CREB Regulates Distinct Adaptive Transcriptional Programs in Astrocytes and Neurons

Abstract The cyclic AMP response element binding protein (CREB) is a primary hub of activity-driven genetic programs in neurons controlling plasticity, neurogenesis and survival. By contrast, the gene networks coordinated by CREB in astrocytes are unknown despite the fact that the astrocytic CREB is...

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Autores principales:	Luis Pardo, Luis Miguel Valor, Abel Eraso-Pichot, Angel Barco, Arantxa Golbano, Giles E. Hardingham, Roser Masgrau, Elena Galea
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/dacaf819c90c4629a7744a6f0b11f8b9
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spelling	oai:doaj.org-article:dacaf819c90c4629a7744a6f0b11f8b92021-12-02T16:06:25ZCREB Regulates Distinct Adaptive Transcriptional Programs in Astrocytes and Neurons10.1038/s41598-017-06231-x2045-2322https://doaj.org/article/dacaf819c90c4629a7744a6f0b11f8b92017-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-06231-xhttps://doaj.org/toc/2045-2322Abstract The cyclic AMP response element binding protein (CREB) is a primary hub of activity-driven genetic programs in neurons controlling plasticity, neurogenesis and survival. By contrast, the gene networks coordinated by CREB in astrocytes are unknown despite the fact that the astrocytic CREB is also activity-driven and neuroprotective. Herein we identified the transcriptional programs regulated by CREB in astrocytes as compared to neurons using, as study materials, transcriptome databases of astrocyte exposed to well-known activators of CREB-dependent transcription as well as publicly available transcriptomes of neuronal cultures. Functional CREB signatures were extracted from the transcriptomes using Gene Ontology, adult-brain gene lists generated by Translating Ribosome Affinity Purification (TRAP) and CREB-target gene repositories. We found minimal overlap between CREB signatures in astrocytes and neurons. In astrocytes, the top triad of functions regulated by CREB consists of ‘Gene expression’, ‘Mitochondria’, and ‘Signalling’, while in neurons it is ‘Neurotransmission’, ‘Signalling’ and ‘Gene expression’, the latter two being represented by different genes from those in astrocytes. The newly generated databases will provide a tool to explore novel means whereby CREB impinges on brain functions requiring adaptive, long-lasting changes by coordinating transcriptional cascades in astrocytes.Luis PardoLuis Miguel ValorAbel Eraso-PichotAngel BarcoArantxa GolbanoGiles E. HardinghamRoser MasgrauElena GaleaNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-14 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Luis Pardo Luis Miguel Valor Abel Eraso-Pichot Angel Barco Arantxa Golbano Giles E. Hardingham Roser Masgrau Elena Galea CREB Regulates Distinct Adaptive Transcriptional Programs in Astrocytes and Neurons
description	Abstract The cyclic AMP response element binding protein (CREB) is a primary hub of activity-driven genetic programs in neurons controlling plasticity, neurogenesis and survival. By contrast, the gene networks coordinated by CREB in astrocytes are unknown despite the fact that the astrocytic CREB is also activity-driven and neuroprotective. Herein we identified the transcriptional programs regulated by CREB in astrocytes as compared to neurons using, as study materials, transcriptome databases of astrocyte exposed to well-known activators of CREB-dependent transcription as well as publicly available transcriptomes of neuronal cultures. Functional CREB signatures were extracted from the transcriptomes using Gene Ontology, adult-brain gene lists generated by Translating Ribosome Affinity Purification (TRAP) and CREB-target gene repositories. We found minimal overlap between CREB signatures in astrocytes and neurons. In astrocytes, the top triad of functions regulated by CREB consists of ‘Gene expression’, ‘Mitochondria’, and ‘Signalling’, while in neurons it is ‘Neurotransmission’, ‘Signalling’ and ‘Gene expression’, the latter two being represented by different genes from those in astrocytes. The newly generated databases will provide a tool to explore novel means whereby CREB impinges on brain functions requiring adaptive, long-lasting changes by coordinating transcriptional cascades in astrocytes.
format	article
author	Luis Pardo Luis Miguel Valor Abel Eraso-Pichot Angel Barco Arantxa Golbano Giles E. Hardingham Roser Masgrau Elena Galea
author_facet	Luis Pardo Luis Miguel Valor Abel Eraso-Pichot Angel Barco Arantxa Golbano Giles E. Hardingham Roser Masgrau Elena Galea
author_sort	Luis Pardo
title	CREB Regulates Distinct Adaptive Transcriptional Programs in Astrocytes and Neurons
title_short	CREB Regulates Distinct Adaptive Transcriptional Programs in Astrocytes and Neurons
title_full	CREB Regulates Distinct Adaptive Transcriptional Programs in Astrocytes and Neurons
title_fullStr	CREB Regulates Distinct Adaptive Transcriptional Programs in Astrocytes and Neurons
title_full_unstemmed	CREB Regulates Distinct Adaptive Transcriptional Programs in Astrocytes and Neurons
title_sort	creb regulates distinct adaptive transcriptional programs in astrocytes and neurons
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/dacaf819c90c4629a7744a6f0b11f8b9
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CREB Regulates Distinct Adaptive Transcriptional Programs in Astrocytes and Neurons

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