Identification of the circRNA-miRNA-mRNA Regulatory Network in Bladder Cancer by Bioinformatics Analysis

In recent years, increasing evidence shows that circular RNA (circRNA) disorder is closely related to tumorigenesis and cancer progression. However, the regulatory functions of most circRNAs in bladder cancer (BCa) remain unclear. This study was aimed at exploring the molecular regulatory mechanism...

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Autores principales:	Jiancheng Lv, Ping-an Chang, Xin Li, Xiao Yang, Jie Han, Hao Yu, Zijian Zhou, Haiwei Yang, Pengchao Li, Jiexiu Zhang, Qiang Lu
Formato:	article
Lenguaje:	EN
Publicado:	Hindawi Limited 2021
Materias:	Genetics QH426-470
Acceso en línea:	https://doaj.org/article/daac8240db67402d89877aace1801625
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spelling	oai:doaj.org-article:daac8240db67402d89877aace18016252021-11-29T00:56:36ZIdentification of the circRNA-miRNA-mRNA Regulatory Network in Bladder Cancer by Bioinformatics Analysis2314-437810.1155/2021/9935986https://doaj.org/article/daac8240db67402d89877aace18016252021-01-01T00:00:00Zhttp://dx.doi.org/10.1155/2021/9935986https://doaj.org/toc/2314-4378In recent years, increasing evidence shows that circular RNA (circRNA) disorder is closely related to tumorigenesis and cancer progression. However, the regulatory functions of most circRNAs in bladder cancer (BCa) remain unclear. This study was aimed at exploring the molecular regulatory mechanism of circRNAs in BCa. We obtained four datasets of circRNA, microRNA (miRNA), and messenger (mRNA) expression profiles from the Gene Expression Omnibus and The Cancer Genome Atlas microarray databases and identified 434, 367, and 4799/4841 differentially expressed circRNAs, miRNAs, and mRNAs, respectively. With these differentially expressed RNAs, we established a circRNA-miRNA-mRNA targeted interaction network. A total of 18, 24, and 51 central circRNAs, miRNAs, and mRNAs were identified, respectively. Among them, the top 10 mRNAs that had high connectivity with other circRNAs and miRNAs were regarded as hub genes. We detected the expression levels of these 10 mRNAs in 16 pairs of BCa tissues and adjacent normal tissues through quantitative real-time polymerase chain reaction. The differentially expressed mRNAs and central mRNAs were enriched in the processes and pathways that are associated with the growth, differentiation, proliferation, and apoptosis of tumor cells. The outstanding genes (CDCA4, GATA6, LATS2, RHOB, ZBTB4, and ZFPM2) also interacted with numerous drugs, indicating their potency as biomarkers and drug targets. The findings of this study provide a deep understanding of the circRNA-related competitive endogenous RNA regulatory mechanism in BCa pathogenesis.Jiancheng LvPing-an ChangXin LiXiao YangJie HanHao YuZijian ZhouHaiwei YangPengchao LiJiexiu ZhangQiang LuHindawi LimitedarticleGeneticsQH426-470ENInternational Journal of Genomics, Vol 2021 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Genetics QH426-470
spellingShingle	Genetics QH426-470 Jiancheng Lv Ping-an Chang Xin Li Xiao Yang Jie Han Hao Yu Zijian Zhou Haiwei Yang Pengchao Li Jiexiu Zhang Qiang Lu Identification of the circRNA-miRNA-mRNA Regulatory Network in Bladder Cancer by Bioinformatics Analysis
description	In recent years, increasing evidence shows that circular RNA (circRNA) disorder is closely related to tumorigenesis and cancer progression. However, the regulatory functions of most circRNAs in bladder cancer (BCa) remain unclear. This study was aimed at exploring the molecular regulatory mechanism of circRNAs in BCa. We obtained four datasets of circRNA, microRNA (miRNA), and messenger (mRNA) expression profiles from the Gene Expression Omnibus and The Cancer Genome Atlas microarray databases and identified 434, 367, and 4799/4841 differentially expressed circRNAs, miRNAs, and mRNAs, respectively. With these differentially expressed RNAs, we established a circRNA-miRNA-mRNA targeted interaction network. A total of 18, 24, and 51 central circRNAs, miRNAs, and mRNAs were identified, respectively. Among them, the top 10 mRNAs that had high connectivity with other circRNAs and miRNAs were regarded as hub genes. We detected the expression levels of these 10 mRNAs in 16 pairs of BCa tissues and adjacent normal tissues through quantitative real-time polymerase chain reaction. The differentially expressed mRNAs and central mRNAs were enriched in the processes and pathways that are associated with the growth, differentiation, proliferation, and apoptosis of tumor cells. The outstanding genes (CDCA4, GATA6, LATS2, RHOB, ZBTB4, and ZFPM2) also interacted with numerous drugs, indicating their potency as biomarkers and drug targets. The findings of this study provide a deep understanding of the circRNA-related competitive endogenous RNA regulatory mechanism in BCa pathogenesis.
format	article
author	Jiancheng Lv Ping-an Chang Xin Li Xiao Yang Jie Han Hao Yu Zijian Zhou Haiwei Yang Pengchao Li Jiexiu Zhang Qiang Lu
author_facet	Jiancheng Lv Ping-an Chang Xin Li Xiao Yang Jie Han Hao Yu Zijian Zhou Haiwei Yang Pengchao Li Jiexiu Zhang Qiang Lu
author_sort	Jiancheng Lv
title	Identification of the circRNA-miRNA-mRNA Regulatory Network in Bladder Cancer by Bioinformatics Analysis
title_short	Identification of the circRNA-miRNA-mRNA Regulatory Network in Bladder Cancer by Bioinformatics Analysis
title_full	Identification of the circRNA-miRNA-mRNA Regulatory Network in Bladder Cancer by Bioinformatics Analysis
title_fullStr	Identification of the circRNA-miRNA-mRNA Regulatory Network in Bladder Cancer by Bioinformatics Analysis
title_full_unstemmed	Identification of the circRNA-miRNA-mRNA Regulatory Network in Bladder Cancer by Bioinformatics Analysis
title_sort	identification of the circrna-mirna-mrna regulatory network in bladder cancer by bioinformatics analysis
publisher	Hindawi Limited
publishDate	2021
url	https://doaj.org/article/daac8240db67402d89877aace1801625
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Identification of the circRNA-miRNA-mRNA Regulatory Network in Bladder Cancer by Bioinformatics Analysis

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