In vitro and in vivo protein release and anti-ischemia/reperfusion injury properties of bone morphogenetic protein-2-loaded glycyrrhetinic acid-poly(ethylene glycol)-b-poly(L-lysine) nanoparticles

Fang Shan,1 YuJuan Liu,1 Haiying Jiang,2 Fei Tong2 1Department of Physiology, Hexi University Medical College, Zhangye, 2Department of Pathology and Pathophysiology, Provincial Key Discipline of Pharmacology, Jiaxing University Medical College, Jiaxing, People’s Republic of China Abstrac...

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Autores principales:	Shan F, Liu Y, Jiang H, Tong F
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2017
Materias:	GA-PEG-b-PLL PIC micelles BMP-2 HI/RI Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/da9cd42eb1c1493c8300bec5b1804f91
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spelling	oai:doaj.org-article:da9cd42eb1c1493c8300bec5b1804f912021-12-02T07:48:10ZIn vitro and in vivo protein release and anti-ischemia/reperfusion injury properties of bone morphogenetic protein-2-loaded glycyrrhetinic acid-poly(ethylene glycol)-b-poly(L-lysine) nanoparticles1178-2013https://doaj.org/article/da9cd42eb1c1493c8300bec5b1804f912017-10-01T00:00:00Zhttps://www.dovepress.com/in-vitro-and-in-vivo-protein-release-and-anti-ischemiareperfusion-inju-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Fang Shan,1 YuJuan Liu,1 Haiying Jiang,2 Fei Tong2 1Department of Physiology, Hexi University Medical College, Zhangye, 2Department of Pathology and Pathophysiology, Provincial Key Discipline of Pharmacology, Jiaxing University Medical College, Jiaxing, People’s Republic of China Abstract: Here, we describe a bone morphogenetic protein-2 (BMP-2) nanocarrier based on glycyrrhetinic acid (GA)-poly(ethylene glycol) (PEG)-b-poly(L-lysine) (PLL). A protein nanocarrier was synthesized, characterized and evaluated as a BMP-2 delivery system. The designed nanocarrier was synthesized based on the ring-opening polymerization of amino acid N-carboxyanhydride. The final product was measured with 1H nuclear magnetic resonance. GA-PEG-b-PLL nanocarrier could combine with BMP-2 through electrostatic interaction to form polyion complex (PIC) micelles. BMP-2 could be rapidly and efficiently encapsulated through the GA-PEG-b-PLL nanocarrier under physiological conditions, exhibiting efficient encapsulation and sustained release. In addition, the GA-PEG-b-PLL-mediated BMP-2 delivery system could target the liver against hepatic diseases as it has GA-binding receptors. The anti-hepatic ischemia/reperfusion injury (anti-HI/RI) effect of BMP-2/GA-PEG-b-PLL PIC micelles was investigated in rats using free BMP-2 and BMP-2/PEG-b-PLL PIC micelles as controls, and the results showed that BMP-2/GA-PEG-b-PLL PIC micelles indicated significantly enhanced anti-HI/RI property compared to BMP-2 and BMP-2/PEG-b-PLL. All results suggested that GA-PEG-b-PLL could be used as a potential BMP-2 nanocarrier. Keywords: GA-PEG-b-PLL, PIC micelles, BMP-2, HI/RI Shan FLiu YJiang HTong FDove Medical PressarticleGA-PEG-b-PLLPIC micellesBMP-2HI/RIMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 12, Pp 7613-7625 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	GA-PEG-b-PLL PIC micelles BMP-2 HI/RI Medicine (General) R5-920
spellingShingle	GA-PEG-b-PLL PIC micelles BMP-2 HI/RI Medicine (General) R5-920 Shan F Liu Y Jiang H Tong F In vitro and in vivo protein release and anti-ischemia/reperfusion injury properties of bone morphogenetic protein-2-loaded glycyrrhetinic acid-poly(ethylene glycol)-b-poly(L-lysine) nanoparticles
