Photoactivatable RNAi for cancer gene therapy triggered by near-infrared-irradiated single-walled carbon nanotubes

Xueling Ren, Jing Lin, Xuefang Wang, Xiao Liu, Erjuan Meng, Rui Zhang, Yanxiao Sang, Zhenzhong Zhang Key Laboratory of Targeting Therapy and Diagnosis for Critical Diseases, School of Pharmaceutical Sciences, Zhengzhou University, Zhengzhou, People’s Republic of China Abstract: The effic...

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Autores principales:	Ren XL, Lin J, Wang XF, Liu X, Meng EJ, Zhang R, Sang YX, Zhang ZZ
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2017
Materias:	Near-infrared light response SWNT RNAi Hsp70B’ promoter Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/daa9a8ab09ee4871b36acbc1b93945e7
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spelling	oai:doaj.org-article:daa9a8ab09ee4871b36acbc1b93945e72021-12-02T08:20:23ZPhotoactivatable RNAi for cancer gene therapy triggered by near-infrared-irradiated single-walled carbon nanotubes1178-2013https://doaj.org/article/daa9a8ab09ee4871b36acbc1b93945e72017-10-01T00:00:00Zhttps://www.dovepress.com/photoactivatable-rnai-for-cancer-gene-therapy-triggered-by-near-infrar-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Xueling Ren, Jing Lin, Xuefang Wang, Xiao Liu, Erjuan Meng, Rui Zhang, Yanxiao Sang, Zhenzhong Zhang Key Laboratory of Targeting Therapy and Diagnosis for Critical Diseases, School of Pharmaceutical Sciences, Zhengzhou University, Zhengzhou, People’s Republic of China Abstract: The efficacy of RNA interference (RNAi)-based cancer gene therapy is limited by its unexpected side effects, thus necessitating a strategy to precisely trigger conditional gene knockdown. In this study, we engineered a novel photoactivatable RNAi system, named as polyetherimide-modified single-wall carbon nanotube (PEI-SWNT)/pHSP-shT, that enables optogenetic control of targeted gene suppression in tumor cells. PEI-SWNT/pHSP-shT comprises a stimulus-responsive nanocarrier (PEI-SWNT), and an Hsp70B'-promoter-driven RNAi vector (pHSP-shT). In response to near-infrared (NIR) light irradiation, heating of PEI-SWNT in breast MCF-7 cells triggered gene knockdown targeting human telomerase reverse transcriptase through RNAi, with the gene-knockdown activity capable of being switched off by extinguishing the NIR. Furthermore, we demonstrated that the photoactivatable RNAi system exhibited higher antitumor activity by combining gene therapy and photothermal therapy, both in vitro and in vivo. Optogenetic control of RNAi based on an NIR-activated nanocarrier will potentially facilitate improved understanding of molecular-targeted gene therapy in human malignant tumors. Keywords: near-infrared light response, SWNT, RNAi, Hsp70B' promoterRen XLLin JWang XFLiu XMeng EJZhang RSang YXZhang ZZDove Medical PressarticleNear-infrared light responseSWNTRNAiHsp70B’ promoterMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 12, Pp 7885-7896 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Near-infrared light response SWNT RNAi Hsp70B’ promoter Medicine (General) R5-920
spellingShingle	Near-infrared light response SWNT RNAi Hsp70B’ promoter Medicine (General) R5-920 Ren XL Lin J Wang XF Liu X Meng EJ Zhang R Sang YX Zhang ZZ Photoactivatable RNAi for cancer gene therapy triggered by near-infrared-irradiated single-walled carbon nanotubes
description	Xueling Ren, Jing Lin, Xuefang Wang, Xiao Liu, Erjuan Meng, Rui Zhang, Yanxiao Sang, Zhenzhong Zhang Key Laboratory of Targeting Therapy and Diagnosis for Critical Diseases, School of Pharmaceutical Sciences, Zhengzhou University, Zhengzhou, People’s Republic of China Abstract: The efficacy of RNA interference (RNAi)-based cancer gene therapy is limited by its unexpected side effects, thus necessitating a strategy to precisely trigger conditional gene knockdown. In this study, we engineered a novel photoactivatable RNAi system, named as polyetherimide-modified single-wall carbon nanotube (PEI-SWNT)/pHSP-shT, that enables optogenetic control of targeted gene suppression in tumor cells. PEI-SWNT/pHSP-shT comprises a stimulus-responsive nanocarrier (PEI-SWNT), and an Hsp70B'-promoter-driven RNAi vector (pHSP-shT). In response to near-infrared (NIR) light irradiation, heating of PEI-SWNT in breast MCF-7 cells triggered gene knockdown targeting human telomerase reverse transcriptase through RNAi, with the gene-knockdown activity capable of being switched off by extinguishing the NIR. Furthermore, we demonstrated that the photoactivatable RNAi system exhibited higher antitumor activity by combining gene therapy and photothermal therapy, both in vitro and in vivo. Optogenetic control of RNAi based on an NIR-activated nanocarrier will potentially facilitate improved understanding of molecular-targeted gene therapy in human malignant tumors. Keywords: near-infrared light response, SWNT, RNAi, Hsp70B' promoter
format	article
author	Ren XL Lin J Wang XF Liu X Meng EJ Zhang R Sang YX Zhang ZZ
author_facet	Ren XL Lin J Wang XF Liu X Meng EJ Zhang R Sang YX Zhang ZZ
author_sort	Ren XL
title	Photoactivatable RNAi for cancer gene therapy triggered by near-infrared-irradiated single-walled carbon nanotubes
title_short	Photoactivatable RNAi for cancer gene therapy triggered by near-infrared-irradiated single-walled carbon nanotubes
title_full	Photoactivatable RNAi for cancer gene therapy triggered by near-infrared-irradiated single-walled carbon nanotubes
title_fullStr	Photoactivatable RNAi for cancer gene therapy triggered by near-infrared-irradiated single-walled carbon nanotubes
title_full_unstemmed	Photoactivatable RNAi for cancer gene therapy triggered by near-infrared-irradiated single-walled carbon nanotubes
title_sort	photoactivatable rnai for cancer gene therapy triggered by near-infrared-irradiated single-walled carbon nanotubes
publisher	Dove Medical Press
publishDate	2017
url	https://doaj.org/article/daa9a8ab09ee4871b36acbc1b93945e7
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Photoactivatable RNAi for cancer gene therapy triggered by near-infrared-irradiated single-walled carbon nanotubes

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