Development of a multi-well-chip for studying 2D and 3D tumor cell migration and spheroid growth in electrical fields

Endogenous electrical fields play an important role in various physiological and pathological events. Yet the effects of electrical cues on processes such as wound healing, tumor development or metastasis are still rarely investigated, though it is known that direct current electrical fields can alt...

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Autores principales:	Kemkemer Ralf, Naggay Benjamin K., Schmidt Tobias B., Ende Karen
Formato:	article
Lenguaje:	EN
Publicado:	De Gruyter 2020
Materias:	electrical field galvanotaxis electrotaxis cell motility tumor cells Medicine R
Acceso en línea:	https://doaj.org/article/dab6ee680b964b8092912ac2b3cc54bd
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spelling	oai:doaj.org-article:dab6ee680b964b8092912ac2b3cc54bd2021-12-05T14:10:42ZDevelopment of a multi-well-chip for studying 2D and 3D tumor cell migration and spheroid growth in electrical fields2364-550410.1515/cdbme-2020-3042https://doaj.org/article/dab6ee680b964b8092912ac2b3cc54bd2020-09-01T00:00:00Zhttps://doi.org/10.1515/cdbme-2020-3042https://doaj.org/toc/2364-5504Endogenous electrical fields play an important role in various physiological and pathological events. Yet the effects of electrical cues on processes such as wound healing, tumor development or metastasis are still rarely investigated, though it is known that direct current electrical fields can alter cell migration or proliferation in vitro. Several 2D experimental models for studying cell responses to direct current electrical fields have been presented and characterized but suitable experimental models for electrotaxis studies in 3D are rare. Here we present a novel, easy-to-produce, multi-well-based galvanotactic-chamber for the use in 2D and 3D cell experiments for investigations on the influence of electrical fields on tumor cell migration and tumor spheroid growth. Our presented system allows the simultaneous application of electrical field to cells in four chambers, either cultured on the bottom of the culture-plate (2D) or embedded in hydrogel filled channels (3D). The set-up is also suitable for, live-cell-imaging. Validation tests show stable electrical fields and high cell viabilities inside the channel. Tumor spheroids of various diameters can be exposed to direct current electrical fields up to one week.Kemkemer RalfNaggay Benjamin K.Schmidt Tobias B.Ende KarenDe Gruyterarticleelectrical fieldgalvanotaxiselectrotaxiscell motilitytumor cellsMedicineRENCurrent Directions in Biomedical Engineering, Vol 6, Iss 3, Pp 164-167 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	electrical field galvanotaxis electrotaxis cell motility tumor cells Medicine R
spellingShingle	electrical field galvanotaxis electrotaxis cell motility tumor cells Medicine R Kemkemer Ralf Naggay Benjamin K. Schmidt Tobias B. Ende Karen Development of a multi-well-chip for studying 2D and 3D tumor cell migration and spheroid growth in electrical fields
description	Endogenous electrical fields play an important role in various physiological and pathological events. Yet the effects of electrical cues on processes such as wound healing, tumor development or metastasis are still rarely investigated, though it is known that direct current electrical fields can alter cell migration or proliferation in vitro. Several 2D experimental models for studying cell responses to direct current electrical fields have been presented and characterized but suitable experimental models for electrotaxis studies in 3D are rare. Here we present a novel, easy-to-produce, multi-well-based galvanotactic-chamber for the use in 2D and 3D cell experiments for investigations on the influence of electrical fields on tumor cell migration and tumor spheroid growth. Our presented system allows the simultaneous application of electrical field to cells in four chambers, either cultured on the bottom of the culture-plate (2D) or embedded in hydrogel filled channels (3D). The set-up is also suitable for, live-cell-imaging. Validation tests show stable electrical fields and high cell viabilities inside the channel. Tumor spheroids of various diameters can be exposed to direct current electrical fields up to one week.
format	article
author	Kemkemer Ralf Naggay Benjamin K. Schmidt Tobias B. Ende Karen
author_facet	Kemkemer Ralf Naggay Benjamin K. Schmidt Tobias B. Ende Karen
author_sort	Kemkemer Ralf
title	Development of a multi-well-chip for studying 2D and 3D tumor cell migration and spheroid growth in electrical fields
title_short	Development of a multi-well-chip for studying 2D and 3D tumor cell migration and spheroid growth in electrical fields
title_full	Development of a multi-well-chip for studying 2D and 3D tumor cell migration and spheroid growth in electrical fields
title_fullStr	Development of a multi-well-chip for studying 2D and 3D tumor cell migration and spheroid growth in electrical fields
title_full_unstemmed	Development of a multi-well-chip for studying 2D and 3D tumor cell migration and spheroid growth in electrical fields
title_sort	development of a multi-well-chip for studying 2d and 3d tumor cell migration and spheroid growth in electrical fields
publisher	De Gruyter
publishDate	2020
url	https://doaj.org/article/dab6ee680b964b8092912ac2b3cc54bd
work_keys_str_mv	AT kemkemerralf developmentofamultiwellchipforstudying2dand3dtumorcellmigrationandspheroidgrowthinelectricalfields AT naggaybenjamink developmentofamultiwellchipforstudying2dand3dtumorcellmigrationandspheroidgrowthinelectricalfields AT schmidttobiasb developmentofamultiwellchipforstudying2dand3dtumorcellmigrationandspheroidgrowthinelectricalfields AT endekaren developmentofamultiwellchipforstudying2dand3dtumorcellmigrationandspheroidgrowthinelectricalfields
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Development of a multi-well-chip for studying 2D and 3D tumor cell migration and spheroid growth in electrical fields

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