Development of a Molecular Serotyping Scheme for Morganella morganii

Morganella morganii, which is often regarded as a human commensal organism, can be an opportunistic pathogen, causing a variety of clinical infections with serious morbidity and mortality. An efficient and convenient method for subtyping and identifying M. morganii strains in epidemiological surveil...

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Autores principales:	Bin Liu, Xi Guo, Jing Wang, Pan Wu, Shujie Li, Lu Feng, Lei Wang
Formato:	article
Lenguaje:	EN
Publicado:	Frontiers Media S.A. 2021
Materias:	Morganella morganii surface polysaccharide O-antigen gene cluster serotyping microsphere-based suspension array Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/da90c6747e09480d905e2c08dfa6690b
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spelling	oai:doaj.org-article:da90c6747e09480d905e2c08dfa6690b2021-11-30T11:55:38ZDevelopment of a Molecular Serotyping Scheme for Morganella morganii1664-302X10.3389/fmicb.2021.791165https://doaj.org/article/da90c6747e09480d905e2c08dfa6690b2021-11-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fmicb.2021.791165/fullhttps://doaj.org/toc/1664-302XMorganella morganii, which is often regarded as a human commensal organism, can be an opportunistic pathogen, causing a variety of clinical infections with serious morbidity and mortality. An efficient and convenient method for subtyping and identifying M. morganii strains in epidemiological surveillance and control is urgently needed. Serotyping based on bacterial surface polysaccharide antigens (O-antigen or K-antigens) is a standard subtyping method for many gram-negative bacteria. Here, through whole genome sequencing and comparative genomics analysis of 27 strains, we developed a molecular serotyping scheme based on the genetic variation of O-antigen gene clusters (O-AGC) in M. morganii, and 11 distinct O-AGC types were identified. A conventional serotyping scheme was also developed by the production of antisera and agglutination experiments, which was shown to be perfectly consistent with the molecular serotyping scheme, confirming that the variation in M. morganii O-AGC correlated with phenotypic O-antigen diversification. Furthermore, a microsphere-based suspension array (MSA) with high specificity was developed based on the specific genes within each O-AGC type. The sensitivity of MSA was determined to be 0.1 ng of genomic DNA and 103 CFU of pure culture. We further analyzed 104 M. morganii genomes available in GenBank, and an additional six novel O-AGC types were identified, indicating that the extension of this molecular serotyping scheme is convenient. Our work provides an important tool for the detection and epidemiological surveillance of M. morganii, and this method has the potential to be widely utilized, especially for bacterial genera/species without an efficient typing approach.Bin LiuBin LiuXi GuoXi GuoJing WangPan WuShujie LiLu FengLu FengBin LiuBin LiuLei WangLei WangFrontiers Media S.A.articleMorganella morganiisurface polysaccharideO-antigen gene clusterserotypingmicrosphere-based suspension arrayMicrobiologyQR1-502ENFrontiers in Microbiology, Vol 12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Morganella morganii surface polysaccharide O-antigen gene cluster serotyping microsphere-based suspension array Microbiology QR1-502
spellingShingle	Morganella morganii surface polysaccharide O-antigen gene cluster serotyping microsphere-based suspension array Microbiology QR1-502 Bin Liu Bin Liu Xi Guo Xi Guo Jing Wang Pan Wu Shujie Li Lu Feng Lu Feng Bin Liu Bin Liu Lei Wang Lei Wang Development of a Molecular Serotyping Scheme for Morganella morganii
description	Morganella morganii, which is often regarded as a human commensal organism, can be an opportunistic pathogen, causing a variety of clinical infections with serious morbidity and mortality. An efficient and convenient method for subtyping and identifying M. morganii strains in epidemiological surveillance and control is urgently needed. Serotyping based on bacterial surface polysaccharide antigens (O-antigen or K-antigens) is a standard subtyping method for many gram-negative bacteria. Here, through whole genome sequencing and comparative genomics analysis of 27 strains, we developed a molecular serotyping scheme based on the genetic variation of O-antigen gene clusters (O-AGC) in M. morganii, and 11 distinct O-AGC types were identified. A conventional serotyping scheme was also developed by the production of antisera and agglutination experiments, which was shown to be perfectly consistent with the molecular serotyping scheme, confirming that the variation in M. morganii O-AGC correlated with phenotypic O-antigen diversification. Furthermore, a microsphere-based suspension array (MSA) with high specificity was developed based on the specific genes within each O-AGC type. The sensitivity of MSA was determined to be 0.1 ng of genomic DNA and 103 CFU of pure culture. We further analyzed 104 M. morganii genomes available in GenBank, and an additional six novel O-AGC types were identified, indicating that the extension of this molecular serotyping scheme is convenient. Our work provides an important tool for the detection and epidemiological surveillance of M. morganii, and this method has the potential to be widely utilized, especially for bacterial genera/species without an efficient typing approach.
format	article
author	Bin Liu Bin Liu Xi Guo Xi Guo Jing Wang Pan Wu Shujie Li Lu Feng Lu Feng Bin Liu Bin Liu Lei Wang Lei Wang
author_facet	Bin Liu Bin Liu Xi Guo Xi Guo Jing Wang Pan Wu Shujie Li Lu Feng Lu Feng Bin Liu Bin Liu Lei Wang Lei Wang
author_sort	Bin Liu
title	Development of a Molecular Serotyping Scheme for Morganella morganii
title_short	Development of a Molecular Serotyping Scheme for Morganella morganii
title_full	Development of a Molecular Serotyping Scheme for Morganella morganii
title_fullStr	Development of a Molecular Serotyping Scheme for Morganella morganii
title_full_unstemmed	Development of a Molecular Serotyping Scheme for Morganella morganii
title_sort	development of a molecular serotyping scheme for morganella morganii
publisher	Frontiers Media S.A.
publishDate	2021
url	https://doaj.org/article/da90c6747e09480d905e2c08dfa6690b
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Development of a Molecular Serotyping Scheme for Morganella morganii

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