Preparation and in vitro antitumor effects of cytosine arabinoside-loaded genipin-poly-L-glutamic acid-modified bacterial magnetosomes

Preparation and in vitro antitumor effects of cytosine arabinoside-loaded genipin-poly-L-glutamic acid-modified bacterial magnetosomes

Yuan-Gang Liu,1,2 Qing-Lei Dai,1 Shi-Bin Wang,1,2 Qiong-Jia Deng,1 Wen-Guo Wu,1,2 Ai-Zheng Chen1,2 1College of Chemical Engineering, 2Institute of Pharmaceutical Engineering, Huaqiao University, Xiamen, People’s Republic of China Abstract: To solve the problem of synthesized magnetic na...

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Autores principales: Liu YG, Dai QL, Wang SB, Deng QJ, Wu WG, Chen AZ
Formato: article
Lenguaje:EN
Publicado: Dove Medical Press 2015
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Medicine (General)
R5-920
Acceso en línea:https://doaj.org/article/da4d46dd60864a63a7bab78920437dcf
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spelling oai:doaj.org-article:da4d46dd60864a63a7bab78920437dcf2021-12-02T05:40:37ZPreparation and in vitro antitumor effects of cytosine arabinoside-loaded genipin-poly-L-glutamic acid-modified bacterial magnetosomes1178-2013https://doaj.org/article/da4d46dd60864a63a7bab78920437dcf2015-02-01T00:00:00Zhttp://www.dovepress.com/preparation-and-in-vitro-antitumor-effects-ofnbspcytosine-arabinoside--peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013 Yuan-Gang Liu,1,2 Qing-Lei Dai,1 Shi-Bin Wang,1,2 Qiong-Jia Deng,1 Wen-Guo Wu,1,2 Ai-Zheng Chen1,2 1College of Chemical Engineering, 2Institute of Pharmaceutical Engineering, Huaqiao University, Xiamen, People’s Republic of China Abstract: To solve the problem of synthesized magnetic nanoparticles in cancer therapy, a new drug delivery system synthesized from bacteria was used to load cytosine arabinoside (Ara-C). Genipin (GP) and poly-l-glutamic acid (PLGA) were selected as dual cross-linkers. The preparation and characterization of Ara-C-loaded GP-PLGA-modified bacterial magnetosomes (BMs) (ABMs-P), as well as their in vitro antitumor effects, were all investigated. Transmission electron micrographs (TEM) and Fourier transform infrared (FTIR) spectroscopy suggested that Ara-C could be bound to the membrane of BMs modified by GP-PLGA. The diameters of the BMs and ABMs-P were 42.0±8.6 nm and 74.9±8.2 nm, respectively. The zeta potential revealed that the nanoparticles were stable. Moreover, this system exhibited optimal drug-loading properties and long-term release behavior. The optimal encapsulation efficiency and drug-loading were 64.1%±6.6% and 38.9%±2.4%, respectively, and ABMs-P could effectively release 90% Ara-C within 40 days, without the release of an initial burst. In addition, in vitro antitumor experiments elucidated that ABMs-P is cytotoxic to HL-60 cell lines, with an inhibition rate of 95%. The method of coupling drugs on BMs using dual cross-linkers is effective, and our results reveal that this new system has potential applications for drug delivery in the future. Keywords: magnetosomes, dual cross-linkers, nanoparticle, drug deliveryLiu YGDai QLWang SBDeng QJWu WGChen AZDove Medical PressarticleMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2015, Iss default, Pp 1387-1397 (2015)
institution DOAJ
collection DOAJ
language EN
topic Medicine (General)
R5-920
spellingShingle Medicine (General)
R5-920
Liu YG
Dai QL
Wang SB
Deng QJ
Wu WG
Chen AZ
Preparation and in vitro antitumor effects of cytosine arabinoside-loaded genipin-poly-L-glutamic acid-modified bacterial magnetosomes
description Yuan-Gang Liu,1,2 Qing-Lei Dai,1 Shi-Bin Wang,1,2 Qiong-Jia Deng,1 Wen-Guo Wu,1,2 Ai-Zheng Chen1,2 1College of Chemical Engineering, 2Institute of Pharmaceutical Engineering, Huaqiao University, Xiamen, People’s Republic of China Abstract: To solve the problem of synthesized magnetic nanoparticles in cancer therapy, a new drug delivery system synthesized from bacteria was used to load cytosine arabinoside (Ara-C). Genipin (GP) and poly-l-glutamic acid (PLGA) were selected as dual cross-linkers. The preparation and characterization of Ara-C-loaded GP-PLGA-modified bacterial magnetosomes (BMs) (ABMs-P), as well as their in vitro antitumor effects, were all investigated. Transmission electron micrographs (TEM) and Fourier transform infrared (FTIR) spectroscopy suggested that Ara-C could be bound to the membrane of BMs modified by GP-PLGA. The diameters of the BMs and ABMs-P were 42.0±8.6 nm and 74.9±8.2 nm, respectively. The zeta potential revealed that the nanoparticles were stable. Moreover, this system exhibited optimal drug-loading properties and long-term release behavior. The optimal encapsulation efficiency and drug-loading were 64.1%±6.6% and 38.9%±2.4%, respectively, and ABMs-P could effectively release 90% Ara-C within 40 days, without the release of an initial burst. In addition, in vitro antitumor experiments elucidated that ABMs-P is cytotoxic to HL-60 cell lines, with an inhibition rate of 95%. The method of coupling drugs on BMs using dual cross-linkers is effective, and our results reveal that this new system has potential applications for drug delivery in the future. Keywords: magnetosomes, dual cross-linkers, nanoparticle, drug delivery
format article
author Liu YG
Dai QL
Wang SB
Deng QJ
Wu WG
Chen AZ
author_facet Liu YG
Dai QL
Wang SB
Deng QJ
Wu WG
Chen AZ
author_sort Liu YG
title Preparation and in vitro antitumor effects of cytosine arabinoside-loaded genipin-poly-L-glutamic acid-modified bacterial magnetosomes
title_short Preparation and in vitro antitumor effects of cytosine arabinoside-loaded genipin-poly-L-glutamic acid-modified bacterial magnetosomes
title_full Preparation and in vitro antitumor effects of cytosine arabinoside-loaded genipin-poly-L-glutamic acid-modified bacterial magnetosomes
title_fullStr Preparation and in vitro antitumor effects of cytosine arabinoside-loaded genipin-poly-L-glutamic acid-modified bacterial magnetosomes
title_full_unstemmed Preparation and in vitro antitumor effects of cytosine arabinoside-loaded genipin-poly-L-glutamic acid-modified bacterial magnetosomes
title_sort preparation and in vitro antitumor effects of cytosine arabinoside-loaded genipin-poly-l-glutamic acid-modified bacterial magnetosomes
publisher Dove Medical Press
publishDate 2015
url https://doaj.org/article/da4d46dd60864a63a7bab78920437dcf
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