ADAMTS9-AS1 Constrains Breast Cancer Cell Invasion and Proliferation via Sequestering miR-301b-3p

Objective: For determination of how ADAMTS9-AS1/miR-301b-3p/TGFBR2/JAK STAT signaling axis modulates progression of breast cancer cells.Methods: Target lncRNA was determined by differential analysis of breast cancer expression data and survival analysis. Differentially expressed miRNAs and target mR...

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Autores principales:	Junqing Chen, Ling Cheng, Weibin Zou, Rong Wang, Xiaojia Wang, Zhanhong Chen
Formato:	article
Lenguaje:	EN
Publicado:	Frontiers Media S.A. 2021
Materias:	ADAMTS9-AS1 miR-301b-3p TGFBR2 JAK STAT signaling pathway breast cancer Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/daa16a7cd153466bb1cd0711e392107e
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spelling	oai:doaj.org-article:daa16a7cd153466bb1cd0711e392107e2021-11-30T18:16:58ZADAMTS9-AS1 Constrains Breast Cancer Cell Invasion and Proliferation via Sequestering miR-301b-3p2296-634X10.3389/fcell.2021.719993https://doaj.org/article/daa16a7cd153466bb1cd0711e392107e2021-11-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fcell.2021.719993/fullhttps://doaj.org/toc/2296-634XObjective: For determination of how ADAMTS9-AS1/miR-301b-3p/TGFBR2/JAK STAT signaling axis modulates progression of breast cancer cells.Methods: Target lncRNA was determined by differential analysis of breast cancer expression data and survival analysis. Differentially expressed miRNAs and target mRNAs that had binding sites with target lncRNA were predicted. GSEA software was used to carry out pathway enrichment analysis for mRNAs. Binding of the researched genes were tested with RNA binding protein immunoprecipitation (RIP). How miR-301b-3p bound TGFBR2 mRNA was tested by dual-luciferase method. Transwell, colony formation, EdU approaches were employed for verification of invasion and proliferation of breast cancer cells in each treatment group.Results: Markedly inactivated ADAMTS9-AS1 in breast cancer pertained to patient’s prognosis. MiR-301b-3p was capable of binding TGFBR2/ADAMTS9-AS1. However, overexpression of ADAMTS9-AS1 stimulated miR-301b-3p binding ADAMTS9-AS1 and repressed miR-301b-3p binding TGFBR2 mRNA. ADAMTS9-AS1 interference enhanced cancer proliferation and invasion, facilitated levels of KI67, PCNA, MMP-9 and MMP-2, and activated the JAK STAT signaling pathway. While silencing miR-301b-3p reversed the effect of ADAMTS9-AS1 interference. In addition, TGFBR2 interference or restraining JAK STAT signaling counteracted the effect of ADAMTS9-AS1.Conclusion: ADAMTS9-AS1 could sequester miR-301b-3p to inhibit progression of breast cancer via TGFBR2/JAK STAT pathway. This study supplies a rationale for incremental apprehension of ADAMTS9-AS1 in breast cancer progression.Junqing ChenJunqing ChenLing ChengWeibin ZouWeibin ZouRong WangRong WangXiaojia WangXiaojia WangZhanhong ChenZhanhong ChenFrontiers Media S.A.articleADAMTS9-AS1miR-301b-3pTGFBR2JAK STAT signaling pathwaybreast cancerBiology (General)QH301-705.5ENFrontiers in Cell and Developmental Biology, Vol 9 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	ADAMTS9-AS1 miR-301b-3p TGFBR2 JAK STAT signaling pathway breast cancer Biology (General) QH301-705.5
spellingShingle	ADAMTS9-AS1 miR-301b-3p TGFBR2 JAK STAT signaling pathway breast cancer Biology (General) QH301-705.5 Junqing Chen Junqing Chen Ling Cheng Weibin Zou Weibin Zou Rong Wang Rong Wang Xiaojia Wang Xiaojia Wang Zhanhong Chen Zhanhong Chen ADAMTS9-AS1 Constrains Breast Cancer Cell Invasion and Proliferation via Sequestering miR-301b-3p
description	Objective: For determination of how ADAMTS9-AS1/miR-301b-3p/TGFBR2/JAK STAT signaling axis modulates progression of breast cancer cells.Methods: Target lncRNA was determined by differential analysis of breast cancer expression data and survival analysis. Differentially expressed miRNAs and target mRNAs that had binding sites with target lncRNA were predicted. GSEA software was used to carry out pathway enrichment analysis for mRNAs. Binding of the researched genes were tested with RNA binding protein immunoprecipitation (RIP). How miR-301b-3p bound TGFBR2 mRNA was tested by dual-luciferase method. Transwell, colony formation, EdU approaches were employed for verification of invasion and proliferation of breast cancer cells in each treatment group.Results: Markedly inactivated ADAMTS9-AS1 in breast cancer pertained to patient’s prognosis. MiR-301b-3p was capable of binding TGFBR2/ADAMTS9-AS1. However, overexpression of ADAMTS9-AS1 stimulated miR-301b-3p binding ADAMTS9-AS1 and repressed miR-301b-3p binding TGFBR2 mRNA. ADAMTS9-AS1 interference enhanced cancer proliferation and invasion, facilitated levels of KI67, PCNA, MMP-9 and MMP-2, and activated the JAK STAT signaling pathway. While silencing miR-301b-3p reversed the effect of ADAMTS9-AS1 interference. In addition, TGFBR2 interference or restraining JAK STAT signaling counteracted the effect of ADAMTS9-AS1.Conclusion: ADAMTS9-AS1 could sequester miR-301b-3p to inhibit progression of breast cancer via TGFBR2/JAK STAT pathway. This study supplies a rationale for incremental apprehension of ADAMTS9-AS1 in breast cancer progression.
format	article
author	Junqing Chen Junqing Chen Ling Cheng Weibin Zou Weibin Zou Rong Wang Rong Wang Xiaojia Wang Xiaojia Wang Zhanhong Chen Zhanhong Chen
author_facet	Junqing Chen Junqing Chen Ling Cheng Weibin Zou Weibin Zou Rong Wang Rong Wang Xiaojia Wang Xiaojia Wang Zhanhong Chen Zhanhong Chen
author_sort	Junqing Chen
title	ADAMTS9-AS1 Constrains Breast Cancer Cell Invasion and Proliferation via Sequestering miR-301b-3p
title_short	ADAMTS9-AS1 Constrains Breast Cancer Cell Invasion and Proliferation via Sequestering miR-301b-3p
title_full	ADAMTS9-AS1 Constrains Breast Cancer Cell Invasion and Proliferation via Sequestering miR-301b-3p
title_fullStr	ADAMTS9-AS1 Constrains Breast Cancer Cell Invasion and Proliferation via Sequestering miR-301b-3p
title_full_unstemmed	ADAMTS9-AS1 Constrains Breast Cancer Cell Invasion and Proliferation via Sequestering miR-301b-3p
title_sort	adamts9-as1 constrains breast cancer cell invasion and proliferation via sequestering mir-301b-3p
publisher	Frontiers Media S.A.
publishDate	2021
url	https://doaj.org/article/daa16a7cd153466bb1cd0711e392107e
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ADAMTS9-AS1 Constrains Breast Cancer Cell Invasion and Proliferation via Sequestering miR-301b-3p

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