Jie Bai Botao Gao Dekai Xia Bei Feng Maolin Chen Xiaomin He Meng Yin Zhiwei Xu Nevin Witman Wei Fu Jinghao Zheng
author_sort	Manchen Gao
title	Tissue-engineered trachea from a 3D-printed scaffold enhances whole-segment tracheal repair
title_short	Tissue-engineered trachea from a 3D-printed scaffold enhances whole-segment tracheal repair
title_full	Tissue-engineered trachea from a 3D-printed scaffold enhances whole-segment tracheal repair
title_fullStr	Tissue-engineered trachea from a 3D-printed scaffold enhances whole-segment tracheal repair
title_full_unstemmed	Tissue-engineered trachea from a 3D-printed scaffold enhances whole-segment tracheal repair
title_sort	tissue-engineered trachea from a 3d-printed scaffold enhances whole-segment tracheal repair
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/da48b5d74b38408ea439db692a88d3aa
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Tissue-engineered trachea from a 3D-printed scaffold enhances whole-segment tracheal repair

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