Cerium oxide nanoparticles protect rodent lungs from hypobaric hypoxia-induced oxidative stress and inflammation

Aditya Arya,1 Niroj Kumar Sethy,1 Sushil Kumar Singh,2 Mainak Das,3 Kalpana Bhargava1 1Peptide and Proteomics Division, Defence Institute of Physiology and Allied Sciences, Defence Research and Development Organization, Delhi, 2Functional Materials Division, Solid State Physics Laboratory, Defence R...

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Autores principales: Arya A, Sethy NK, Singh SK, Das M, Bhargava K
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Publicado: Dove Medical Press 2013
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spelling oai:doaj.org-article:daad6091197d47bb8e437742747b98a02021-12-02T02:04:17ZCerium oxide nanoparticles protect rodent lungs from hypobaric hypoxia-induced oxidative stress and inflammation1176-91141178-2013https://doaj.org/article/daad6091197d47bb8e437742747b98a02013-11-01T00:00:00Zhttp://www.dovepress.com/cerium-oxide-nanoparticles-protect-rodent-lungs-from-hypobaric-hypoxia-a15041https://doaj.org/toc/1176-9114https://doaj.org/toc/1178-2013Aditya Arya,1 Niroj Kumar Sethy,1 Sushil Kumar Singh,2 Mainak Das,3 Kalpana Bhargava1 1Peptide and Proteomics Division, Defence Institute of Physiology and Allied Sciences, Defence Research and Development Organization, Delhi, 2Functional Materials Division, Solid State Physics Laboratory, Defence Research and Development Organization, Delhi, 3Biological Science and Bioengineering, Indian Institute of Technology, Kanpur, Uttar Pradesh, India Background: Cerium oxide nanoparticles (nanoceria) are effective at quenching reactive oxygen species (ROS) in cell culture and animal models. Although nanoceria reportedly deposit in lungs, their efficacy in conferring lung protection during oxidative stress remains unexplored. Thus, the study evaluated the protective efficacy of nanoceria in rat lung tissue during hypobaric hypoxia. Methods: A total of 48 animals were randomly divided into four equal groups (control [C], nanoceria treated [T], hypoxia [H], and nanoceria treated plus hypoxia [T+H]). Animals were injected intraperitoneally with either a dose of 0.5 µg/kg body weight/week of nanoceria (T and T+H groups) or vehicle (C and H groups) for 5 weeks. After the final dose, H and T+H animals were challenged with hypobaric hypoxia, while C and T animals were maintained at normoxia. Lungs were isolated and homogenate was obtained for analysis of ROS, lipid peroxidation, glutathione, protein carbonylation, and 4-hydroxynonenal-adduct formation. Plasma was used for estimating major inflammatory cytokines using enzyme-linked immunosorbent assay. Intact lung tissues were fixed and both transmission electron microscopy and histopathological examinations were carried out separately for detecting internalization of nanoparticles as well as altered lung morphology. Results: Spherical nanoceria of 7–10 nm diameter were synthesized using a microemulsion method, and the lung protective efficacy of the nanoceria evaluated during hypobaric hypoxia. With repeated intraperitoneal injections of low micromole concentration, we successfully localized the nanoceria in rodent lung without any inflammatory response. The lung-deposited nanoceria limited ROS formation, lipid peroxidation, and glutathione oxidation, and prevented oxidative protein modifications like nitration and carbonyl formation during hypobaric hypoxia. We also observed reduced lung inflammation in the nanoceria-injected lungs, supporting the anti-inflammatory properties of nanoceria. Conclusion: Cumulatively, these results suggest nanoceria deposit in lungs, confer protection by quenching noxious free radicals during hypobaric hypoxia, and do not evoke any inflammatory response. Keywords: nanoceria, high altitude, nanomedicineArya ASethy NKSingh SKDas MBhargava KDove Medical PressarticleMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2013, Iss Issue 1, Pp 4507-4520 (2013)
institution DOAJ
collection DOAJ
language EN
topic Medicine (General)
R5-920
spellingShingle Medicine (General)
R5-920
Arya A
Sethy NK
Singh SK
Das M
Bhargava K
Cerium oxide nanoparticles protect rodent lungs from hypobaric hypoxia-induced oxidative stress and inflammation
