Genome-scale measurement of off-target activity using Cas9 toxicity in high-throughput screens

CRISPR-Cas9 screens are powerful high-throughput tools but can be confounded by nuclease toxicity. Here the authors design a library of variable length gRNAs with thousands of negative controls, including the targeting of ‘safe’ loci to account for on-target site DNA damage toxicity.

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Autores principales:	David W. Morgens, Michael Wainberg, Evan A. Boyle, Oana Ursu, Carlos L. Araya, C. Kimberly Tsui, Michael S. Haney, Gaelen T. Hess, Kyuho Han, Edwin E. Jeng, Amy Li, Michael P. Snyder, William J. Greenleaf, Anshul Kundaje, Michael C. Bassik
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Science Q
Acceso en línea:	https://doaj.org/article/da97c345b50241ee96636d0cb1d3e3fc
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spelling	oai:doaj.org-article:da97c345b50241ee96636d0cb1d3e3fc2021-12-02T17:01:23ZGenome-scale measurement of off-target activity using Cas9 toxicity in high-throughput screens10.1038/ncomms151782041-1723https://doaj.org/article/da97c345b50241ee96636d0cb1d3e3fc2017-05-01T00:00:00Zhttps://doi.org/10.1038/ncomms15178https://doaj.org/toc/2041-1723CRISPR-Cas9 screens are powerful high-throughput tools but can be confounded by nuclease toxicity. Here the authors design a library of variable length gRNAs with thousands of negative controls, including the targeting of ‘safe’ loci to account for on-target site DNA damage toxicity.David W. MorgensMichael WainbergEvan A. BoyleOana UrsuCarlos L. ArayaC. Kimberly TsuiMichael S. HaneyGaelen T. HessKyuho HanEdwin E. JengAmy LiMichael P. SnyderWilliam J. GreenleafAnshul KundajeMichael C. BassikNature PortfolioarticleScienceQENNature Communications, Vol 8, Iss 1, Pp 1-8 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Science Q
spellingShingle	Science Q David W. Morgens Michael Wainberg Evan A. Boyle Oana Ursu Carlos L. Araya C. Kimberly Tsui Michael S. Haney Gaelen T. Hess Kyuho Han Edwin E. Jeng Amy Li Michael P. Snyder William J. Greenleaf Anshul Kundaje Michael C. Bassik Genome-scale measurement of off-target activity using Cas9 toxicity in high-throughput screens
description	CRISPR-Cas9 screens are powerful high-throughput tools but can be confounded by nuclease toxicity. Here the authors design a library of variable length gRNAs with thousands of negative controls, including the targeting of ‘safe’ loci to account for on-target site DNA damage toxicity.
format	article
author	David W. Morgens Michael Wainberg Evan A. Boyle Oana Ursu Carlos L. Araya C. Kimberly Tsui Michael S. Haney Gaelen T. Hess Kyuho Han Edwin E. Jeng Amy Li Michael P. Snyder William J. Greenleaf Anshul Kundaje Michael C. Bassik
author_facet	David W. Morgens Michael Wainberg Evan A. Boyle Oana Ursu Carlos L. Araya C. Kimberly Tsui Michael S. Haney Gaelen T. Hess Kyuho Han Edwin E. Jeng Amy Li Michael P. Snyder William J. Greenleaf Anshul Kundaje Michael C. Bassik
author_sort	David W. Morgens
title	Genome-scale measurement of off-target activity using Cas9 toxicity in high-throughput screens
title_short	Genome-scale measurement of off-target activity using Cas9 toxicity in high-throughput screens
title_full	Genome-scale measurement of off-target activity using Cas9 toxicity in high-throughput screens
title_fullStr	Genome-scale measurement of off-target activity using Cas9 toxicity in high-throughput screens
title_full_unstemmed	Genome-scale measurement of off-target activity using Cas9 toxicity in high-throughput screens
title_sort	genome-scale measurement of off-target activity using cas9 toxicity in high-throughput screens
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/da97c345b50241ee96636d0cb1d3e3fc
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Genome-scale measurement of off-target activity using Cas9 toxicity in high-throughput screens

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