NOV/CCN3 Promotes Cell Migration and Invasion in Intrahepatic Cholangiocarcinoma via miR-92a-3p

Intrahepatic cholangiocarcinoma (ICC) is a common type of human cancer with a poor prognosis, and investigating the potential molecular mechanisms that can contribute to gene diagnosis and therapy. Herein, based on the recently concerned vertebrate-specific Cyr61/CTGF/NOV (CCN) gene family because o...

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Autores principales:	Tingming Liang, Lulu Shen, Yaya Ji, Lin Jia, Yuyang Dou, Li Guo
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	NOV/CCN3 miR-92a-3p cell migration invasion intrahepatic cholangiocarcinoma Genetics QH426-470
Acceso en línea:	https://doaj.org/article/dac86abe137641818d0a3ca0a38963ab
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spelling	oai:doaj.org-article:dac86abe137641818d0a3ca0a38963ab2021-11-25T17:40:21ZNOV/CCN3 Promotes Cell Migration and Invasion in Intrahepatic Cholangiocarcinoma via miR-92a-3p10.3390/genes121116592073-4425https://doaj.org/article/dac86abe137641818d0a3ca0a38963ab2021-10-01T00:00:00Zhttps://www.mdpi.com/2073-4425/12/11/1659https://doaj.org/toc/2073-4425Intrahepatic cholangiocarcinoma (ICC) is a common type of human cancer with a poor prognosis, and investigating the potential molecular mechanisms that can contribute to gene diagnosis and therapy. Herein, based on the recently concerned vertebrate-specific Cyr61/CTGF/NOV (CCN) gene family because of its important roles in diverse diseases, we obtained NOV/CCN3 to query for its potential roles in tumorigenesis via bioinformatics analysis. Experimental validations confirmed that both NOV mRNA and protein are up-regulated in two ICC cell lines, suggesting that it may promote cell migration and invasion by promoting EMT. To elucidate the detailed regulatory mechanism, miR-92a-3p is screened and identified as a negative regulatory small RNA targeting NOV, and further experimental validation demonstrates that miR-92a-3p contributes to NOV-mediated migration and invasion of ICC via the Notch signaling pathway. Our study reveals that NOV may be a potential target for diagnosing and treating ICC, which will provide experimental data and molecular theoretical foundation for cancer treatment, particularly for future precision medicine.Tingming LiangLulu ShenYaya JiLin JiaYuyang DouLi GuoMDPI AGarticleNOV/CCN3miR-92a-3pcell migrationinvasionintrahepatic cholangiocarcinomaGeneticsQH426-470ENGenes, Vol 12, Iss 1659, p 1659 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	NOV/CCN3 miR-92a-3p cell migration invasion intrahepatic cholangiocarcinoma Genetics QH426-470
spellingShingle	NOV/CCN3 miR-92a-3p cell migration invasion intrahepatic cholangiocarcinoma Genetics QH426-470 Tingming Liang Lulu Shen Yaya Ji Lin Jia Yuyang Dou Li Guo NOV/CCN3 Promotes Cell Migration and Invasion in Intrahepatic Cholangiocarcinoma via miR-92a-3p
description	Intrahepatic cholangiocarcinoma (ICC) is a common type of human cancer with a poor prognosis, and investigating the potential molecular mechanisms that can contribute to gene diagnosis and therapy. Herein, based on the recently concerned vertebrate-specific Cyr61/CTGF/NOV (CCN) gene family because of its important roles in diverse diseases, we obtained NOV/CCN3 to query for its potential roles in tumorigenesis via bioinformatics analysis. Experimental validations confirmed that both NOV mRNA and protein are up-regulated in two ICC cell lines, suggesting that it may promote cell migration and invasion by promoting EMT. To elucidate the detailed regulatory mechanism, miR-92a-3p is screened and identified as a negative regulatory small RNA targeting NOV, and further experimental validation demonstrates that miR-92a-3p contributes to NOV-mediated migration and invasion of ICC via the Notch signaling pathway. Our study reveals that NOV may be a potential target for diagnosing and treating ICC, which will provide experimental data and molecular theoretical foundation for cancer treatment, particularly for future precision medicine.
format	article
author	Tingming Liang Lulu Shen Yaya Ji Lin Jia Yuyang Dou Li Guo
author_facet	Tingming Liang Lulu Shen Yaya Ji Lin Jia Yuyang Dou Li Guo
author_sort	Tingming Liang
title	NOV/CCN3 Promotes Cell Migration and Invasion in Intrahepatic Cholangiocarcinoma via miR-92a-3p
title_short	NOV/CCN3 Promotes Cell Migration and Invasion in Intrahepatic Cholangiocarcinoma via miR-92a-3p
title_full	NOV/CCN3 Promotes Cell Migration and Invasion in Intrahepatic Cholangiocarcinoma via miR-92a-3p
title_fullStr	NOV/CCN3 Promotes Cell Migration and Invasion in Intrahepatic Cholangiocarcinoma via miR-92a-3p
title_full_unstemmed	NOV/CCN3 Promotes Cell Migration and Invasion in Intrahepatic Cholangiocarcinoma via miR-92a-3p
title_sort	nov/ccn3 promotes cell migration and invasion in intrahepatic cholangiocarcinoma via mir-92a-3p
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/dac86abe137641818d0a3ca0a38963ab
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NOV/CCN3 Promotes Cell Migration and Invasion in Intrahepatic Cholangiocarcinoma via miR-92a-3p

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