Color-tunable bioluminescence imaging portfolio for cell imaging

Abstract The present study describes a color-tunable imaging portfolio together with twelve novel coelenterazine (CTZ) analogues. The three groups of CTZ analogues create diverse hues of bioluminescence (BL) ranging from blue to far red with marine luciferases. We found that the hue completes the wh...

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Autores principales:	Shota Tamaki, Nobuo Kitada, Masahiro Kiyama, Rika Fujii, Takashi Hirano, Sung Bae Kim, Shojiro Maki
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/da9cdbbd33cb49d79b46b76fbfc03811
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spelling	oai:doaj.org-article:da9cdbbd33cb49d79b46b76fbfc038112021-12-02T13:27:32ZColor-tunable bioluminescence imaging portfolio for cell imaging10.1038/s41598-021-81430-12045-2322https://doaj.org/article/da9cdbbd33cb49d79b46b76fbfc038112021-01-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-81430-1https://doaj.org/toc/2045-2322Abstract The present study describes a color-tunable imaging portfolio together with twelve novel coelenterazine (CTZ) analogues. The three groups of CTZ analogues create diverse hues of bioluminescence (BL) ranging from blue to far red with marine luciferases. We found that the hue completes the whole color palette in the visible region and shows red-shifted BL with a marine luciferase: for example, Renilla luciferase 8 (RLuc8) and Artificial Luciferase 16 (ALuc16) show 187 nm- and 105 nm-redshifted spectra, respectively, by simply replacing the substrate CTZ with 1d. The optical properties of the new CTZ analogues were investigated such as the kinetic parameters, dose dependency, and luciferase specificity. The 2-series CTZ analogues interestingly have specificity to ALucs and are completely dark with RLuc derivatives, and 3d is highly specific to only NanoLuc. We further determined the theoretical background of the red-shifted BL maximum wavelengths (λ BL) values according to the extended π conjugation of the CTZ backbone using Density Functional Theory (DFT) calculations. This color-tunable BL imaging system provides a useful multicolor imaging portfolio that efficiently images molecular events in mammalian cells.Shota TamakiNobuo KitadaMasahiro KiyamaRika FujiiTakashi HiranoSung Bae KimShojiro MakiNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-10 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Shota Tamaki Nobuo Kitada Masahiro Kiyama Rika Fujii Takashi Hirano Sung Bae Kim Shojiro Maki Color-tunable bioluminescence imaging portfolio for cell imaging
description	Abstract The present study describes a color-tunable imaging portfolio together with twelve novel coelenterazine (CTZ) analogues. The three groups of CTZ analogues create diverse hues of bioluminescence (BL) ranging from blue to far red with marine luciferases. We found that the hue completes the whole color palette in the visible region and shows red-shifted BL with a marine luciferase: for example, Renilla luciferase 8 (RLuc8) and Artificial Luciferase 16 (ALuc16) show 187 nm- and 105 nm-redshifted spectra, respectively, by simply replacing the substrate CTZ with 1d. The optical properties of the new CTZ analogues were investigated such as the kinetic parameters, dose dependency, and luciferase specificity. The 2-series CTZ analogues interestingly have specificity to ALucs and are completely dark with RLuc derivatives, and 3d is highly specific to only NanoLuc. We further determined the theoretical background of the red-shifted BL maximum wavelengths (λ BL) values according to the extended π conjugation of the CTZ backbone using Density Functional Theory (DFT) calculations. This color-tunable BL imaging system provides a useful multicolor imaging portfolio that efficiently images molecular events in mammalian cells.
format	article
author	Shota Tamaki Nobuo Kitada Masahiro Kiyama Rika Fujii Takashi Hirano Sung Bae Kim Shojiro Maki
author_facet	Shota Tamaki Nobuo Kitada Masahiro Kiyama Rika Fujii Takashi Hirano Sung Bae Kim Shojiro Maki
author_sort	Shota Tamaki
title	Color-tunable bioluminescence imaging portfolio for cell imaging
title_short	Color-tunable bioluminescence imaging portfolio for cell imaging
title_full	Color-tunable bioluminescence imaging portfolio for cell imaging
title_fullStr	Color-tunable bioluminescence imaging portfolio for cell imaging
title_full_unstemmed	Color-tunable bioluminescence imaging portfolio for cell imaging
title_sort	color-tunable bioluminescence imaging portfolio for cell imaging
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/da9cdbbd33cb49d79b46b76fbfc03811
work_keys_str_mv	AT shotatamaki colortunablebioluminescenceimagingportfolioforcellimaging AT nobuokitada colortunablebioluminescenceimagingportfolioforcellimaging AT masahirokiyama colortunablebioluminescenceimagingportfolioforcellimaging AT rikafujii colortunablebioluminescenceimagingportfolioforcellimaging AT takashihirano colortunablebioluminescenceimagingportfolioforcellimaging AT sungbaekim colortunablebioluminescenceimagingportfolioforcellimaging AT shojiromaki colortunablebioluminescenceimagingportfolioforcellimaging
_version_	1718393004794839040

Color-tunable bioluminescence imaging portfolio for cell imaging

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