Well-Orientation Strategy Biosynthesis of Cefuroxime-Silver Nanoantibiotic for Reinforced Biodentine™ and Its Dental Application against Streptococcus mutans

Dental caries results from the bacterial pathogen Streptococcus mutans (S. mutans) and is the maximum critical reason for caries formation. Consequently, the present study aims to evaluate the antibacterial activity of a newly synthesized nanoantibiotic–Biodenti...

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Autores principales:	Sanaa M. F. Gad El-Rab, Amal A. Ashour, Sakeenabi Basha, Amal Ahmed Alyamani, Nayef H. Felemban, Enas Tawfik Enan
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	antimicrobial activity Biodentine™ Cefuroxime-ROE-AgNPs <i>Rosmarinus officinalis</i> extract Organic chemistry QD241-441
Acceso en línea:	https://doaj.org/article/daaa7fad6758421098eac0455ed069a7
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spelling	oai:doaj.org-article:daaa7fad6758421098eac0455ed069a72021-11-25T18:27:35ZWell-Orientation Strategy Biosynthesis of Cefuroxime-Silver Nanoantibiotic for Reinforced Biodentine™ and Its Dental Application against <i>Streptococcus mutans</i>10.3390/molecules262268321420-3049https://doaj.org/article/daaa7fad6758421098eac0455ed069a72021-11-01T00:00:00Zhttps://www.mdpi.com/1420-3049/26/22/6832https://doaj.org/toc/1420-3049Dental caries results from the bacterial pathogen <i>Streptococcus mutans</i> (<i>S. mutans</i>) and is the maximum critical reason for caries formation. Consequently, the present study aims to evaluate the antibacterial activity of a newly synthesized nanoantibiotic–Biodentine formulation. The silver nanoparticles (ROE-AgNPs) were biosynthesized from the usage of <i>Rosmarinus </i><i>officinalis</i> L. extract (ROE) and conjugated with cefuroxime to form Cefuroxime-ROE-AgNPs. Using Biodentine™ (BIOD), five groups of dental materials were prepared, in which Group A included conventional BIOD, Group B included BIOD with ROE-AgNPs, Groups C and D included BIOD with Cefuroxime-ROE-AgNPs at concentrations of 0.5% and 1.5% cefuroxime, respectively, and Group E included BIOD with 1.5% cefuroxime. The synthesized ROE-AgNPs or Cefuroxime-ROE-AgNPs were characterized for conjugating efficiency, morphology, particle size, and in vitro release. Minimum inhibitory concentration (MIC) of the cefuroxime, ROE-AgNPs, and Cefuroxime-ROE-AgNPs were additionally evaluated against cefuroxime resistant <i>S. mutans</i>, which furthered antibacterial efficacy of the five groups of dental materials. The UV-Visible spectrum showed the ROE-AgNPs or Cefuroxime-ROE-AgNPs peaks and their formation displayed through transmission electron microscopy (TEM), X-ray diffraction (XRD) pattern, and Fourier transforms infrared (FTIR) analysis. The end result of Cefuroxime-ROE-AgNPs showed conjugating efficiency up to 79%. Cefuroxime-ROE-AgNPs displayed the highest antibacterial efficacy against <i>S. mutans</i> as compared to cefuroxime or ROE-AgNPs alone. Moreover, the MIC of ROE-AgNPs and Cefuroxime-ROE-AgNPs was detected against <i>S. mutans</i> to be 25 and 8.5 μg/mL, respectively. Consequently, Cefuroxime-ROE-AgNPs displayed that a decrease in the MIC reached to more than three-fold less than MIC of ROE-AgNPs on the tested strain. Moreover, Cefuroxime-ROE-AgNPs/BIOD was employed as a novel dental material that showed maximum antimicrobial activity. Groups C and D of novel materials showed inhibitory zones of 19 and 26 mm, respectively, against <i>S. mutans</i> and showed high antimicrobial rates of 85.78% and 91.17%, respectively. These data reinforce the utility of conjugating cefuroxime with ROE-AgNPs to retrieve its efficiency against resistant <i>S. mutant</i>. Moreover, the nanoantibiotic delivered an advantageous antibacterial effect to BIOD, and this may open the door for future conjugation therapy of dental materials against bacteria that cause dental caries.Sanaa M. F. Gad El-RabAmal A. AshourSakeenabi BashaAmal Ahmed AlyamaniNayef H. FelembanEnas Tawfik EnanMDPI AGarticleantimicrobial activityBiodentine™Cefuroxime-ROE-AgNPs<i>Rosmarinus officinalis</i> extractOrganic chemistryQD241-441ENMolecules, Vol 26, Iss 6832, p 6832 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	antimicrobial activity Biodentine™ Cefuroxime-ROE-AgNPs <i>Rosmarinus officinalis</i> extract Organic chemistry QD241-441