description Aditya Arya,1 Niroj Kumar Sethy,1 Sushil Kumar Singh,2 Mainak Das,3 Kalpana Bhargava1 1Peptide and Proteomics Division, Defence Institute of Physiology and Allied Sciences, Defence Research and Development Organization, Delhi, 2Functional Materials Division, Solid State Physics Laboratory, Defence Research and Development Organization, Delhi, 3Biological Science and Bioengineering, Indian Institute of Technology, Kanpur, Uttar Pradesh, India Background: Cerium oxide nanoparticles (nanoceria) are effective at quenching reactive oxygen species (ROS) in cell culture and animal models. Although nanoceria reportedly deposit in lungs, their efficacy in conferring lung protection during oxidative stress remains unexplored. Thus, the study evaluated the protective efficacy of nanoceria in rat lung tissue during hypobaric hypoxia. Methods: A total of 48 animals were randomly divided into four equal groups (control [C], nanoceria treated [T], hypoxia [H], and nanoceria treated plus hypoxia [T+H]). Animals were injected intraperitoneally with either a dose of 0.5 µg/kg body weight/week of nanoceria (T and T+H groups) or vehicle (C and H groups) for 5 weeks. After the final dose, H and T+H animals were challenged with hypobaric hypoxia, while C and T animals were maintained at normoxia. Lungs were isolated and homogenate was obtained for analysis of ROS, lipid peroxidation, glutathione, protein carbonylation, and 4-hydroxynonenal-adduct formation. Plasma was used for estimating major inflammatory cytokines using enzyme-linked immunosorbent assay. Intact lung tissues were fixed and both transmission electron microscopy and histopathological examinations were carried out separately for detecting internalization of nanoparticles as well as altered lung morphology. Results: Spherical nanoceria of 7–10 nm diameter were synthesized using a microemulsion method, and the lung protective efficacy of the nanoceria evaluated during hypobaric hypoxia. With repeated intraperitoneal injections of low micromole concentration, we successfully localized the nanoceria in rodent lung without any inflammatory response. The lung-deposited nanoceria limited ROS formation, lipid peroxidation, and glutathione oxidation, and prevented oxidative protein modifications like nitration and carbonyl formation during hypobaric hypoxia. We also observed reduced lung inflammation in the nanoceria-injected lungs, supporting the anti-inflammatory properties of nanoceria. Conclusion: Cumulatively, these results suggest nanoceria deposit in lungs, confer protection by quenching noxious free radicals during hypobaric hypoxia, and do not evoke any inflammatory response. Keywords: nanoceria, high altitude, nanomedicine
format article
author Arya A
Sethy NK
Singh SK
Das M
Bhargava K
author_facet Arya A
Sethy NK
Singh SK
Das M
Bhargava K
author_sort Arya A
title Cerium oxide nanoparticles protect rodent lungs from hypobaric hypoxia-induced oxidative stress and inflammation
title_short Cerium oxide nanoparticles protect rodent lungs from hypobaric hypoxia-induced oxidative stress and inflammation
title_full Cerium oxide nanoparticles protect rodent lungs from hypobaric hypoxia-induced oxidative stress and inflammation
title_fullStr Cerium oxide nanoparticles protect rodent lungs from hypobaric hypoxia-induced oxidative stress and inflammation
title_full_unstemmed Cerium oxide nanoparticles protect rodent lungs from hypobaric hypoxia-induced oxidative stress and inflammation
title_sort cerium oxide nanoparticles protect rodent lungs from hypobaric hypoxia-induced oxidative stress and inflammation
publisher Dove Medical Press
publishDate 2013
url https://doaj.org/article/daad6091197d47bb8e437742747b98a0
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AT sethynk ceriumoxidenanoparticlesprotectrodentlungsfromhypobarichypoxiainducedoxidativestressandinflammation
AT singhsk ceriumoxidenanoparticlesprotectrodentlungsfromhypobarichypoxiainducedoxidativestressandinflammation
AT dasm ceriumoxidenanoparticlesprotectrodentlungsfromhypobarichypoxiainducedoxidativestressandinflammation
AT bhargavak ceriumoxidenanoparticlesprotectrodentlungsfromhypobarichypoxiainducedoxidativestressandinflammation
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