Ectocytosis prevents accumulation of ciliary cargo in C. elegans sensory neurons

Cilia are sensory organelles protruding from cell surfaces. Release of extracellular vesicles (EVs) from cilia was previously observed in mammals, Chlamydomonas, and in male Caenorhabditis elegans. Using the EV marker TSP-6 (an ortholog of mammalian CD9) and other ciliary receptors, we show that EVs...

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Autores principales:	Adria Razzauti, Patrick Laurent
Formato:	article
Lenguaje:	EN
Publicado:	eLife Sciences Publications Ltd 2021
Materias:	extracellular vesicles cilia trafficking ectosomes neuron-glia interactions C. elegans sensory neurons Medicine R Science Q Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/dacb1906207141139cdac1863cb29756
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spelling	oai:doaj.org-article:dacb1906207141139cdac1863cb297562021-11-15T06:44:33ZEctocytosis prevents accumulation of ciliary cargo in C. elegans sensory neurons10.7554/eLife.676702050-084Xe67670https://doaj.org/article/dacb1906207141139cdac1863cb297562021-09-01T00:00:00Zhttps://elifesciences.org/articles/67670https://doaj.org/toc/2050-084XCilia are sensory organelles protruding from cell surfaces. Release of extracellular vesicles (EVs) from cilia was previously observed in mammals, Chlamydomonas, and in male Caenorhabditis elegans. Using the EV marker TSP-6 (an ortholog of mammalian CD9) and other ciliary receptors, we show that EVs are formed from ciliated sensory neurons in C. elegans hermaphrodites. Release of EVs is observed from two ciliary locations: the cilia tip and/or periciliary membrane compartment (PCMC). Outward budding of EVs from the cilia tip leads to their release into the environment. EVs’ budding from the PCMC is concomitantly phagocytosed by the associated glial cells. To maintain cilia composition, a tight regulation of cargo import and removal is achieved by the action of intra-flagellar transport (IFT). Unbalanced IFT due to cargo overexpression or mutations in the IFT machinery leads to local accumulation of ciliary proteins. Disposal of excess ciliary proteins via EVs reduces their local accumulation and exports them to the environment and/or to the glia associated to these ciliated neurons. We suggest that EV budding from cilia subcompartments acts as a safeguard mechanism to remove deleterious excess of ciliary material.Adria RazzautiPatrick LaurenteLife Sciences Publications Ltdarticleextracellular vesiclescilia traffickingectosomesneuron-glia interactionsC. eleganssensory neuronsMedicineRScienceQBiology (General)QH301-705.5ENeLife, Vol 10 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	extracellular vesicles cilia trafficking ectosomes neuron-glia interactions C. elegans sensory neurons Medicine R Science Q Biology (General) QH301-705.5
spellingShingle	extracellular vesicles cilia trafficking ectosomes neuron-glia interactions C. elegans sensory neurons Medicine R Science Q Biology (General) QH301-705.5 Adria Razzauti Patrick Laurent Ectocytosis prevents accumulation of ciliary cargo in C. elegans sensory neurons
description	Cilia are sensory organelles protruding from cell surfaces. Release of extracellular vesicles (EVs) from cilia was previously observed in mammals, Chlamydomonas, and in male Caenorhabditis elegans. Using the EV marker TSP-6 (an ortholog of mammalian CD9) and other ciliary receptors, we show that EVs are formed from ciliated sensory neurons in C. elegans hermaphrodites. Release of EVs is observed from two ciliary locations: the cilia tip and/or periciliary membrane compartment (PCMC). Outward budding of EVs from the cilia tip leads to their release into the environment. EVs’ budding from the PCMC is concomitantly phagocytosed by the associated glial cells. To maintain cilia composition, a tight regulation of cargo import and removal is achieved by the action of intra-flagellar transport (IFT). Unbalanced IFT due to cargo overexpression or mutations in the IFT machinery leads to local accumulation of ciliary proteins. Disposal of excess ciliary proteins via EVs reduces their local accumulation and exports them to the environment and/or to the glia associated to these ciliated neurons. We suggest that EV budding from cilia subcompartments acts as a safeguard mechanism to remove deleterious excess of ciliary material.
format	article
author	Adria Razzauti Patrick Laurent
author_facet	Adria Razzauti Patrick Laurent
author_sort	Adria Razzauti
title	Ectocytosis prevents accumulation of ciliary cargo in C. elegans sensory neurons
title_short	Ectocytosis prevents accumulation of ciliary cargo in C. elegans sensory neurons
title_full	Ectocytosis prevents accumulation of ciliary cargo in C. elegans sensory neurons
title_fullStr	Ectocytosis prevents accumulation of ciliary cargo in C. elegans sensory neurons
title_full_unstemmed	Ectocytosis prevents accumulation of ciliary cargo in C. elegans sensory neurons
title_sort	ectocytosis prevents accumulation of ciliary cargo in c. elegans sensory neurons
publisher	eLife Sciences Publications Ltd
publishDate	2021
url	https://doaj.org/article/dacb1906207141139cdac1863cb29756
work_keys_str_mv	AT adriarazzauti ectocytosispreventsaccumulationofciliarycargoinceleganssensoryneurons AT patricklaurent ectocytosispreventsaccumulationofciliarycargoinceleganssensoryneurons
_version_	1718428586355982336

Ectocytosis prevents accumulation of ciliary cargo in C. elegans sensory neurons

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