Regulation of Signaling Pathways Involved in the Anti-proliferative and Apoptosis-inducing Effects of M22 against Non-small Cell Lung Adenocarcinoma A549 Cells
Abstract The compound 29-(4-methylpiperazine)-luepol (M22), a novel derivative of lupeol has shown anti-proliferative effects against the human non-small cell lung cancer A549 cell line. M22 showed significant anti-proliferative activity at 6.80 μM and increased accumulation of G1 cells and effectiv...
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Nature Portfolio
2018
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oai:doaj.org-article:daa7bdd317e7443f8394c982dfbde5ec2021-12-02T15:08:24ZRegulation of Signaling Pathways Involved in the Anti-proliferative and Apoptosis-inducing Effects of M22 against Non-small Cell Lung Adenocarcinoma A549 Cells10.1038/s41598-018-19368-02045-2322https://doaj.org/article/daa7bdd317e7443f8394c982dfbde5ec2018-01-01T00:00:00Zhttps://doi.org/10.1038/s41598-018-19368-0https://doaj.org/toc/2045-2322Abstract The compound 29-(4-methylpiperazine)-luepol (M22), a novel derivative of lupeol has shown anti-proliferative effects against the human non-small cell lung cancer A549 cell line. M22 showed significant anti-proliferative activity at 6.80 μM and increased accumulation of G1 cells and effectively suppressed expression of the G1 arrest-related genes cyclins D1 and E1, CDK2 and CDC25A. This was further confirmed by Western blotting demonstrating decreased cyclin D1 and CDC25A protein levels. Furthermore, M22 caused induction of apoptosis that downregulated the anti-apoptotic BCL-2 gene and increased expression of BAX, CASP3 and CASP9 as well as the APAF1 gene. The effect of caspase-induced apoptosis was confirmed by an increase in reactive oxygen species (ROS), loss of mitochondrial membrane potential (MMP). Taken together, our findings indicated that M22 possessed potent anti-proliferative and apoptotic activities.Yao YuanJiewei WuBailin LiJia NiuHaibo TanShengxiang QiuNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 8, Iss 1, Pp 1-9 (2018) |
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Medicine R Science Q |
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Medicine R Science Q Yao Yuan Jiewei Wu Bailin Li Jia Niu Haibo Tan Shengxiang Qiu Regulation of Signaling Pathways Involved in the Anti-proliferative and Apoptosis-inducing Effects of M22 against Non-small Cell Lung Adenocarcinoma A549 Cells |
| description |
Abstract The compound 29-(4-methylpiperazine)-luepol (M22), a novel derivative of lupeol has shown anti-proliferative effects against the human non-small cell lung cancer A549 cell line. M22 showed significant anti-proliferative activity at 6.80 μM and increased accumulation of G1 cells and effectively suppressed expression of the G1 arrest-related genes cyclins D1 and E1, CDK2 and CDC25A. This was further confirmed by Western blotting demonstrating decreased cyclin D1 and CDC25A protein levels. Furthermore, M22 caused induction of apoptosis that downregulated the anti-apoptotic BCL-2 gene and increased expression of BAX, CASP3 and CASP9 as well as the APAF1 gene. The effect of caspase-induced apoptosis was confirmed by an increase in reactive oxygen species (ROS), loss of mitochondrial membrane potential (MMP). Taken together, our findings indicated that M22 possessed potent anti-proliferative and apoptotic activities. |
| format |
article |
| author |
Yao Yuan Jiewei Wu Bailin Li Jia Niu Haibo Tan Shengxiang Qiu |
| author_facet |
Yao Yuan Jiewei Wu Bailin Li Jia Niu Haibo Tan Shengxiang Qiu |
| author_sort |
Yao Yuan |
| title |
Regulation of Signaling Pathways Involved in the Anti-proliferative and Apoptosis-inducing Effects of M22 against Non-small Cell Lung Adenocarcinoma A549 Cells |
| title_short |
Regulation of Signaling Pathways Involved in the Anti-proliferative and Apoptosis-inducing Effects of M22 against Non-small Cell Lung Adenocarcinoma A549 Cells |
| title_full |
Regulation of Signaling Pathways Involved in the Anti-proliferative and Apoptosis-inducing Effects of M22 against Non-small Cell Lung Adenocarcinoma A549 Cells |
| title_fullStr |
Regulation of Signaling Pathways Involved in the Anti-proliferative and Apoptosis-inducing Effects of M22 against Non-small Cell Lung Adenocarcinoma A549 Cells |
| title_full_unstemmed |
Regulation of Signaling Pathways Involved in the Anti-proliferative and Apoptosis-inducing Effects of M22 against Non-small Cell Lung Adenocarcinoma A549 Cells |
| title_sort |
regulation of signaling pathways involved in the anti-proliferative and apoptosis-inducing effects of m22 against non-small cell lung adenocarcinoma a549 cells |
| publisher |
Nature Portfolio |
| publishDate |
2018 |
| url |
https://doaj.org/article/daa7bdd317e7443f8394c982dfbde5ec |
| work_keys_str_mv |
AT yaoyuan regulationofsignalingpathwaysinvolvedintheantiproliferativeandapoptosisinducingeffectsofm22againstnonsmallcelllungadenocarcinomaa549cells AT jieweiwu regulationofsignalingpathwaysinvolvedintheantiproliferativeandapoptosisinducingeffectsofm22againstnonsmallcelllungadenocarcinomaa549cells AT bailinli regulationofsignalingpathwaysinvolvedintheantiproliferativeandapoptosisinducingeffectsofm22againstnonsmallcelllungadenocarcinomaa549cells AT jianiu regulationofsignalingpathwaysinvolvedintheantiproliferativeandapoptosisinducingeffectsofm22againstnonsmallcelllungadenocarcinomaa549cells AT haibotan regulationofsignalingpathwaysinvolvedintheantiproliferativeandapoptosisinducingeffectsofm22againstnonsmallcelllungadenocarcinomaa549cells AT shengxiangqiu regulationofsignalingpathwaysinvolvedintheantiproliferativeandapoptosisinducingeffectsofm22againstnonsmallcelllungadenocarcinomaa549cells |
| _version_ |
1718388153507643392 |