Definition of CRISPR Cas12a Trans-Cleavage Units to Facilitate CRISPR Diagnostics
The CRISPR diagnostic (CRISPR-Dx) technology that employs the trans-cleavage activities has shown great potential in diagnostic sensitivity, specificity, convenience, and portability, and has been recognized as the next-generation diagnostic methods. However, due to the lack of standardized definiti...
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Frontiers Media S.A.
2021
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oai:doaj.org-article:daa7bf3531534f83874bbea362f1d88a2021-12-01T13:47:18ZDefinition of CRISPR Cas12a Trans-Cleavage Units to Facilitate CRISPR Diagnostics1664-302X10.3389/fmicb.2021.766464https://doaj.org/article/daa7bf3531534f83874bbea362f1d88a2021-11-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fmicb.2021.766464/fullhttps://doaj.org/toc/1664-302XThe CRISPR diagnostic (CRISPR-Dx) technology that employs the trans-cleavage activities has shown great potential in diagnostic sensitivity, specificity, convenience, and portability, and has been recognized as the next-generation diagnostic methods. However, due to the lack of standardized definition of Cas trans-cleavage enzymatic units, it is difficult to standardize the present CRISPR-Dx systems, which have undoubtedly impeded the development of the CRISPR-Dx industry. To solve the problem, we here first systematically optimized the reaction systems for Cas12a, and then defined its trans-cleavage units (transU), which we believe will be of great importance and interest to researchers in both molecular diagnostic industry and basic research. Moreover, a simple protocol was provided to facilitate a step-by-step measurement of the Cas12a transU, which can also act as a reference for the definition of the transU for other Cas proteins.Hailong LvHailong LvJian WangJian ZhangYijian ChenYijian ChenLei YinDian JinDayong GuHuailong ZhaoYong XuJin WangJin WangFrontiers Media S.A.articleCRISPR diagnosticstrans-cleavageenzymatic unitsCas12transUMicrobiologyQR1-502ENFrontiers in Microbiology, Vol 12 (2021) |
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| collection |
DOAJ |
| language |
EN |
| topic |
CRISPR diagnostics trans-cleavage enzymatic units Cas12 transU Microbiology QR1-502 |
| spellingShingle |
CRISPR diagnostics trans-cleavage enzymatic units Cas12 transU Microbiology QR1-502 Hailong Lv Hailong Lv Jian Wang Jian Zhang Yijian Chen Yijian Chen Lei Yin Dian Jin Dayong Gu Huailong Zhao Yong Xu Jin Wang Jin Wang Definition of CRISPR Cas12a Trans-Cleavage Units to Facilitate CRISPR Diagnostics |
| description |
The CRISPR diagnostic (CRISPR-Dx) technology that employs the trans-cleavage activities has shown great potential in diagnostic sensitivity, specificity, convenience, and portability, and has been recognized as the next-generation diagnostic methods. However, due to the lack of standardized definition of Cas trans-cleavage enzymatic units, it is difficult to standardize the present CRISPR-Dx systems, which have undoubtedly impeded the development of the CRISPR-Dx industry. To solve the problem, we here first systematically optimized the reaction systems for Cas12a, and then defined its trans-cleavage units (transU), which we believe will be of great importance and interest to researchers in both molecular diagnostic industry and basic research. Moreover, a simple protocol was provided to facilitate a step-by-step measurement of the Cas12a transU, which can also act as a reference for the definition of the transU for other Cas proteins. |
| format |
article |
| author |
Hailong Lv Hailong Lv Jian Wang Jian Zhang Yijian Chen Yijian Chen Lei Yin Dian Jin Dayong Gu Huailong Zhao Yong Xu Jin Wang Jin Wang |
| author_facet |
Hailong Lv Hailong Lv Jian Wang Jian Zhang Yijian Chen Yijian Chen Lei Yin Dian Jin Dayong Gu Huailong Zhao Yong Xu Jin Wang Jin Wang |
| author_sort |
Hailong Lv |
| title |
Definition of CRISPR Cas12a Trans-Cleavage Units to Facilitate CRISPR Diagnostics |
| title_short |
Definition of CRISPR Cas12a Trans-Cleavage Units to Facilitate CRISPR Diagnostics |
| title_full |
Definition of CRISPR Cas12a Trans-Cleavage Units to Facilitate CRISPR Diagnostics |
| title_fullStr |
Definition of CRISPR Cas12a Trans-Cleavage Units to Facilitate CRISPR Diagnostics |
| title_full_unstemmed |
Definition of CRISPR Cas12a Trans-Cleavage Units to Facilitate CRISPR Diagnostics |
| title_sort |
definition of crispr cas12a trans-cleavage units to facilitate crispr diagnostics |
| publisher |
Frontiers Media S.A. |
| publishDate |
2021 |
| url |
https://doaj.org/article/daa7bf3531534f83874bbea362f1d88a |
| work_keys_str_mv |
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