Harvesting electricity with Geobacter bremensis isolated from compost.

Electrochemically active (EA) biofilms were formed on metallic dimensionally stable anode-type electrode (DSA), embedded in garden compost and polarized at +0.50 V/SCE. Analysis of 16S rRNA gene libraries revealed that biofilms were heavily enriched in Deltaproteobacteria in comparison to control bi...

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Autores principales:	Olivier Nercessian, Sandrine Parot, Marie-Line Délia, Alain Bergel, Wafa Achouak
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2012
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/daa0fb4628ef412bb557cdeb52cf7cb3
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spelling	oai:doaj.org-article:daa0fb4628ef412bb557cdeb52cf7cb32021-11-18T07:24:00ZHarvesting electricity with Geobacter bremensis isolated from compost.1932-620310.1371/journal.pone.0034216https://doaj.org/article/daa0fb4628ef412bb557cdeb52cf7cb32012-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/22470538/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203Electrochemically active (EA) biofilms were formed on metallic dimensionally stable anode-type electrode (DSA), embedded in garden compost and polarized at +0.50 V/SCE. Analysis of 16S rRNA gene libraries revealed that biofilms were heavily enriched in Deltaproteobacteria in comparison to control biofilms formed on non-polarized electrodes, which were preferentially composed of Gammaproteobacteria and Firmicutes. Among Deltaproteobacteria, sequences affiliated with Pelobacter and Geobacter genera were identified. A bacterial consortium was cultivated, in which 25 isolates were identified as Geobacter bremensis. Pure cultures of 4 different G. bremensis isolates gave higher current densities (1400 mA/m(2) on DSA, 2490 mA/m(2) on graphite) than the original multi-species biofilms (in average 300 mA/m(2) on DSA) and the G. bremensis DSM type strain (100-300 A/m(2) on DSA; 2485 mA/m(2) on graphite). FISH analysis confirmed that G. bremensis represented a minor fraction in the original EA biofilm, in which species related to Pelobacter genus were predominant. The Pelobacter type strain did not show EA capacity, which can explain the lower performance of the multi-species biofilms. These results stressed the great interest of extracting and culturing pure EA strains from wild EA biofilms to improve the current density provided by microbial anodes.Olivier NercessianSandrine ParotMarie-Line DéliaAlain BergelWafa AchouakPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 7, Iss 3, p e34216 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Olivier Nercessian Sandrine Parot Marie-Line Délia Alain Bergel Wafa Achouak Harvesting electricity with Geobacter bremensis isolated from compost.
description	Electrochemically active (EA) biofilms were formed on metallic dimensionally stable anode-type electrode (DSA), embedded in garden compost and polarized at +0.50 V/SCE. Analysis of 16S rRNA gene libraries revealed that biofilms were heavily enriched in Deltaproteobacteria in comparison to control biofilms formed on non-polarized electrodes, which were preferentially composed of Gammaproteobacteria and Firmicutes. Among Deltaproteobacteria, sequences affiliated with Pelobacter and Geobacter genera were identified. A bacterial consortium was cultivated, in which 25 isolates were identified as Geobacter bremensis. Pure cultures of 4 different G. bremensis isolates gave higher current densities (1400 mA/m(2) on DSA, 2490 mA/m(2) on graphite) than the original multi-species biofilms (in average 300 mA/m(2) on DSA) and the G. bremensis DSM type strain (100-300 A/m(2) on DSA; 2485 mA/m(2) on graphite). FISH analysis confirmed that G. bremensis represented a minor fraction in the original EA biofilm, in which species related to Pelobacter genus were predominant. The Pelobacter type strain did not show EA capacity, which can explain the lower performance of the multi-species biofilms. These results stressed the great interest of extracting and culturing pure EA strains from wild EA biofilms to improve the current density provided by microbial anodes.
format	article
author	Olivier Nercessian Sandrine Parot Marie-Line Délia Alain Bergel Wafa Achouak
author_facet	Olivier Nercessian Sandrine Parot Marie-Line Délia Alain Bergel Wafa Achouak
author_sort	Olivier Nercessian
title	Harvesting electricity with Geobacter bremensis isolated from compost.
title_short	Harvesting electricity with Geobacter bremensis isolated from compost.
title_full	Harvesting electricity with Geobacter bremensis isolated from compost.
title_fullStr	Harvesting electricity with Geobacter bremensis isolated from compost.
title_full_unstemmed	Harvesting electricity with Geobacter bremensis isolated from compost.
title_sort	harvesting electricity with geobacter bremensis isolated from compost.
publisher	Public Library of Science (PLoS)
publishDate	2012
url	https://doaj.org/article/daa0fb4628ef412bb557cdeb52cf7cb3
work_keys_str_mv	AT oliviernercessian harvestingelectricitywithgeobacterbremensisisolatedfromcompost AT sandrineparot harvestingelectricitywithgeobacterbremensisisolatedfromcompost AT marielinedelia harvestingelectricitywithgeobacterbremensisisolatedfromcompost AT alainbergel harvestingelectricitywithgeobacterbremensisisolatedfromcompost AT wafaachouak harvestingelectricitywithgeobacterbremensisisolatedfromcompost
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Harvesting electricity with Geobacter bremensis isolated from compost.

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