Aging differentially affects multiple aspects of vesicle fusion kinetics.

How fusion pore formation during exocytosis affects the subsequent release of vesicle contents remains incompletely understood. It is unclear if the amount released per vesicle is dependent upon the nature of the developing fusion pore and whether full fusion and transient kiss and run exocytosis ar...

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Autores principales:	Mark P Zanin, Lucy Phillips, Kimberly D Mackenzie, Damien J Keating
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2011
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/da5ec368697040379fdfbf23a81343fa
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spelling	oai:doaj.org-article:da5ec368697040379fdfbf23a81343fa2021-11-18T07:33:53ZAging differentially affects multiple aspects of vesicle fusion kinetics.1932-620310.1371/journal.pone.0027820https://doaj.org/article/da5ec368697040379fdfbf23a81343fa2011-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/22125627/?tool=EBIhttps://doaj.org/toc/1932-6203How fusion pore formation during exocytosis affects the subsequent release of vesicle contents remains incompletely understood. It is unclear if the amount released per vesicle is dependent upon the nature of the developing fusion pore and whether full fusion and transient kiss and run exocytosis are regulated by similar mechanisms. We hypothesise that if consistent relationships exist between these aspects of exocytosis then they will remain constant across any age. Using amperometry in mouse chromaffin cells we measured catecholamine efflux during single exocytotic events at P0, 1 month and 6 months. At all ages we observed full fusion (amperometric spike only), full fusion preceded by fusion pore flickering (pre-spike foot (PSF) signal followed by a spike) and pure "kiss and run" exocytosis (represented by stand alone foot (SAF) signals). We observe age-associated increases in the size of all 3 modes of fusion but these increases occur at different ages. The release probability of PSF signals or full spikes alone doesn't alter across any age in comparison with an age-dependent increase in the incidence of "kiss and run" type events. However, the most striking changes we observe are age-associated changes in the relationship between vesicle size and the membrane bending energy required for exocytosis. Our data illustrates that vesicle size does not regulate release probability, as has been suggested, that membrane elasticity or flexural rigidity change with age and that the mechanisms controlling full fusion may differ from those controlling "kiss and run" fusion.Mark P ZaninLucy PhillipsKimberly D MackenzieDamien J KeatingPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 6, Iss 11, p e27820 (2011)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Mark P Zanin Lucy Phillips Kimberly D Mackenzie Damien J Keating Aging differentially affects multiple aspects of vesicle fusion kinetics.
description	How fusion pore formation during exocytosis affects the subsequent release of vesicle contents remains incompletely understood. It is unclear if the amount released per vesicle is dependent upon the nature of the developing fusion pore and whether full fusion and transient kiss and run exocytosis are regulated by similar mechanisms. We hypothesise that if consistent relationships exist between these aspects of exocytosis then they will remain constant across any age. Using amperometry in mouse chromaffin cells we measured catecholamine efflux during single exocytotic events at P0, 1 month and 6 months. At all ages we observed full fusion (amperometric spike only), full fusion preceded by fusion pore flickering (pre-spike foot (PSF) signal followed by a spike) and pure "kiss and run" exocytosis (represented by stand alone foot (SAF) signals). We observe age-associated increases in the size of all 3 modes of fusion but these increases occur at different ages. The release probability of PSF signals or full spikes alone doesn't alter across any age in comparison with an age-dependent increase in the incidence of "kiss and run" type events. However, the most striking changes we observe are age-associated changes in the relationship between vesicle size and the membrane bending energy required for exocytosis. Our data illustrates that vesicle size does not regulate release probability, as has been suggested, that membrane elasticity or flexural rigidity change with age and that the mechanisms controlling full fusion may differ from those controlling "kiss and run" fusion.
format	article
author	Mark P Zanin Lucy Phillips Kimberly D Mackenzie Damien J Keating
author_facet	Mark P Zanin Lucy Phillips Kimberly D Mackenzie Damien J Keating
author_sort	Mark P Zanin
title	Aging differentially affects multiple aspects of vesicle fusion kinetics.
title_short	Aging differentially affects multiple aspects of vesicle fusion kinetics.
title_full	Aging differentially affects multiple aspects of vesicle fusion kinetics.
title_fullStr	Aging differentially affects multiple aspects of vesicle fusion kinetics.
title_full_unstemmed	Aging differentially affects multiple aspects of vesicle fusion kinetics.
title_sort	aging differentially affects multiple aspects of vesicle fusion kinetics.
publisher	Public Library of Science (PLoS)
publishDate	2011
url	https://doaj.org/article/da5ec368697040379fdfbf23a81343fa
work_keys_str_mv	AT markpzanin agingdifferentiallyaffectsmultipleaspectsofvesiclefusionkinetics AT lucyphillips agingdifferentiallyaffectsmultipleaspectsofvesiclefusionkinetics AT kimberlydmackenzie agingdifferentiallyaffectsmultipleaspectsofvesiclefusionkinetics AT damienjkeating agingdifferentiallyaffectsmultipleaspectsofvesiclefusionkinetics
_version_	1718423317244805120

Aging differentially affects multiple aspects of vesicle fusion kinetics.

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