Cold Tolerance during the Reproductive Phase in Chickpea (<i>Cicer arietinum</i> L.) Is Associated with Superior Cold Acclimation Ability Involving Antioxidants and Cryoprotective Solutes in Anthers and Ovules

Chickpea is sensitive to cold stress, especially at reproductive stage, resulting in flower and pod abortion that significantly reduces seed yield. In the present study, we evaluated (a) whether cold acclimation imparts reproductive cold tolerance in chickpea; (b) how genotypes with contrasting sens...

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Autores principales:	Anju Rani, Asha Kiran, Kamal Dev Sharma, P. V. Vara Prasad, Uday C. Jha, Kadambot H. M. Siddique, Harsh Nayyar
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	chilling legumes pollen stigma acclimatization stress Therapeutics. Pharmacology RM1-950
Acceso en línea:	https://doaj.org/article/da7262b870454334bcb5be7928dd3808
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spelling	oai:doaj.org-article:da7262b870454334bcb5be7928dd38082021-11-25T16:26:21ZCold Tolerance during the Reproductive Phase in Chickpea (<i>Cicer arietinum</i> L.) Is Associated with Superior Cold Acclimation Ability Involving Antioxidants and Cryoprotective Solutes in Anthers and Ovules10.3390/antiox101116932076-3921https://doaj.org/article/da7262b870454334bcb5be7928dd38082021-10-01T00:00:00Zhttps://www.mdpi.com/2076-3921/10/11/1693https://doaj.org/toc/2076-3921Chickpea is sensitive to cold stress, especially at reproductive stage, resulting in flower and pod abortion that significantly reduces seed yield. In the present study, we evaluated (a) whether cold acclimation imparts reproductive cold tolerance in chickpea; (b) how genotypes with contrasting sensitivity respond to cold acclimation; and (c) the involvement of cryoprotective solutes and antioxidants in anthers and ovules in cold acclimation. Four chickpea genotypes with contrasting cold sensitivity (cold-tolerant: ICC 17258, ICC 16349; cold-sensitive: ICC 15567, GPF 2) were grown in an outdoor environment for 40 days in November (average maximum/minimum temperature 24.9/15.9 °C) before being subjected to cold stress (13/7 °C), with or without cold acclimation in a controlled environment of walk-in-growth chambers. The 42-d cold acclimation involved 7 d exposure at each temperature beginning with 23/15 °C, 21/13 °C, 20/12 °C, 20/10 °C, 18/8 °C, 15/8 °C (12 h/12 h day/night), prior to exposing the plants to cold stress (13/7 °C, 12 h/12 h day/night; 700 μmol m<sup>−2</sup> s<sup>−1</sup> light intensity; 65–70% relative humidity). Cold acclimation remarkably reduced low temperature-induced leaf damage (as membrane integrity, leaf water status, stomatal conductance, photosynthetic pigments, and chlorophyll fluorescence) under cold stress in all four genotypes. It only reduced anther and ovule damage in cold-tolerant genotypes due to improved antioxidative ability, measured as enzymatic (superoxide dismutase, catalase, ascorbate peroxidase, and glutathione reductase) and non-enzymatic (ascorbate and reduced glutathione), solutes (particularly sucrose and γ-aminobutyric acid) leading to improving reproductive function and yield traits, whereas cold-sensitive genotypes were not responsive. The study concluded that cold tolerance in chickpea appears to be related to the better ability of anthers and ovules to acclimate, involving various antioxidants and cryoprotective solutes. This information will be useful in directing efforts toward increasing cold tolerance in chickpea.Anju RaniAsha KiranKamal Dev SharmaP. V. Vara PrasadUday C. JhaKadambot H. M. SiddiqueHarsh NayyarMDPI AGarticlechillinglegumespollenstigmaacclimatizationstressTherapeutics. PharmacologyRM1-950ENAntioxidants, Vol 10, Iss 1693, p 1693 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	chilling legumes pollen stigma acclimatization stress Therapeutics. Pharmacology RM1-950
spellingShingle	chilling legumes pollen stigma acclimatization stress Therapeutics. Pharmacology RM1-950 Anju Rani Asha Kiran Kamal Dev Sharma P. V. Vara Prasad Uday C. Jha Kadambot H. M. Siddique Harsh Nayyar Cold Tolerance during the Reproductive Phase in Chickpea (<i>Cicer arietinum</i> L.) Is Associated with Superior Cold Acclimation Ability Involving Antioxidants and Cryoprotective Solutes in Anthers and Ovules
