Maturation of dendritic cells in vitro and immunological enhancement of mice in vivo by pachyman- and/or OVA-encapsulated poly(D,L-lactic acid) nanospheres
Sisi Zheng,1,* Tao Qin,2,* Yu Lu,3 Yifan Huang,2 Li Luo,1 Zhenguang Liu,1 Ruonan Bo,1 Yuanliang Hu,1 Jiaguo Liu,1 Deyun Wang1,2 1Institute of Traditional Chinese Veterinary Medicine, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, 2Fujian Key Laboratory of Traditional Chin...
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Dove Medical Press
2018
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oai:doaj.org-article:dac02810e55d422f869d8a0fc91ab0892021-12-02T05:09:48ZMaturation of dendritic cells in vitro and immunological enhancement of mice in vivo by pachyman- and/or OVA-encapsulated poly(D,L-lactic acid) nanospheres1178-2013https://doaj.org/article/dac02810e55d422f869d8a0fc91ab0892018-01-01T00:00:00Zhttps://www.dovepress.com/maturation-of-dendritic-cells-in-vitro-and-immunological-enhancement-o-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Sisi Zheng,1,* Tao Qin,2,* Yu Lu,3 Yifan Huang,2 Li Luo,1 Zhenguang Liu,1 Ruonan Bo,1 Yuanliang Hu,1 Jiaguo Liu,1 Deyun Wang1,2 1Institute of Traditional Chinese Veterinary Medicine, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, 2Fujian Key Laboratory of Traditional Chinese Veterinary Medicine and Animal, Fujian Agriculture and Forestry University, Fuzhou, 3National Veterinary Product Engineering Research Center, Jiangsu Academy of Agricultural Sciences, Nanjing, People’s Republic of China *These authors contributed equally to this work Background: Poly lactide (PLA) was proved in the last years to be good for use in sustained drug delivery and as carriers for vaccine antigens. In our previous research, pachyman (PHY)-encapsulated PLA (PHYP) nanospheres were synthesized and their function of controlling drug release was demonstrated.Purpose: In order to modify the fast drug-release rate of PHY when inoculated alone, the maturation of bone marrow dendritic cells (BMDCs) in vitro and their immunological enhancement in vivo were explored using PHYP nanospheres.Methods: The maturation and antigen uptake of BMDCs were evaluated, both alone and with formulated antigen PHYP nanospheres, ie, ovalbumin (OVA)-loaded PHYP nanospheres, as an antigen delivery system, to investigate antigen-specific humoral and cellular immune responses.Results: The results indicated that, when stimulated by PHYP, the BMDCs matured as a result of upregulated expression of co-stimulatory molecules; the mechanism was elucidated by tracing fluorescently labeled antigens in confocal laser scanning microscopy images and observing the uptake of nanospheres by transmission electron microscopy. It was further revealed that mice inoculated with OVA-PHYP had augmented antigen-specific IgG antibodies, increased cytokine secretion by splenocytes, increased splenocyte proliferation, and activation of cluster of differentiation (CD)4+ and CD8+ T cells in vivo. Elevated immune responses were produced by OVA-PHYP, possibly owing to the activation and maturation of dendritic cells (in draining lymph nodes).Conclusion: It was corroborated that PHY- and/or OVA-encapsulated PLA nanospheres elicited prominent antigen-presenting effects on BMDCs and heightened humoral and cellular immune responses compared with other formulations. Keywords: PHYP, bone marrow dendritic cell, antigen delivery system, immune responseZheng SSQin TLu YHuang YFLuo LLiu ZGBo RNHu YLLiu JGWang DYDove Medical PressarticlePHYPBone marrow dendritic cellAntigen delivery systemImmune responseMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 13, Pp 569-583 (2018) |
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PHYP Bone marrow dendritic cell Antigen delivery system Immune response Medicine (General) R5-920 |
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PHYP Bone marrow dendritic cell Antigen delivery system Immune response Medicine (General) R5-920 Zheng SS Qin T Lu Y Huang YF Luo L Liu ZG Bo RN Hu YL Liu JG Wang DY Maturation of dendritic cells in vitro and immunological enhancement of mice in vivo by pachyman- and/or OVA-encapsulated poly(D,L-lactic acid) nanospheres |
