Polycaprolactone scaffold engineered for sustained release of resveratrol: therapeutic enhancement in bone tissue engineering

Manjunath Srinivas Kamath,1 Shiek SSJ Ahmed,2 M Dhanasekaran,3 S Winkins Santosh11Department of Biotechnology, School of Bioengineering, SRM University, 2Department of Biotechnology, Chettinad Hospital and Research Institute, 3Department of Stem Cells, Life Line Rigid Hospital Pvt Ltd, Kilpauk, Tam...

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Autores principales:	Kamath MS, Ahmed SS, Dhanasekaran M, Winkins Santosh S
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2013
Materias:	Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/dac5c80b146349ea8377ddc50bec7e3b
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spelling	oai:doaj.org-article:dac5c80b146349ea8377ddc50bec7e3b2021-12-02T00:30:23ZPolycaprolactone scaffold engineered for sustained release of resveratrol: therapeutic enhancement in bone tissue engineering1178-2013https://doaj.org/article/dac5c80b146349ea8377ddc50bec7e3b2013-12-01T00:00:00Zhttp://www.dovepress.com/polycaprolactone-scaffold-engineered-for-sustained-release-of-resverat-a15368https://doaj.org/toc/1178-2013 Manjunath Srinivas Kamath,1 Shiek SSJ Ahmed,2 M Dhanasekaran,3 S Winkins Santosh11Department of Biotechnology, School of Bioengineering, SRM University, 2Department of Biotechnology, Chettinad Hospital and Research Institute, 3Department of Stem Cells, Life Line Rigid Hospital Pvt Ltd, Kilpauk, Tamil Nadu, IndiaAbstract: Biomaterials-based three-dimensional scaffolds are being extensively investigated in bone tissue engineering. A potential scaffold should be osteoconductive, osteoinductive, and osteogenic for enhanced bone formation. In this study, a three-dimensional porous polycaprolactone (PCL) scaffold was engineered for prolonged release of resveratrol. Resveratrol-loaded albumin nanoparticles (RNP) were synthesized and entrapped into a PCL scaffold to form PCL-RNP by a solvent casting and leaching method. An X-ray diffraction study of RNP and PCL-RNP showed that resveratrol underwent amorphization, which is highly desired in drug delivery. Furthermore, Fourier transform infrared spectroscopy indicates that resveratrol was not chemically modified during the entrapment process. Release of resveratrol from PCL-RNP was sustained, with a cumulative release of 64% at the end of day 12. The scaffold was evaluated for its bone-forming potential in vitro using human bone marrow-derived mesenchymal stem cells for 16 days. Alkaline phosphatase activity assayed on days 8 and 12 showed a significant increase in activity (1.6-fold and 1.4-fold, respectively) induced by PCL-RNP compared with the PCL scaffold (the positive control). Moreover, von Kossa staining for calcium deposits on day 16 showed increased mineralization in PCL-RNP. These results suggest PCL-RNP significantly improves mineralization due to its controlled and prolonged release of resveratrol, thereby increasing the therapeutic potential in bone tissue engineering.Keywords: therapeutic scaffolds, polycaprolactone scaffolds, bone tissue engineering, resveratrol, albumin nanoparticles, mesenchymal stem cellsKamath MSAhmed SSDhanasekaran MWinkins Santosh SDove Medical PressarticleMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2014, Iss Issue 1, Pp 183-195 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine (General) R5-920
spellingShingle	Medicine (General) R5-920 Kamath MS Ahmed SS Dhanasekaran M Winkins Santosh S Polycaprolactone scaffold engineered for sustained release of resveratrol: therapeutic enhancement in bone tissue engineering
description	Manjunath Srinivas Kamath,1 Shiek SSJ Ahmed,2 M Dhanasekaran,3 S Winkins Santosh11Department of Biotechnology, School of Bioengineering, SRM University, 2Department of Biotechnology, Chettinad Hospital and Research Institute, 3Department of Stem Cells, Life Line Rigid Hospital Pvt Ltd, Kilpauk, Tamil Nadu, IndiaAbstract: Biomaterials-based three-dimensional scaffolds are being extensively investigated in bone tissue engineering. A potential scaffold should be osteoconductive, osteoinductive, and osteogenic for enhanced bone formation. In this study, a three-dimensional porous polycaprolactone (PCL) scaffold was engineered for prolonged release of resveratrol. Resveratrol-loaded albumin nanoparticles (RNP) were synthesized and entrapped into a PCL scaffold to form PCL-RNP by a solvent casting and leaching method. An X-ray diffraction study of RNP and PCL-RNP showed that resveratrol underwent amorphization, which is highly desired in drug delivery. Furthermore, Fourier transform infrared spectroscopy indicates that resveratrol was not chemically modified during the entrapment process. Release of resveratrol from PCL-RNP was sustained, with a cumulative release of 64% at the end of day 12. The scaffold was evaluated for its bone-forming potential in vitro using human bone marrow-derived mesenchymal stem cells for 16 days. Alkaline phosphatase activity assayed on days 8 and 12 showed a significant increase in activity (1.6-fold and 1.4-fold, respectively) induced by PCL-RNP compared with the PCL scaffold (the positive control). Moreover, von Kossa staining for calcium deposits on day 16 showed increased mineralization in PCL-RNP. These results suggest PCL-RNP significantly improves mineralization due to its controlled and prolonged release of resveratrol, thereby increasing the therapeutic potential in bone tissue engineering.Keywords: therapeutic scaffolds, polycaprolactone scaffolds, bone tissue engineering, resveratrol, albumin nanoparticles, mesenchymal stem cells
format	article
author	Kamath MS Ahmed SS Dhanasekaran M Winkins Santosh S
author_facet	Kamath MS Ahmed SS Dhanasekaran M Winkins Santosh S
author_sort	Kamath MS
title	Polycaprolactone scaffold engineered for sustained release of resveratrol: therapeutic enhancement in bone tissue engineering
title_short	Polycaprolactone scaffold engineered for sustained release of resveratrol: therapeutic enhancement in bone tissue engineering
title_full	Polycaprolactone scaffold engineered for sustained release of resveratrol: therapeutic enhancement in bone tissue engineering
title_fullStr	Polycaprolactone scaffold engineered for sustained release of resveratrol: therapeutic enhancement in bone tissue engineering
title_full_unstemmed	Polycaprolactone scaffold engineered for sustained release of resveratrol: therapeutic enhancement in bone tissue engineering
title_sort	polycaprolactone scaffold engineered for sustained release of resveratrol: therapeutic enhancement in bone tissue engineering
publisher	Dove Medical Press
publishDate	2013
url	https://doaj.org/article/dac5c80b146349ea8377ddc50bec7e3b
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Polycaprolactone scaffold engineered for sustained release of resveratrol: therapeutic enhancement in bone tissue engineering

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