Preparation and in vitro antitumor effects of cytosine arabinoside-loaded genipin-poly-L-glutamic acid-modified bacterial magnetosomes
Yuan-Gang Liu,1,2 Qing-Lei Dai,1 Shi-Bin Wang,1,2 Qiong-Jia Deng,1 Wen-Guo Wu,1,2 Ai-Zheng Chen1,2 1College of Chemical Engineering, 2Institute of Pharmaceutical Engineering, Huaqiao University, Xiamen, People’s Republic of China Abstract: To solve the problem of synthesized magnetic na...
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| Autores principales: | , , , , , |
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| Formato: | article |
| Lenguaje: | EN |
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Dove Medical Press
2015
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| Materias: | |
| Acceso en línea: | https://doaj.org/article/da4d46dd60864a63a7bab78920437dcf |
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| Sumario: | Yuan-Gang Liu,1,2 Qing-Lei Dai,1 Shi-Bin Wang,1,2 Qiong-Jia Deng,1 Wen-Guo Wu,1,2 Ai-Zheng Chen1,2 1College of Chemical Engineering, 2Institute of Pharmaceutical Engineering, Huaqiao University, Xiamen, People’s Republic of China Abstract: To solve the problem of synthesized magnetic nanoparticles in cancer therapy, a new drug delivery system synthesized from bacteria was used to load cytosine arabinoside (Ara-C). Genipin (GP) and poly-l-glutamic acid (PLGA) were selected as dual cross-linkers. The preparation and characterization of Ara-C-loaded GP-PLGA-modified bacterial magnetosomes (BMs) (ABMs-P), as well as their in vitro antitumor effects, were all investigated. Transmission electron micrographs (TEM) and Fourier transform infrared (FTIR) spectroscopy suggested that Ara-C could be bound to the membrane of BMs modified by GP-PLGA. The diameters of the BMs and ABMs-P were 42.0±8.6 nm and 74.9±8.2 nm, respectively. The zeta potential revealed that the nanoparticles were stable. Moreover, this system exhibited optimal drug-loading properties and long-term release behavior. The optimal encapsulation efficiency and drug-loading were 64.1%±6.6% and 38.9%±2.4%, respectively, and ABMs-P could effectively release 90% Ara-C within 40 days, without the release of an initial burst. In addition, in vitro antitumor experiments elucidated that ABMs-P is cytotoxic to HL-60 cell lines, with an inhibition rate of 95%. The method of coupling drugs on BMs using dual cross-linkers is effective, and our results reveal that this new system has potential applications for drug delivery in the future. Keywords: magnetosomes, dual cross-linkers, nanoparticle, drug delivery |
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