description	Chickpea is sensitive to cold stress, especially at reproductive stage, resulting in flower and pod abortion that significantly reduces seed yield. In the present study, we evaluated (a) whether cold acclimation imparts reproductive cold tolerance in chickpea; (b) how genotypes with contrasting sensitivity respond to cold acclimation; and (c) the involvement of cryoprotective solutes and antioxidants in anthers and ovules in cold acclimation. Four chickpea genotypes with contrasting cold sensitivity (cold-tolerant: ICC 17258, ICC 16349; cold-sensitive: ICC 15567, GPF 2) were grown in an outdoor environment for 40 days in November (average maximum/minimum temperature 24.9/15.9 °C) before being subjected to cold stress (13/7 °C), with or without cold acclimation in a controlled environment of walk-in-growth chambers. The 42-d cold acclimation involved 7 d exposure at each temperature beginning with 23/15 °C, 21/13 °C, 20/12 °C, 20/10 °C, 18/8 °C, 15/8 °C (12 h/12 h day/night), prior to exposing the plants to cold stress (13/7 °C, 12 h/12 h day/night; 700 μmol m<sup>−2</sup> s<sup>−1</sup> light intensity; 65–70% relative humidity). Cold acclimation remarkably reduced low temperature-induced leaf damage (as membrane integrity, leaf water status, stomatal conductance, photosynthetic pigments, and chlorophyll fluorescence) under cold stress in all four genotypes. It only reduced anther and ovule damage in cold-tolerant genotypes due to improved antioxidative ability, measured as enzymatic (superoxide dismutase, catalase, ascorbate peroxidase, and glutathione reductase) and non-enzymatic (ascorbate and reduced glutathione), solutes (particularly sucrose and γ-aminobutyric acid) leading to improving reproductive function and yield traits, whereas cold-sensitive genotypes were not responsive. The study concluded that cold tolerance in chickpea appears to be related to the better ability of anthers and ovules to acclimate, involving various antioxidants and cryoprotective solutes. This information will be useful in directing efforts toward increasing cold tolerance in chickpea.
format	article
author	Anju Rani Asha Kiran Kamal Dev Sharma P. V. Vara Prasad Uday C. Jha Kadambot H. M. Siddique Harsh Nayyar
author_facet	Anju Rani Asha Kiran Kamal Dev Sharma P. V. Vara Prasad Uday C. Jha Kadambot H. M. Siddique Harsh Nayyar
author_sort	Anju Rani
title	Cold Tolerance during the Reproductive Phase in Chickpea (<i>Cicer arietinum</i> L.) Is Associated with Superior Cold Acclimation Ability Involving Antioxidants and Cryoprotective Solutes in Anthers and Ovules
title_short	Cold Tolerance during the Reproductive Phase in Chickpea (<i>Cicer arietinum</i> L.) Is Associated with Superior Cold Acclimation Ability Involving Antioxidants and Cryoprotective Solutes in Anthers and Ovules
title_full	Cold Tolerance during the Reproductive Phase in Chickpea (<i>Cicer arietinum</i> L.) Is Associated with Superior Cold Acclimation Ability Involving Antioxidants and Cryoprotective Solutes in Anthers and Ovules
title_fullStr	Cold Tolerance during the Reproductive Phase in Chickpea (<i>Cicer arietinum</i> L.) Is Associated with Superior Cold Acclimation Ability Involving Antioxidants and Cryoprotective Solutes in Anthers and Ovules
title_full_unstemmed	Cold Tolerance during the Reproductive Phase in Chickpea (<i>Cicer arietinum</i> L.) Is Associated with Superior Cold Acclimation Ability Involving Antioxidants and Cryoprotective Solutes in Anthers and Ovules
title_sort	cold tolerance during the reproductive phase in chickpea (<i>cicer arietinum</i> l.) is associated with superior cold acclimation ability involving antioxidants and cryoprotective solutes in anthers and ovules
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/da7262b870454334bcb5be7928dd3808
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Cold Tolerance during the Reproductive Phase in Chickpea (<i>Cicer arietinum</i> L.) Is Associated with Superior Cold Acclimation Ability Involving Antioxidants and Cryoprotective Solutes in Anthers and Ovules

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