Oxygen and indocyanine green loaded phase-transition nanoparticle-mediated photo-sonodynamic cytotoxic effects on rheumatoid arthritis fibroblast-like synoviocytes

Qin Tang,1–3 Jianyu Cui,1 Zhonghua Tian,1 Jiangchuan Sun,4 Zhigang Wang,2 Shufang Chang,4 Shenyin Zhu1 1Department of Pharmacy, First Affiliated Hospital of Chongqing Medical University, 2Department of Ultrasound, Institute of Ultrasound Imaging, Second Affiliated Hospital of Chongqing Me...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Tang Q, Cui J, Tian Z, Sun J, Wang Z, Chang S, Zhu S
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2017
Materias:	multifunctional nanoparticles indocyanine green synoviocytes reactive oxygen species apoptosis Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/da6a46a322064e60b29e31001fd364fe
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:da6a46a322064e60b29e31001fd364fe
record_format	dspace
spelling	oai:doaj.org-article:da6a46a322064e60b29e31001fd364fe2021-12-02T01:50:38ZOxygen and indocyanine green loaded phase-transition nanoparticle-mediated photo-sonodynamic cytotoxic effects on rheumatoid arthritis fibroblast-like synoviocytes1178-2013https://doaj.org/article/da6a46a322064e60b29e31001fd364fe2017-01-01T00:00:00Zhttps://www.dovepress.com/oxygen-and-indocyanine-green-loaded-phase-transition-nanoparticle-medi-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Qin Tang,1–3 Jianyu Cui,1 Zhonghua Tian,1 Jiangchuan Sun,4 Zhigang Wang,2 Shufang Chang,4 Shenyin Zhu1 1Department of Pharmacy, First Affiliated Hospital of Chongqing Medical University, 2Department of Ultrasound, Institute of Ultrasound Imaging, Second Affiliated Hospital of Chongqing Medical University, 3State Key Laboratory of Ultrasound Engineering in Medicine Co-founded by Chongqing and the Ministry of Science and Technology, 4Department of Obstetrics and Gynecology, Second Affiliated Hospital of Chongqing Medical University, Chongqing, People’s Republic of China Background: Photodynamic therapy and sonodynamic therapy are developing, minimally invasive, and site-specific modalities for cancer therapy. A combined strategy PSDT (photodynamic therapy followed by sonodynamic therapy) has been proposed in this study. Here, we aimed to develop novel biodegradable poly(dl-lactide-co-glycolic acid) phase-transition nanoparticles simultaneously loaded with oxygen and indocyanine green (OI-NPs) and to investigate the cytotoxic effects and the potential mechanisms of OI-NP–mediated PSDT on MH7A synoviocytes.Methods: The OI-NPs were prepared using a modified double emulsion method and the physicochemical properties were determined. The cellular uptake of OI-NPs was detected by confocal microscopy and flow cytometry. 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazoliumbromide assay, flow cytometry, and Hoechst 33342/propidium iodide double staining were used to determine the cytotoxic effect of OI-NP–mediated PSDT on MH7A cells. Fluorescence microscope and fluorescence microplate reader were used to detect reactive oxygen species (ROS) generation.Results: The OI-NPs were a stable and efficient carrier to deliver oxygen and indocyanine green, and enhanced cellular uptake was observed in MH7A cells with the nanoparticles. OI-NP–mediated PSDT caused more serious cell damage and more evident cell apoptosis, compared with other groups. Furthermore, increased generation of intracellular ROS was detected in MH7A cells treated with PSDT. Interestingly, the OI-NP–mediated PSDT-induced cell viability loss was effectively rescued by pretreatment with the ROS scavenger N-acetylcysteine.Conclusion: Multifunctional OI-NPs were successfully developed and characterized for the combined delivery of oxygen and indocyanine green, and OI-NP–mediated PSDT would be a potential cytotoxic treatment for MH7A cells. This study may provide a novel strategy for the treatment of RA and develop a model of theranostic application through phase-transition nanoparticle-mediated PSDT in the future. Keywords: multifunctional nanoparticles, indocyanine green, synoviocytes, reactive oxygen species, apoptosisTang QCui JTian ZSun JWang ZChang SZhu SDove Medical Pressarticlemultifunctional nanoparticlesindocyanine greensynoviocytesreactive oxygen speciesapoptosisMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 12, Pp 381-393 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	multifunctional nanoparticles indocyanine green synoviocytes reactive oxygen species apoptosis Medicine (General) R5-920
spellingShingle	multifunctional nanoparticles indocyanine green synoviocytes reactive oxygen species apoptosis Medicine (General) R5-920 Tang Q Cui J Tian Z Sun J Wang Z Chang S Zhu S Oxygen and indocyanine green loaded phase-transition nanoparticle-mediated photo-sonodynamic cytotoxic effects on rheumatoid arthritis fibroblast-like synoviocytes
