Depletion of <italic toggle="yes">Blautia</italic> Species in the Microbiota of Obese Children Relates to Intestinal Inflammation and Metabolic Phenotype Worsening

ABSTRACT Cross-sectional studies conducted with obese and control subjects have suggested associations between gut microbiota alterations and obesity, but the links with specific disease phenotypes and proofs of causality are still scarce. The present study aimed to profile the gut microbiota of lea...

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Autores principales:	Alfonso Benítez-Páez, Eva M. Gómez del Pugar, Inmaculada López-Almela, Ángela Moya-Pérez, Pilar Codoñer-Franch, Yolanda Sanz
Formato:	article
Lenguaje:	EN
Publicado:	American Society for Microbiology 2020
Materias:	Blautia luti Blautia wexlerae childhood obesity insulin resistance gut microbiota inflammation Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/dad36b24cc084b2da1dfa5682d96cbe6
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spelling	oai:doaj.org-article:dad36b24cc084b2da1dfa5682d96cbe62021-12-02T18:15:46ZDepletion of <italic toggle="yes">Blautia</italic> Species in the Microbiota of Obese Children Relates to Intestinal Inflammation and Metabolic Phenotype Worsening10.1128/mSystems.00857-192379-5077https://doaj.org/article/dad36b24cc084b2da1dfa5682d96cbe62020-04-01T00:00:00Zhttps://journals.asm.org/doi/10.1128/mSystems.00857-19https://doaj.org/toc/2379-5077ABSTRACT Cross-sectional studies conducted with obese and control subjects have suggested associations between gut microbiota alterations and obesity, but the links with specific disease phenotypes and proofs of causality are still scarce. The present study aimed to profile the gut microbiota of lean and obese children with and without insulin resistance to characterize associations with specific obesity-related complications and understand the role played in metabolic inflammation. Through massive sequencing of 16S rRNA gene amplicons and data analysis using a novel permutation approach, we have detected decreased incidence of Blautia species, especially Blautia luti and B. wexlerae, in the gut microbiota of obese children, which was even more pronounced in cases with both obesity and insulin resistance. There was also a parallel increase in proinflammatory cytokines and chemokines (gamma interferon [IFN-γ], tumor necrosis factor alpha [TNF-α], and monocyte chemoattractant protein 1 [MCP-1]) in feces of obese children compared to those of lean ones. B. luti and B. wexlerae were also shown to exert an anti-inflammatory effect in peripheral blood mononuclear cell cultures in vitro, compared to non-obesity-associated species. We suggest that the depletion of B. luti and B. wexlerae species in the gut ecosystem may occur in cases of obesity and contribute to metabolic inflammation leading to insulin resistance. IMPORTANCE Child obesity constitutes a risk factor for developing insulin resistance which, if sustained, could lead to more severe conditions like type 2 diabetes (T2D) in adulthood. Our study identified previously unknown species whose depletion (Blautia luti and Blautia wexlerae) is associated with insulin resistance in obese individuals. Our results also indicate that these bacterial species might help to reduce inflammation causally linked to obesity-related complications. Childhood is considered a window of opportunity to tackle obesity. These new findings provide, therefore, valuable information for the future design of microbiota-based strategies for the early prevention of obesity-related complications.Alfonso Benítez-PáezEva M. Gómez del PugarInmaculada López-AlmelaÁngela Moya-PérezPilar Codoñer-FranchYolanda SanzAmerican Society for MicrobiologyarticleBlautia lutiBlautia wexleraechildhood obesityinsulin resistancegut microbiotainflammationMicrobiologyQR1-502ENmSystems, Vol 5, Iss 2 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	Blautia luti Blautia wexlerae childhood obesity insulin resistance gut microbiota inflammation Microbiology QR1-502
