Biomimetic Methacrylated Gelatin Hydrogel Loaded With Bone Marrow Mesenchymal Stem Cells for Bone Tissue Regeneration

Large-segment bone defect caused by trauma or tumor is one of the most challenging problems in orthopedic clinics. Biomimetic materials for bone tissue engineering have developed dramatically in the past few decades. The organic combination of biomimetic materials and stem cells offers new strategie...

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Autores principales:	Jun Li, Wenzhao Wang, Mingxin Li, Ping Song, Haoyuan Lei, Xingyu Gui, Changchun Zhou, Lei Liu
Formato:	article
Lenguaje:	EN
Publicado:	Frontiers Media S.A. 2021
Materias:	large segment bone defect GelMA hydrogel BMSCs ECM Biotechnology TP248.13-248.65
Acceso en línea:	https://doaj.org/article/daa90d5301d041d0b7603153456a04ce
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spelling	oai:doaj.org-article:daa90d5301d041d0b7603153456a04ce2021-12-02T11:54:08ZBiomimetic Methacrylated Gelatin Hydrogel Loaded With Bone Marrow Mesenchymal Stem Cells for Bone Tissue Regeneration2296-418510.3389/fbioe.2021.770049https://doaj.org/article/daa90d5301d041d0b7603153456a04ce2021-12-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fbioe.2021.770049/fullhttps://doaj.org/toc/2296-4185Large-segment bone defect caused by trauma or tumor is one of the most challenging problems in orthopedic clinics. Biomimetic materials for bone tissue engineering have developed dramatically in the past few decades. The organic combination of biomimetic materials and stem cells offers new strategies for tissue repair, and the fate of stem cells is closely related to their extracellular matrix (ECM) properties. In this study, a photocrosslinked biomimetic methacrylated gelatin (Bio-GelMA) hydrogel scaffold was prepared to simulate the physical structure and chemical composition of the natural bone extracellular matrix, providing a three-dimensional (3D) template and extracellular matrix microenvironment. Bone marrow mesenchymal stem cells (BMSCS) were encapsulated in Bio-GelMA scaffolds to examine the therapeutic effects of ECM-loaded cells in a 3D environment simulated for segmental bone defects. In vitro results showed that Bio-GelMA had good biocompatibility and sufficient mechanical properties (14.22kPa). A rat segmental bone defect model was constructed in vivo. The GelMA-BMSC suspension was added into the PDMS mold with the size of the bone defect and photocured as a scaffold. BMSC-loaded Bio-GelMA resulted in maximum and robust new bone formation compared with hydrogels alone and stem cell group. In conclusion, the bio-GelMA scaffold can be used as a cell carrier of BMSC to promote the repair of segmental bone defects and has great potential in future clinical applications.Jun LiWenzhao WangMingxin LiPing SongPing SongHaoyuan LeiHaoyuan LeiXingyu GuiXingyu GuiChangchun ZhouChangchun ZhouLei LiuFrontiers Media S.A.articlelarge segment bone defectGelMAhydrogelBMSCsECMBiotechnologyTP248.13-248.65ENFrontiers in Bioengineering and Biotechnology, Vol 9 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	large segment bone defect GelMA hydrogel BMSCs ECM Biotechnology TP248.13-248.65
spellingShingle	large segment bone defect GelMA hydrogel BMSCs ECM Biotechnology TP248.13-248.65 Jun Li Wenzhao Wang Mingxin Li Ping Song Ping Song Haoyuan Lei Haoyuan Lei Xingyu Gui Xingyu Gui Changchun Zhou Changchun Zhou Lei Liu Biomimetic Methacrylated Gelatin Hydrogel Loaded With Bone Marrow Mesenchymal Stem Cells for Bone Tissue Regeneration
description	Large-segment bone defect caused by trauma or tumor is one of the most challenging problems in orthopedic clinics. Biomimetic materials for bone tissue engineering have developed dramatically in the past few decades. The organic combination of biomimetic materials and stem cells offers new strategies for tissue repair, and the fate of stem cells is closely related to their extracellular matrix (ECM) properties. In this study, a photocrosslinked biomimetic methacrylated gelatin (Bio-GelMA) hydrogel scaffold was prepared to simulate the physical structure and chemical composition of the natural bone extracellular matrix, providing a three-dimensional (3D) template and extracellular matrix microenvironment. Bone marrow mesenchymal stem cells (BMSCS) were encapsulated in Bio-GelMA scaffolds to examine the therapeutic effects of ECM-loaded cells in a 3D environment simulated for segmental bone defects. In vitro results showed that Bio-GelMA had good biocompatibility and sufficient mechanical properties (14.22kPa). A rat segmental bone defect model was constructed in vivo. The GelMA-BMSC suspension was added into the PDMS mold with the size of the bone defect and photocured as a scaffold. BMSC-loaded Bio-GelMA resulted in maximum and robust new bone formation compared with hydrogels alone and stem cell group. In conclusion, the bio-GelMA scaffold can be used as a cell carrier of BMSC to promote the repair of segmental bone defects and has great potential in future clinical applications.
format	article
author	Jun Li Wenzhao Wang Mingxin Li Ping Song Ping Song Haoyuan Lei Haoyuan Lei Xingyu Gui Xingyu Gui Changchun Zhou Changchun Zhou Lei Liu
author_facet	Jun Li Wenzhao Wang Mingxin Li Ping Song Ping Song Haoyuan Lei Haoyuan Lei Xingyu Gui Xingyu Gui Changchun Zhou Changchun Zhou Lei Liu
author_sort	Jun Li
title	Biomimetic Methacrylated Gelatin Hydrogel Loaded With Bone Marrow Mesenchymal Stem Cells for Bone Tissue Regeneration
title_short	Biomimetic Methacrylated Gelatin Hydrogel Loaded With Bone Marrow Mesenchymal Stem Cells for Bone Tissue Regeneration
title_full	Biomimetic Methacrylated Gelatin Hydrogel Loaded With Bone Marrow Mesenchymal Stem Cells for Bone Tissue Regeneration
title_fullStr	Biomimetic Methacrylated Gelatin Hydrogel Loaded With Bone Marrow Mesenchymal Stem Cells for Bone Tissue Regeneration
title_full_unstemmed	Biomimetic Methacrylated Gelatin Hydrogel Loaded With Bone Marrow Mesenchymal Stem Cells for Bone Tissue Regeneration
title_sort	biomimetic methacrylated gelatin hydrogel loaded with bone marrow mesenchymal stem cells for bone tissue regeneration
publisher	Frontiers Media S.A.
publishDate	2021
url	https://doaj.org/article/daa90d5301d041d0b7603153456a04ce
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Biomimetic Methacrylated Gelatin Hydrogel Loaded With Bone Marrow Mesenchymal Stem Cells for Bone Tissue Regeneration

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