| description |
Sisi Zheng,1,* Tao Qin,2,* Yu Lu,3 Yifan Huang,2 Li Luo,1 Zhenguang Liu,1 Ruonan Bo,1 Yuanliang Hu,1 Jiaguo Liu,1 Deyun Wang1,2 1Institute of Traditional Chinese Veterinary Medicine, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, 2Fujian Key Laboratory of Traditional Chinese Veterinary Medicine and Animal, Fujian Agriculture and Forestry University, Fuzhou, 3National Veterinary Product Engineering Research Center, Jiangsu Academy of Agricultural Sciences, Nanjing, People’s Republic of China *These authors contributed equally to this work Background: Poly lactide (PLA) was proved in the last years to be good for use in sustained drug delivery and as carriers for vaccine antigens. In our previous research, pachyman (PHY)-encapsulated PLA (PHYP) nanospheres were synthesized and their function of controlling drug release was demonstrated.Purpose: In order to modify the fast drug-release rate of PHY when inoculated alone, the maturation of bone marrow dendritic cells (BMDCs) in vitro and their immunological enhancement in vivo were explored using PHYP nanospheres.Methods: The maturation and antigen uptake of BMDCs were evaluated, both alone and with formulated antigen PHYP nanospheres, ie, ovalbumin (OVA)-loaded PHYP nanospheres, as an antigen delivery system, to investigate antigen-specific humoral and cellular immune responses.Results: The results indicated that, when stimulated by PHYP, the BMDCs matured as a result of upregulated expression of co-stimulatory molecules; the mechanism was elucidated by tracing fluorescently labeled antigens in confocal laser scanning microscopy images and observing the uptake of nanospheres by transmission electron microscopy. It was further revealed that mice inoculated with OVA-PHYP had augmented antigen-specific IgG antibodies, increased cytokine secretion by splenocytes, increased splenocyte proliferation, and activation of cluster of differentiation (CD)4+ and CD8+ T cells in vivo. Elevated immune responses were produced by OVA-PHYP, possibly owing to the activation and maturation of dendritic cells (in draining lymph nodes).Conclusion: It was corroborated that PHY- and/or OVA-encapsulated PLA nanospheres elicited prominent antigen-presenting effects on BMDCs and heightened humoral and cellular immune responses compared with other formulations. Keywords: PHYP, bone marrow dendritic cell, antigen delivery system, immune response |
| format |
article |
| author |
Zheng SS Qin T Lu Y Huang YF Luo L Liu ZG Bo RN Hu YL Liu JG Wang DY |
| author_facet |
Zheng SS Qin T Lu Y Huang YF Luo L Liu ZG Bo RN Hu YL Liu JG Wang DY |
| author_sort |
Zheng SS |
| title |
Maturation of dendritic cells in vitro and immunological enhancement of mice in vivo by pachyman- and/or OVA-encapsulated poly(D,L-lactic acid) nanospheres |
| title_short |
Maturation of dendritic cells in vitro and immunological enhancement of mice in vivo by pachyman- and/or OVA-encapsulated poly(D,L-lactic acid) nanospheres |
| title_full |
Maturation of dendritic cells in vitro and immunological enhancement of mice in vivo by pachyman- and/or OVA-encapsulated poly(D,L-lactic acid) nanospheres |
| title_fullStr |
Maturation of dendritic cells in vitro and immunological enhancement of mice in vivo by pachyman- and/or OVA-encapsulated poly(D,L-lactic acid) nanospheres |
| title_full_unstemmed |
Maturation of dendritic cells in vitro and immunological enhancement of mice in vivo by pachyman- and/or OVA-encapsulated poly(D,L-lactic acid) nanospheres |
| title_sort |
maturation of dendritic cells in vitro and immunological enhancement of mice in vivo by pachyman- and/or ova-encapsulated poly(d,l-lactic acid) nanospheres |
| publisher |
Dove Medical Press |
| publishDate |
2018 |
| url |
https://doaj.org/article/dac02810e55d422f869d8a0fc91ab089 |
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