spellingShingle	Blautia luti Blautia wexlerae childhood obesity insulin resistance gut microbiota inflammation Microbiology QR1-502 Alfonso Benítez-Páez Eva M. Gómez del Pugar Inmaculada López-Almela Ángela Moya-Pérez Pilar Codoñer-Franch Yolanda Sanz Depletion of <italic toggle="yes">Blautia</italic> Species in the Microbiota of Obese Children Relates to Intestinal Inflammation and Metabolic Phenotype Worsening
description	ABSTRACT Cross-sectional studies conducted with obese and control subjects have suggested associations between gut microbiota alterations and obesity, but the links with specific disease phenotypes and proofs of causality are still scarce. The present study aimed to profile the gut microbiota of lean and obese children with and without insulin resistance to characterize associations with specific obesity-related complications and understand the role played in metabolic inflammation. Through massive sequencing of 16S rRNA gene amplicons and data analysis using a novel permutation approach, we have detected decreased incidence of Blautia species, especially Blautia luti and B. wexlerae, in the gut microbiota of obese children, which was even more pronounced in cases with both obesity and insulin resistance. There was also a parallel increase in proinflammatory cytokines and chemokines (gamma interferon [IFN-γ], tumor necrosis factor alpha [TNF-α], and monocyte chemoattractant protein 1 [MCP-1]) in feces of obese children compared to those of lean ones. B. luti and B. wexlerae were also shown to exert an anti-inflammatory effect in peripheral blood mononuclear cell cultures in vitro, compared to non-obesity-associated species. We suggest that the depletion of B. luti and B. wexlerae species in the gut ecosystem may occur in cases of obesity and contribute to metabolic inflammation leading to insulin resistance. IMPORTANCE Child obesity constitutes a risk factor for developing insulin resistance which, if sustained, could lead to more severe conditions like type 2 diabetes (T2D) in adulthood. Our study identified previously unknown species whose depletion (Blautia luti and Blautia wexlerae) is associated with insulin resistance in obese individuals. Our results also indicate that these bacterial species might help to reduce inflammation causally linked to obesity-related complications. Childhood is considered a window of opportunity to tackle obesity. These new findings provide, therefore, valuable information for the future design of microbiota-based strategies for the early prevention of obesity-related complications.
format	article
author	Alfonso Benítez-Páez Eva M. Gómez del Pugar Inmaculada López-Almela Ángela Moya-Pérez Pilar Codoñer-Franch Yolanda Sanz
author_facet	Alfonso Benítez-Páez Eva M. Gómez del Pugar Inmaculada López-Almela Ángela Moya-Pérez Pilar Codoñer-Franch Yolanda Sanz
author_sort	Alfonso Benítez-Páez
title	Depletion of <italic toggle="yes">Blautia</italic> Species in the Microbiota of Obese Children Relates to Intestinal Inflammation and Metabolic Phenotype Worsening
title_short	Depletion of <italic toggle="yes">Blautia</italic> Species in the Microbiota of Obese Children Relates to Intestinal Inflammation and Metabolic Phenotype Worsening
title_full	Depletion of <italic toggle="yes">Blautia</italic> Species in the Microbiota of Obese Children Relates to Intestinal Inflammation and Metabolic Phenotype Worsening
title_fullStr	Depletion of <italic toggle="yes">Blautia</italic> Species in the Microbiota of Obese Children Relates to Intestinal Inflammation and Metabolic Phenotype Worsening
title_full_unstemmed	Depletion of <italic toggle="yes">Blautia</italic> Species in the Microbiota of Obese Children Relates to Intestinal Inflammation and Metabolic Phenotype Worsening
title_sort	depletion of <italic toggle="yes">blautia</italic> species in the microbiota of obese children relates to intestinal inflammation and metabolic phenotype worsening
publisher	American Society for Microbiology
publishDate	2020
url	https://doaj.org/article/dad36b24cc084b2da1dfa5682d96cbe6
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Depletion of <italic toggle="yes">Blautia</italic> Species in the Microbiota of Obese Children Relates to Intestinal Inflammation and Metabolic Phenotype Worsening

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