description	Qin Tang,1–3 Jianyu Cui,1 Zhonghua Tian,1 Jiangchuan Sun,4 Zhigang Wang,2 Shufang Chang,4 Shenyin Zhu1 1Department of Pharmacy, First Affiliated Hospital of Chongqing Medical University, 2Department of Ultrasound, Institute of Ultrasound Imaging, Second Affiliated Hospital of Chongqing Medical University, 3State Key Laboratory of Ultrasound Engineering in Medicine Co-founded by Chongqing and the Ministry of Science and Technology, 4Department of Obstetrics and Gynecology, Second Affiliated Hospital of Chongqing Medical University, Chongqing, People’s Republic of China Background: Photodynamic therapy and sonodynamic therapy are developing, minimally invasive, and site-specific modalities for cancer therapy. A combined strategy PSDT (photodynamic therapy followed by sonodynamic therapy) has been proposed in this study. Here, we aimed to develop novel biodegradable poly(dl-lactide-co-glycolic acid) phase-transition nanoparticles simultaneously loaded with oxygen and indocyanine green (OI-NPs) and to investigate the cytotoxic effects and the potential mechanisms of OI-NP–mediated PSDT on MH7A synoviocytes.Methods: The OI-NPs were prepared using a modified double emulsion method and the physicochemical properties were determined. The cellular uptake of OI-NPs was detected by confocal microscopy and flow cytometry. 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazoliumbromide assay, flow cytometry, and Hoechst 33342/propidium iodide double staining were used to determine the cytotoxic effect of OI-NP–mediated PSDT on MH7A cells. Fluorescence microscope and fluorescence microplate reader were used to detect reactive oxygen species (ROS) generation.Results: The OI-NPs were a stable and efficient carrier to deliver oxygen and indocyanine green, and enhanced cellular uptake was observed in MH7A cells with the nanoparticles. OI-NP–mediated PSDT caused more serious cell damage and more evident cell apoptosis, compared with other groups. Furthermore, increased generation of intracellular ROS was detected in MH7A cells treated with PSDT. Interestingly, the OI-NP–mediated PSDT-induced cell viability loss was effectively rescued by pretreatment with the ROS scavenger N-acetylcysteine.Conclusion: Multifunctional OI-NPs were successfully developed and characterized for the combined delivery of oxygen and indocyanine green, and OI-NP–mediated PSDT would be a potential cytotoxic treatment for MH7A cells. This study may provide a novel strategy for the treatment of RA and develop a model of theranostic application through phase-transition nanoparticle-mediated PSDT in the future. Keywords: multifunctional nanoparticles, indocyanine green, synoviocytes, reactive oxygen species, apoptosis
format	article
author	Tang Q Cui J Tian Z Sun J Wang Z Chang S Zhu S
author_facet	Tang Q Cui J Tian Z Sun J Wang Z Chang S Zhu S
author_sort	Tang Q
title	Oxygen and indocyanine green loaded phase-transition nanoparticle-mediated photo-sonodynamic cytotoxic effects on rheumatoid arthritis fibroblast-like synoviocytes
title_short	Oxygen and indocyanine green loaded phase-transition nanoparticle-mediated photo-sonodynamic cytotoxic effects on rheumatoid arthritis fibroblast-like synoviocytes
title_full	Oxygen and indocyanine green loaded phase-transition nanoparticle-mediated photo-sonodynamic cytotoxic effects on rheumatoid arthritis fibroblast-like synoviocytes
title_fullStr	Oxygen and indocyanine green loaded phase-transition nanoparticle-mediated photo-sonodynamic cytotoxic effects on rheumatoid arthritis fibroblast-like synoviocytes
title_full_unstemmed	Oxygen and indocyanine green loaded phase-transition nanoparticle-mediated photo-sonodynamic cytotoxic effects on rheumatoid arthritis fibroblast-like synoviocytes
title_sort	oxygen and indocyanine green loaded phase-transition nanoparticle-mediated photo-sonodynamic cytotoxic effects on rheumatoid arthritis fibroblast-like synoviocytes
publisher	Dove Medical Press
publishDate	2017
url	https://doaj.org/article/da6a46a322064e60b29e31001fd364fe
work_keys_str_mv	AT tangq oxygenandindocyaninegreenloadedphasetransitionnanoparticlemediatedphotosonodynamiccytotoxiceffectsonrheumatoidarthritisfibroblastlikesynoviocytes AT cuij oxygenandindocyaninegreenloadedphasetransitionnanoparticlemediatedphotosonodynamiccytotoxiceffectsonrheumatoidarthritisfibroblastlikesynoviocytes AT tianz oxygenandindocyaninegreenloadedphasetransitionnanoparticlemediatedphotosonodynamiccytotoxiceffectsonrheumatoidarthritisfibroblastlikesynoviocytes AT sunj oxygenandindocyaninegreenloadedphasetransitionnanoparticlemediatedphotosonodynamiccytotoxiceffectsonrheumatoidarthritisfibroblastlikesynoviocytes AT wangz oxygenandindocyaninegreenloadedphasetransitionnanoparticlemediatedphotosonodynamiccytotoxiceffectsonrheumatoidarthritisfibroblastlikesynoviocytes AT changs oxygenandindocyaninegreenloadedphasetransitionnanoparticlemediatedphotosonodynamiccytotoxiceffectsonrheumatoidarthritisfibroblastlikesynoviocytes AT zhus oxygenandindocyaninegreenloadedphasetransitionnanoparticlemediatedphotosonodynamiccytotoxiceffectsonrheumatoidarthritisfibroblastlikesynoviocytes
_version_	1718402868285800448

Oxygen and indocyanine green loaded phase-transition nanoparticle-mediated photo-sonodynamic cytotoxic effects on rheumatoid arthritis fibroblast-like synoviocytes

Ejemplares similares