Cellular trafficking and anticancer activity of Garcinia mangostana extract-encapsulated polymeric nanoparticles

Porntip Pan-In,1,2 Supason Wanichwecharungruang,3,4 Justin Hanes,2,5 Anthony J Kim2,6,7 1Program in Biotechnology, Faculty of Science, Chulalongkorn University, Bangkok, Thailand; 2Center for Nanomedicine, Johns Hopkins University School of Medicine, Baltimore, MD, USA; 3Department of Chemistry, F...

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Autores principales:	Pan-In P, Wanichwecharungruang S, Hanes J, Kim AJ
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2014
Materias:	Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/da8f1e5335b84721922594bba444eb13
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spelling	oai:doaj.org-article:da8f1e5335b84721922594bba444eb132021-12-02T01:50:38ZCellular trafficking and anticancer activity of Garcinia mangostana extract-encapsulated polymeric nanoparticles1178-2013https://doaj.org/article/da8f1e5335b84721922594bba444eb132014-08-01T00:00:00Zhttp://www.dovepress.com/cellular-trafficking-and-anticancer-activity-of-garcinia-mangostana-ex-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013 Porntip Pan-In,1,2 Supason Wanichwecharungruang,3,4 Justin Hanes,2,5 Anthony J Kim2,6,7 1Program in Biotechnology, Faculty of Science, Chulalongkorn University, Bangkok, Thailand; 2Center for Nanomedicine, Johns Hopkins University School of Medicine, Baltimore, MD, USA; 3Department of Chemistry, Faculty of Science, Chulalongkorn University, Bangkok, Thailand; 4Nanotec-CU Center of Excellence on Food and Agriculture, Bangkok, Thailand; 5Department of Ophthalmology, Biomedical Engineering, Chemical and Biomolecular Engineering, Neurosurgery, and Oncology, Johns Hopkins University School of Medicine, 6Department of Neurosurgery, University of Maryland School of Medicine, 7Department of Pharmaceutical Sciences, University of Maryland School of Pharmacy, Baltimore, MD, USA Abstract: Garcinia mangostana Linn extract (GME) is a natural product that has received considerable attention in cancer therapy, and has the potential to reduce side effects of chemotherapeutics and improve efficacy. We formulated GME-encapsulated ethyl cellulose (GME-EC) and a polymer blend of ethyl cellulose and methyl cellulose (GME-EC/MC) nanoparticles. We achieved high drug-loading and encapsulation efficiency using a solvent-displacement method with particle sizes around 250 nm. Cellular uptake and accumulation of GME was higher for GME-encapsulated nanoparticles compared to free GME. In vitro cytotoxicity analysis showed effective anticancer activity of GME-EC and GME-EC/MC nanoparticles in HeLa cells in a dose-dependent manner. GME-EC/MC nanoparticles showed approximately twofold-higher anticancer activity compared to GME-EC nanoparticles, likely due to their enhanced bioavailability. GME-encapsulated nanoparticles primarily entered HeLa cells by clathrin-mediated endocytosis and trafficked through the endolysosomal pathway. As far as we know, this is the first report on the cellular uptake and intracellular trafficking mechanism of drug-loaded cellulose-based nanoparticles. In summary, encapsulation of GME using cellulose-derivative nanoparticles – GME-EC and GME-EC/MC nanoparticles – successfully improved the bioavailability of GME in aqueous solution, enhanced cellular uptake, and displayed effective anticancer activity. Keywords: phytotherapy, cancer, nanoparticles, cellulose, intracellular traffickingPan-In PWanichwecharungruang SHanes JKim AJDove Medical PressarticleMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2014, Iss Issue 1, Pp 3677-3686 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine (General) R5-920
spellingShingle	Medicine (General) R5-920 Pan-In P Wanichwecharungruang S Hanes J Kim AJ Cellular trafficking and anticancer activity of Garcinia mangostana extract-encapsulated polymeric nanoparticles
description	Porntip Pan-In,1,2 Supason Wanichwecharungruang,3,4 Justin Hanes,2,5 Anthony J Kim2,6,7 1Program in Biotechnology, Faculty of Science, Chulalongkorn University, Bangkok, Thailand; 2Center for Nanomedicine, Johns Hopkins University School of Medicine, Baltimore, MD, USA; 3Department of Chemistry, Faculty of Science, Chulalongkorn University, Bangkok, Thailand; 4Nanotec-CU Center of Excellence on Food and Agriculture, Bangkok, Thailand; 5Department of Ophthalmology, Biomedical Engineering, Chemical and Biomolecular Engineering, Neurosurgery, and Oncology, Johns Hopkins University School of Medicine, 6Department of Neurosurgery, University of Maryland School of Medicine, 7Department of Pharmaceutical Sciences, University of Maryland School of Pharmacy, Baltimore, MD, USA Abstract: Garcinia mangostana Linn extract (GME) is a natural product that has received considerable attention in cancer therapy, and has the potential to reduce side effects of chemotherapeutics and improve efficacy. We formulated GME-encapsulated ethyl cellulose (GME-EC) and a polymer blend of ethyl cellulose and methyl cellulose (GME-EC/MC) nanoparticles. We achieved high drug-loading and encapsulation efficiency using a solvent-displacement method with particle sizes around 250 nm. Cellular uptake and accumulation of GME was higher for GME-encapsulated nanoparticles compared to free GME. In vitro cytotoxicity analysis showed effective anticancer activity of GME-EC and GME-EC/MC nanoparticles in HeLa cells in a dose-dependent manner. GME-EC/MC nanoparticles showed approximately twofold-higher anticancer activity compared to GME-EC nanoparticles, likely due to their enhanced bioavailability. GME-encapsulated nanoparticles primarily entered HeLa cells by clathrin-mediated endocytosis and trafficked through the endolysosomal pathway. As far as we know, this is the first report on the cellular uptake and intracellular trafficking mechanism of drug-loaded cellulose-based nanoparticles. In summary, encapsulation of GME using cellulose-derivative nanoparticles – GME-EC and GME-EC/MC nanoparticles – successfully improved the bioavailability of GME in aqueous solution, enhanced cellular uptake, and displayed effective anticancer activity. Keywords: phytotherapy, cancer, nanoparticles, cellulose, intracellular trafficking
format	article
author	Pan-In P Wanichwecharungruang S Hanes J Kim AJ
author_facet	Pan-In P Wanichwecharungruang S Hanes J Kim AJ
author_sort	Pan-In P
title	Cellular trafficking and anticancer activity of Garcinia mangostana extract-encapsulated polymeric nanoparticles
title_short	Cellular trafficking and anticancer activity of Garcinia mangostana extract-encapsulated polymeric nanoparticles
title_full	Cellular trafficking and anticancer activity of Garcinia mangostana extract-encapsulated polymeric nanoparticles
title_fullStr	Cellular trafficking and anticancer activity of Garcinia mangostana extract-encapsulated polymeric nanoparticles
title_full_unstemmed	Cellular trafficking and anticancer activity of Garcinia mangostana extract-encapsulated polymeric nanoparticles
title_sort	cellular trafficking and anticancer activity of garcinia mangostana extract-encapsulated polymeric nanoparticles
publisher	Dove Medical Press
publishDate	2014
url	https://doaj.org/article/da8f1e5335b84721922594bba444eb13
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Cellular trafficking and anticancer activity of Garcinia mangostana extract-encapsulated polymeric nanoparticles

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