Genome-based discovery of a novel membrane-bound 1,6-dihydroxyphenazine prenyltransferase from a marine actinomycete.

Recently, novel prenylated derivatives of 1,6-dihydroxyphenazine have been isolated from the marine sponge-associated Streptomyces sp. SpC080624SC-11. Genome sequencing of this strain now revealed a gene cluster containing all genes necessary for the synthesis of the phenazine and the isoprenoid moi...

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Autores principales:	Philipp Zeyhle, Judith S Bauer, Jörn Kalinowski, Kazuo Shin-ya, Harald Gross, Lutz Heide
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2014
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/dac5357b2f74411290b2ece124dc21e8
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spelling	oai:doaj.org-article:dac5357b2f74411290b2ece124dc21e82021-11-18T08:17:25ZGenome-based discovery of a novel membrane-bound 1,6-dihydroxyphenazine prenyltransferase from a marine actinomycete.1932-620310.1371/journal.pone.0099122https://doaj.org/article/dac5357b2f74411290b2ece124dc21e82014-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24892559/?tool=EBIhttps://doaj.org/toc/1932-6203Recently, novel prenylated derivatives of 1,6-dihydroxyphenazine have been isolated from the marine sponge-associated Streptomyces sp. SpC080624SC-11. Genome sequencing of this strain now revealed a gene cluster containing all genes necessary for the synthesis of the phenazine and the isoprenoid moieties. Unexpectedly, however, the cluster did not contain a gene with similarity to previously investigated phenazine prenyltransferases, but instead a gene with modest similarity to the membrane-bound prenyltransferases of ubiquinone and menaquinone biosynthesis. Expression of this gene in E. coli and isolation of the membrane fraction proved that the encoded enzyme, Mpz10, catalyzes two successive prenylations of 1,6-dihydroxyphenazine. Mpz10 is the first example of a membrane-bound enzyme catalyzing the prenylation of a phenazine substrate, and one of few examples of membrane-bound enzymes involved in the prenylation of aromatic secondary metabolites in microorganisms.Philipp ZeyhleJudith S BauerJörn KalinowskiKazuo Shin-yaHarald GrossLutz HeidePublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 9, Iss 6, p e99122 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Philipp Zeyhle Judith S Bauer Jörn Kalinowski Kazuo Shin-ya Harald Gross Lutz Heide Genome-based discovery of a novel membrane-bound 1,6-dihydroxyphenazine prenyltransferase from a marine actinomycete.
description	Recently, novel prenylated derivatives of 1,6-dihydroxyphenazine have been isolated from the marine sponge-associated Streptomyces sp. SpC080624SC-11. Genome sequencing of this strain now revealed a gene cluster containing all genes necessary for the synthesis of the phenazine and the isoprenoid moieties. Unexpectedly, however, the cluster did not contain a gene with similarity to previously investigated phenazine prenyltransferases, but instead a gene with modest similarity to the membrane-bound prenyltransferases of ubiquinone and menaquinone biosynthesis. Expression of this gene in E. coli and isolation of the membrane fraction proved that the encoded enzyme, Mpz10, catalyzes two successive prenylations of 1,6-dihydroxyphenazine. Mpz10 is the first example of a membrane-bound enzyme catalyzing the prenylation of a phenazine substrate, and one of few examples of membrane-bound enzymes involved in the prenylation of aromatic secondary metabolites in microorganisms.
format	article
author	Philipp Zeyhle Judith S Bauer Jörn Kalinowski Kazuo Shin-ya Harald Gross Lutz Heide
author_facet	Philipp Zeyhle Judith S Bauer Jörn Kalinowski Kazuo Shin-ya Harald Gross Lutz Heide
author_sort	Philipp Zeyhle
title	Genome-based discovery of a novel membrane-bound 1,6-dihydroxyphenazine prenyltransferase from a marine actinomycete.
title_short	Genome-based discovery of a novel membrane-bound 1,6-dihydroxyphenazine prenyltransferase from a marine actinomycete.
title_full	Genome-based discovery of a novel membrane-bound 1,6-dihydroxyphenazine prenyltransferase from a marine actinomycete.
title_fullStr	Genome-based discovery of a novel membrane-bound 1,6-dihydroxyphenazine prenyltransferase from a marine actinomycete.
title_full_unstemmed	Genome-based discovery of a novel membrane-bound 1,6-dihydroxyphenazine prenyltransferase from a marine actinomycete.
title_sort	genome-based discovery of a novel membrane-bound 1,6-dihydroxyphenazine prenyltransferase from a marine actinomycete.
publisher	Public Library of Science (PLoS)
publishDate	2014
url	https://doaj.org/article/dac5357b2f74411290b2ece124dc21e8
work_keys_str_mv	AT philippzeyhle genomebaseddiscoveryofanovelmembranebound16dihydroxyphenazineprenyltransferasefromamarineactinomycete AT judithsbauer genomebaseddiscoveryofanovelmembranebound16dihydroxyphenazineprenyltransferasefromamarineactinomycete AT jornkalinowski genomebaseddiscoveryofanovelmembranebound16dihydroxyphenazineprenyltransferasefromamarineactinomycete AT kazuoshinya genomebaseddiscoveryofanovelmembranebound16dihydroxyphenazineprenyltransferasefromamarineactinomycete AT haraldgross genomebaseddiscoveryofanovelmembranebound16dihydroxyphenazineprenyltransferasefromamarineactinomycete AT lutzheide genomebaseddiscoveryofanovelmembranebound16dihydroxyphenazineprenyltransferasefromamarineactinomycete
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Genome-based discovery of a novel membrane-bound 1,6-dihydroxyphenazine prenyltransferase from a marine actinomycete.

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