spellingShingle	antimicrobial activity Biodentine™ Cefuroxime-ROE-AgNPs <i>Rosmarinus officinalis</i> extract Organic chemistry QD241-441 Sanaa M. F. Gad El-Rab Amal A. Ashour Sakeenabi Basha Amal Ahmed Alyamani Nayef H. Felemban Enas Tawfik Enan Well-Orientation Strategy Biosynthesis of Cefuroxime-Silver Nanoantibiotic for Reinforced Biodentine™ and Its Dental Application against <i>Streptococcus mutans</i>
description	Dental caries results from the bacterial pathogen <i>Streptococcus mutans</i> (<i>S. mutans</i>) and is the maximum critical reason for caries formation. Consequently, the present study aims to evaluate the antibacterial activity of a newly synthesized nanoantibiotic–Biodentine formulation. The silver nanoparticles (ROE-AgNPs) were biosynthesized from the usage of <i>Rosmarinus </i><i>officinalis</i> L. extract (ROE) and conjugated with cefuroxime to form Cefuroxime-ROE-AgNPs. Using Biodentine™ (BIOD), five groups of dental materials were prepared, in which Group A included conventional BIOD, Group B included BIOD with ROE-AgNPs, Groups C and D included BIOD with Cefuroxime-ROE-AgNPs at concentrations of 0.5% and 1.5% cefuroxime, respectively, and Group E included BIOD with 1.5% cefuroxime. The synthesized ROE-AgNPs or Cefuroxime-ROE-AgNPs were characterized for conjugating efficiency, morphology, particle size, and in vitro release. Minimum inhibitory concentration (MIC) of the cefuroxime, ROE-AgNPs, and Cefuroxime-ROE-AgNPs were additionally evaluated against cefuroxime resistant <i>S. mutans</i>, which furthered antibacterial efficacy of the five groups of dental materials. The UV-Visible spectrum showed the ROE-AgNPs or Cefuroxime-ROE-AgNPs peaks and their formation displayed through transmission electron microscopy (TEM), X-ray diffraction (XRD) pattern, and Fourier transforms infrared (FTIR) analysis. The end result of Cefuroxime-ROE-AgNPs showed conjugating efficiency up to 79%. Cefuroxime-ROE-AgNPs displayed the highest antibacterial efficacy against <i>S. mutans</i> as compared to cefuroxime or ROE-AgNPs alone. Moreover, the MIC of ROE-AgNPs and Cefuroxime-ROE-AgNPs was detected against <i>S. mutans</i> to be 25 and 8.5 μg/mL, respectively. Consequently, Cefuroxime-ROE-AgNPs displayed that a decrease in the MIC reached to more than three-fold less than MIC of ROE-AgNPs on the tested strain. Moreover, Cefuroxime-ROE-AgNPs/BIOD was employed as a novel dental material that showed maximum antimicrobial activity. Groups C and D of novel materials showed inhibitory zones of 19 and 26 mm, respectively, against <i>S. mutans</i> and showed high antimicrobial rates of 85.78% and 91.17%, respectively. These data reinforce the utility of conjugating cefuroxime with ROE-AgNPs to retrieve its efficiency against resistant <i>S. mutant</i>. Moreover, the nanoantibiotic delivered an advantageous antibacterial effect to BIOD, and this may open the door for future conjugation therapy of dental materials against bacteria that cause dental caries.
format	article
author	Sanaa M. F. Gad El-Rab Amal A. Ashour Sakeenabi Basha Amal Ahmed Alyamani Nayef H. Felemban Enas Tawfik Enan
author_facet	Sanaa M. F. Gad El-Rab Amal A. Ashour Sakeenabi Basha Amal Ahmed Alyamani Nayef H. Felemban Enas Tawfik Enan
author_sort	Sanaa M. F. Gad El-Rab
title	Well-Orientation Strategy Biosynthesis of Cefuroxime-Silver Nanoantibiotic for Reinforced Biodentine™ and Its Dental Application against <i>Streptococcus mutans</i>
title_short	Well-Orientation Strategy Biosynthesis of Cefuroxime-Silver Nanoantibiotic for Reinforced Biodentine™ and Its Dental Application against <i>Streptococcus mutans</i>
title_full	Well-Orientation Strategy Biosynthesis of Cefuroxime-Silver Nanoantibiotic for Reinforced Biodentine™ and Its Dental Application against <i>Streptococcus mutans</i>
title_fullStr	Well-Orientation Strategy Biosynthesis of Cefuroxime-Silver Nanoantibiotic for Reinforced Biodentine™ and Its Dental Application against <i>Streptococcus mutans</i>
title_full_unstemmed	Well-Orientation Strategy Biosynthesis of Cefuroxime-Silver Nanoantibiotic for Reinforced Biodentine™ and Its Dental Application against <i>Streptococcus mutans</i>
title_sort	well-orientation strategy biosynthesis of cefuroxime-silver nanoantibiotic for reinforced biodentine™ and its dental application against <i>streptococcus mutans</i>
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/daaa7fad6758421098eac0455ed069a7
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Well-Orientation Strategy Biosynthesis of Cefuroxime-Silver Nanoantibiotic for Reinforced Biodentine™ and Its Dental Application against <i>Streptococcus mutans</i>

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