Development of IC-ELISA and immunochromatographic strip assay for the detection of flunixin meglumine in milk
Flunixin meglumine (FM) is a novel nonsteroidal anti-inflammatory drug for animals, which has antipyretic, analgesic, and anti-inflammatory effects. The drug, which was originally used to relieve inflammation in horses, musculoskeletal disorders, and pain, has been approved for use in endotoxemia, i...
Guardado en:
| Autores principales: | , , , , , |
|---|---|
| Formato: | article |
| Lenguaje: | EN |
| Publicado: |
Taylor & Francis Group
2018
|
| Materias: | |
| Acceso en línea: | https://doaj.org/article/da5229dac09244b2a524f1c45f10dc0d |
| Etiquetas: |
Agregar Etiqueta
Sin Etiquetas, Sea el primero en etiquetar este registro!
|
| id |
oai:doaj.org-article:da5229dac09244b2a524f1c45f10dc0d |
|---|---|
| record_format |
dspace |
| spelling |
oai:doaj.org-article:da5229dac09244b2a524f1c45f10dc0d2021-11-26T11:19:46ZDevelopment of IC-ELISA and immunochromatographic strip assay for the detection of flunixin meglumine in milk0954-01051465-344310.1080/09540105.2017.1364710https://doaj.org/article/da5229dac09244b2a524f1c45f10dc0d2018-01-01T00:00:00Zhttp://dx.doi.org/10.1080/09540105.2017.1364710https://doaj.org/toc/0954-0105https://doaj.org/toc/1465-3443Flunixin meglumine (FM) is a novel nonsteroidal anti-inflammatory drug for animals, which has antipyretic, analgesic, and anti-inflammatory effects. The drug, which was originally used to relieve inflammation in horses, musculoskeletal disorders, and pain, has been approved for use in endotoxemia, infectious diseases in swine, etc. A sensitive anti-FM monoclonal antibody 2H4 was prepared and used to develop an indirect competitive enzyme-linked immunosorbent assay and immunochromatographic strip assay for the detection of FM in milk. The complete antigen and coating antigen were conjugated with bovine serum albumin and ovalbumin, respectively. The monoclonal antibody 2H4, with a half inhibition concentration of 0.29 ng/mL, had a limit of detection of 0.432 ng/mL and a linear range of detection of 0.08664–0.97226 ng/mL. A sensitive and convenient immunochromatographic strip assay was developed with an FM cutoff value of 0.29 ng/mL. The developed methods were suitable for the detection of FM in milk.Lu LinWei JiangLiguang XuLiqiang LiuShanshan SongHua KuangTaylor & Francis Grouparticleflunixin megluminemonoclonal antibodyimmunochromatographic strip assayic-elisamilkAgriculture (General)S1-972Immunologic diseases. AllergyRC581-607ENFood and Agricultural Immunology, Vol 29, Iss 1, Pp 193-203 (2018) |
| institution |
DOAJ |
| collection |
DOAJ |
| language |
EN |
| topic |
flunixin meglumine monoclonal antibody immunochromatographic strip assay ic-elisa milk Agriculture (General) S1-972 Immunologic diseases. Allergy RC581-607 |
| spellingShingle |
flunixin meglumine monoclonal antibody immunochromatographic strip assay ic-elisa milk Agriculture (General) S1-972 Immunologic diseases. Allergy RC581-607 Lu Lin Wei Jiang Liguang Xu Liqiang Liu Shanshan Song Hua Kuang Development of IC-ELISA and immunochromatographic strip assay for the detection of flunixin meglumine in milk |
| description |
Flunixin meglumine (FM) is a novel nonsteroidal anti-inflammatory drug for animals, which has antipyretic, analgesic, and anti-inflammatory effects. The drug, which was originally used to relieve inflammation in horses, musculoskeletal disorders, and pain, has been approved for use in endotoxemia, infectious diseases in swine, etc. A sensitive anti-FM monoclonal antibody 2H4 was prepared and used to develop an indirect competitive enzyme-linked immunosorbent assay and immunochromatographic strip assay for the detection of FM in milk. The complete antigen and coating antigen were conjugated with bovine serum albumin and ovalbumin, respectively. The monoclonal antibody 2H4, with a half inhibition concentration of 0.29 ng/mL, had a limit of detection of 0.432 ng/mL and a linear range of detection of 0.08664–0.97226 ng/mL. A sensitive and convenient immunochromatographic strip assay was developed with an FM cutoff value of 0.29 ng/mL. The developed methods were suitable for the detection of FM in milk. |
| format |
article |
| author |
Lu Lin Wei Jiang Liguang Xu Liqiang Liu Shanshan Song Hua Kuang |
| author_facet |
Lu Lin Wei Jiang Liguang Xu Liqiang Liu Shanshan Song Hua Kuang |
| author_sort |
Lu Lin |
| title |
Development of IC-ELISA and immunochromatographic strip assay for the detection of flunixin meglumine in milk |
| title_short |
Development of IC-ELISA and immunochromatographic strip assay for the detection of flunixin meglumine in milk |
| title_full |
Development of IC-ELISA and immunochromatographic strip assay for the detection of flunixin meglumine in milk |
| title_fullStr |
Development of IC-ELISA and immunochromatographic strip assay for the detection of flunixin meglumine in milk |
| title_full_unstemmed |
Development of IC-ELISA and immunochromatographic strip assay for the detection of flunixin meglumine in milk |
| title_sort |
development of ic-elisa and immunochromatographic strip assay for the detection of flunixin meglumine in milk |
| publisher |
Taylor & Francis Group |
| publishDate |
2018 |
| url |
https://doaj.org/article/da5229dac09244b2a524f1c45f10dc0d |
| work_keys_str_mv |
AT lulin developmentoficelisaandimmunochromatographicstripassayforthedetectionofflunixinmeglumineinmilk AT weijiang developmentoficelisaandimmunochromatographicstripassayforthedetectionofflunixinmeglumineinmilk AT liguangxu developmentoficelisaandimmunochromatographicstripassayforthedetectionofflunixinmeglumineinmilk AT liqiangliu developmentoficelisaandimmunochromatographicstripassayforthedetectionofflunixinmeglumineinmilk AT shanshansong developmentoficelisaandimmunochromatographicstripassayforthedetectionofflunixinmeglumineinmilk AT huakuang developmentoficelisaandimmunochromatographicstripassayforthedetectionofflunixinmeglumineinmilk |
| _version_ |
1718409564638937088 |