Embryonic Development of Avian Pineal Secretory Activity—A Lesson from the Goose Pineal Organs in Superfusion Culture

The embryonic ontogeny of pineal secretory activity in birds has been investigated almost exclusively in chickens. This study aimed to characterize this process in domestic geese. The pineal organs of embryos aged 18–28 days were incubated in superfusion culture under different light conditions for...

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Autores principales:	Maria Hanuszewska-Dominiak, Kamila Martyniuk, Bogdan Lewczuk
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	pineal organ melatonin indoles embryo ontogeny norepinephrine Organic chemistry QD241-441
Acceso en línea:	https://doaj.org/article/dae146ec85e44beb96dd4ad3685c7174
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spelling	oai:doaj.org-article:dae146ec85e44beb96dd4ad3685c71742021-11-11T18:21:52ZEmbryonic Development of Avian Pineal Secretory Activity—A Lesson from the Goose Pineal Organs in Superfusion Culture10.3390/molecules262163291420-3049https://doaj.org/article/dae146ec85e44beb96dd4ad3685c71742021-10-01T00:00:00Zhttps://www.mdpi.com/1420-3049/26/21/6329https://doaj.org/toc/1420-3049The embryonic ontogeny of pineal secretory activity in birds has been investigated almost exclusively in chickens. This study aimed to characterize this process in domestic geese. The pineal organs of embryos aged 18–28 days were incubated in superfusion culture under different light conditions for 4–5 days and treated with norepinephrine (NE). Melatonin (MLT) was measured by radioimmunoassay and other indoles by HPLC with fluorescence detection. Additionally, pineal organs were collected from embryos at 14–28 days of age and used to measure catecholamines by HPLC with electrochemical detection. MLT secretion increased with embryo age, most intensively between the 22nd and 24th days of life. The daily changes in MLT secretion under the 12 L:12D cycle occurred on the first day of culture, starting from an embryonic age of 24 days. MLT secretion was controlled by the light-dark cycle in all age groups studied. However, exposure to light during the scotophase did not alter the secretion of MLT. The endogenous oscillator expressed its activity in regulating MLT secretion in the pineal organs of embryos aged 24 days and older but could not generate a rhythm after one cycle. The rhythm of 5-hydroxytryptophan release during the first day of culture was found in the pineal organs of all embryos, while the rhythmic release of <i>N</i>-acetylserotonin and 5-methoxyindole acetic acid started at the age of 24 days. The proportion of released indoles changed with embryo age. NE caused a decrease in MLT secretion and provoked an increase in serotonin release. Incubation of the pineal organs induced the development of MLT secretory machinery and its diurnal rhythmicity. The pineal content of catecholamines increased prominently at the end of embryonic development.Maria Hanuszewska-DominiakKamila MartyniukBogdan LewczukMDPI AGarticlepineal organmelatoninindolesembryoontogenynorepinephrineOrganic chemistryQD241-441ENMolecules, Vol 26, Iss 6329, p 6329 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	pineal organ melatonin indoles embryo ontogeny norepinephrine Organic chemistry QD241-441
spellingShingle	pineal organ melatonin indoles embryo ontogeny norepinephrine Organic chemistry QD241-441 Maria Hanuszewska-Dominiak Kamila Martyniuk Bogdan Lewczuk Embryonic Development of Avian Pineal Secretory Activity—A Lesson from the Goose Pineal Organs in Superfusion Culture
description	The embryonic ontogeny of pineal secretory activity in birds has been investigated almost exclusively in chickens. This study aimed to characterize this process in domestic geese. The pineal organs of embryos aged 18–28 days were incubated in superfusion culture under different light conditions for 4–5 days and treated with norepinephrine (NE). Melatonin (MLT) was measured by radioimmunoassay and other indoles by HPLC with fluorescence detection. Additionally, pineal organs were collected from embryos at 14–28 days of age and used to measure catecholamines by HPLC with electrochemical detection. MLT secretion increased with embryo age, most intensively between the 22nd and 24th days of life. The daily changes in MLT secretion under the 12 L:12D cycle occurred on the first day of culture, starting from an embryonic age of 24 days. MLT secretion was controlled by the light-dark cycle in all age groups studied. However, exposure to light during the scotophase did not alter the secretion of MLT. The endogenous oscillator expressed its activity in regulating MLT secretion in the pineal organs of embryos aged 24 days and older but could not generate a rhythm after one cycle. The rhythm of 5-hydroxytryptophan release during the first day of culture was found in the pineal organs of all embryos, while the rhythmic release of <i>N</i>-acetylserotonin and 5-methoxyindole acetic acid started at the age of 24 days. The proportion of released indoles changed with embryo age. NE caused a decrease in MLT secretion and provoked an increase in serotonin release. Incubation of the pineal organs induced the development of MLT secretory machinery and its diurnal rhythmicity. The pineal content of catecholamines increased prominently at the end of embryonic development.
format	article
author	Maria Hanuszewska-Dominiak Kamila Martyniuk Bogdan Lewczuk
author_facet	Maria Hanuszewska-Dominiak Kamila Martyniuk Bogdan Lewczuk
author_sort	Maria Hanuszewska-Dominiak
title	Embryonic Development of Avian Pineal Secretory Activity—A Lesson from the Goose Pineal Organs in Superfusion Culture
title_short	Embryonic Development of Avian Pineal Secretory Activity—A Lesson from the Goose Pineal Organs in Superfusion Culture
title_full	Embryonic Development of Avian Pineal Secretory Activity—A Lesson from the Goose Pineal Organs in Superfusion Culture
title_fullStr	Embryonic Development of Avian Pineal Secretory Activity—A Lesson from the Goose Pineal Organs in Superfusion Culture
title_full_unstemmed	Embryonic Development of Avian Pineal Secretory Activity—A Lesson from the Goose Pineal Organs in Superfusion Culture
title_sort	embryonic development of avian pineal secretory activity—a lesson from the goose pineal organs in superfusion culture
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/dae146ec85e44beb96dd4ad3685c7174
work_keys_str_mv	AT mariahanuszewskadominiak embryonicdevelopmentofavianpinealsecretoryactivityalessonfromthegoosepinealorgansinsuperfusionculture AT kamilamartyniuk embryonicdevelopmentofavianpinealsecretoryactivityalessonfromthegoosepinealorgansinsuperfusionculture AT bogdanlewczuk embryonicdevelopmentofavianpinealsecretoryactivityalessonfromthegoosepinealorgansinsuperfusionculture
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Embryonic Development of Avian Pineal Secretory Activity—A Lesson from the Goose Pineal Organs in Superfusion Culture

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