description	Fang Shan,1 YuJuan Liu,1 Haiying Jiang,2 Fei Tong2 1Department of Physiology, Hexi University Medical College, Zhangye, 2Department of Pathology and Pathophysiology, Provincial Key Discipline of Pharmacology, Jiaxing University Medical College, Jiaxing, People’s Republic of China Abstract: Here, we describe a bone morphogenetic protein-2 (BMP-2) nanocarrier based on glycyrrhetinic acid (GA)-poly(ethylene glycol) (PEG)-b-poly(L-lysine) (PLL). A protein nanocarrier was synthesized, characterized and evaluated as a BMP-2 delivery system. The designed nanocarrier was synthesized based on the ring-opening polymerization of amino acid N-carboxyanhydride. The final product was measured with 1H nuclear magnetic resonance. GA-PEG-b-PLL nanocarrier could combine with BMP-2 through electrostatic interaction to form polyion complex (PIC) micelles. BMP-2 could be rapidly and efficiently encapsulated through the GA-PEG-b-PLL nanocarrier under physiological conditions, exhibiting efficient encapsulation and sustained release. In addition, the GA-PEG-b-PLL-mediated BMP-2 delivery system could target the liver against hepatic diseases as it has GA-binding receptors. The anti-hepatic ischemia/reperfusion injury (anti-HI/RI) effect of BMP-2/GA-PEG-b-PLL PIC micelles was investigated in rats using free BMP-2 and BMP-2/PEG-b-PLL PIC micelles as controls, and the results showed that BMP-2/GA-PEG-b-PLL PIC micelles indicated significantly enhanced anti-HI/RI property compared to BMP-2 and BMP-2/PEG-b-PLL. All results suggested that GA-PEG-b-PLL could be used as a potential BMP-2 nanocarrier. Keywords: GA-PEG-b-PLL, PIC micelles, BMP-2, HI/RI
format	article
author	Shan F Liu Y Jiang H Tong F
author_facet	Shan F Liu Y Jiang H Tong F
author_sort	Shan F
title	In vitro and in vivo protein release and anti-ischemia/reperfusion injury properties of bone morphogenetic protein-2-loaded glycyrrhetinic acid-poly(ethylene glycol)-b-poly(L-lysine) nanoparticles
title_short	In vitro and in vivo protein release and anti-ischemia/reperfusion injury properties of bone morphogenetic protein-2-loaded glycyrrhetinic acid-poly(ethylene glycol)-b-poly(L-lysine) nanoparticles
title_full	In vitro and in vivo protein release and anti-ischemia/reperfusion injury properties of bone morphogenetic protein-2-loaded glycyrrhetinic acid-poly(ethylene glycol)-b-poly(L-lysine) nanoparticles
title_fullStr	In vitro and in vivo protein release and anti-ischemia/reperfusion injury properties of bone morphogenetic protein-2-loaded glycyrrhetinic acid-poly(ethylene glycol)-b-poly(L-lysine) nanoparticles
title_full_unstemmed	In vitro and in vivo protein release and anti-ischemia/reperfusion injury properties of bone morphogenetic protein-2-loaded glycyrrhetinic acid-poly(ethylene glycol)-b-poly(L-lysine) nanoparticles
title_sort	in vitro and in vivo protein release and anti-ischemia/reperfusion injury properties of bone morphogenetic protein-2-loaded glycyrrhetinic acid-poly(ethylene glycol)-b-poly(l-lysine) nanoparticles
publisher	Dove Medical Press
publishDate	2017
url	https://doaj.org/article/da9cd42eb1c1493c8300bec5b1804f91
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In vitro and in vivo protein release and anti-ischemia/reperfusion injury properties of bone morphogenetic protein-2-loaded glycyrrhetinic acid-poly(ethylene glycol)-b-poly(L-lysine) nanoparticles

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