Effects of titania nanotubes with or without bovine serum albumin loaded on human gingival fibroblasts

Xiangning Liu,1,* Xiaosong Zhou,2,* Shaobing Li,3 Renfa Lai,1 Zhiying Zhou,1 Ye Zhang,1 Lei Zhou3 1The First Affiliated Hospital of Jinan University, Guangzhou, 2Chemistry Science and Technology School, Zhanjiang Normal University, Zhanjiang, 3Guangdong Provincial Stomatological Hospital, Southern...

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Autores principales:	Liu X, Zhou X, Li S, Lai R, Zhou Z, Zhang Y, Zhou L
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2014
Materias:	Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/dae6187408974b26b047a4abc35a4599
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spelling	oai:doaj.org-article:dae6187408974b26b047a4abc35a45992021-12-02T02:04:17ZEffects of titania nanotubes with or without bovine serum albumin loaded on human gingival fibroblasts1178-2013https://doaj.org/article/dae6187408974b26b047a4abc35a45992014-03-01T00:00:00Zhttp://www.dovepress.com/effects-of-titania-nanotubes-with-or-without-bovine-serum-albumin-load-a16030https://doaj.org/toc/1178-2013 Xiangning Liu,1,* Xiaosong Zhou,2,* Shaobing Li,3 Renfa Lai,1 Zhiying Zhou,1 Ye Zhang,1 Lei Zhou3 1The First Affiliated Hospital of Jinan University, Guangzhou, 2Chemistry Science and Technology School, Zhanjiang Normal University, Zhanjiang, 3Guangdong Provincial Stomatological Hospital, Southern Medical University, Guangzhou, People's Republic of China *These authors contributed equally to this work Abstract: Modifying the surface of the transmucosal area is a key research area because this process positively affects the three functions of implants: attachment to soft tissue, inhibiting bacterial biofilm adhesion, and the preservation of the crestal bone. To exploit the potential of titania nanotube arrays (TNTs) with or without using bovine serum albumin (BSA) to modify the surface of a dental implant in contact with the transmucosal area, BSA was loaded into TNTs that were fabricated by anodizing Ti sheets; the physical characteristics of these arrays, including their morphology, chemical composition, surface roughness, contact angle, and surface free energy (SFE), were assessed. The effect of Ti surfaces with TNTs or TNTs-BSA on human gingival fibroblasts (HGFs) was determined by analyzing cell morphology, early adhesion, proliferation, type I collagen (COL-1) gene expression, and the extracellular secretion of COL-1. The results indicate that early HGF adhesion and spreading behavior is positively correlated with surface characteristics, including hydrophilicity, SFE, and surface roughness. Additionally, TNT surfaces not only promoted early HGF adhesion, but also promoted COL-1 secretion. BSA-loaded TNT surfaces promoted early HGF adhesion, while suppressing late proliferation and COL-1 secretion. Therefore, TNT-modified smooth surfaces are expected to be applicable for uses involving the transmucosal area. Further study is required to determine whether BSA-loaded TNT surfaces actually affect closed loop formation of connective tissue because BSA coating actions in vivo are very rapid. Keywords: titania nanotubes, bovine serum albumin, modified surface, transmucosal area, human gingival fibroblastLiu XZhou XLi SLai RZhou ZZhang YZhou LDove Medical PressarticleMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2014, Iss Issue 1, Pp 1185-1198 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine (General) R5-920
spellingShingle	Medicine (General) R5-920 Liu X Zhou X Li S Lai R Zhou Z Zhang Y Zhou L Effects of titania nanotubes with or without bovine serum albumin loaded on human gingival fibroblasts
description	Xiangning Liu,1,* Xiaosong Zhou,2,* Shaobing Li,3 Renfa Lai,1 Zhiying Zhou,1 Ye Zhang,1 Lei Zhou3 1The First Affiliated Hospital of Jinan University, Guangzhou, 2Chemistry Science and Technology School, Zhanjiang Normal University, Zhanjiang, 3Guangdong Provincial Stomatological Hospital, Southern Medical University, Guangzhou, People's Republic of China *These authors contributed equally to this work Abstract: Modifying the surface of the transmucosal area is a key research area because this process positively affects the three functions of implants: attachment to soft tissue, inhibiting bacterial biofilm adhesion, and the preservation of the crestal bone. To exploit the potential of titania nanotube arrays (TNTs) with or without using bovine serum albumin (BSA) to modify the surface of a dental implant in contact with the transmucosal area, BSA was loaded into TNTs that were fabricated by anodizing Ti sheets; the physical characteristics of these arrays, including their morphology, chemical composition, surface roughness, contact angle, and surface free energy (SFE), were assessed. The effect of Ti surfaces with TNTs or TNTs-BSA on human gingival fibroblasts (HGFs) was determined by analyzing cell morphology, early adhesion, proliferation, type I collagen (COL-1) gene expression, and the extracellular secretion of COL-1. The results indicate that early HGF adhesion and spreading behavior is positively correlated with surface characteristics, including hydrophilicity, SFE, and surface roughness. Additionally, TNT surfaces not only promoted early HGF adhesion, but also promoted COL-1 secretion. BSA-loaded TNT surfaces promoted early HGF adhesion, while suppressing late proliferation and COL-1 secretion. Therefore, TNT-modified smooth surfaces are expected to be applicable for uses involving the transmucosal area. Further study is required to determine whether BSA-loaded TNT surfaces actually affect closed loop formation of connective tissue because BSA coating actions in vivo are very rapid. Keywords: titania nanotubes, bovine serum albumin, modified surface, transmucosal area, human gingival fibroblast
format	article
author	Liu X Zhou X Li S Lai R Zhou Z Zhang Y Zhou L
author_facet	Liu X Zhou X Li S Lai R Zhou Z Zhang Y Zhou L
author_sort	Liu X
title	Effects of titania nanotubes with or without bovine serum albumin loaded on human gingival fibroblasts
title_short	Effects of titania nanotubes with or without bovine serum albumin loaded on human gingival fibroblasts
title_full	Effects of titania nanotubes with or without bovine serum albumin loaded on human gingival fibroblasts
title_fullStr	Effects of titania nanotubes with or without bovine serum albumin loaded on human gingival fibroblasts
title_full_unstemmed	Effects of titania nanotubes with or without bovine serum albumin loaded on human gingival fibroblasts
title_sort	effects of titania nanotubes with or without bovine serum albumin loaded on human gingival fibroblasts
publisher	Dove Medical Press
publishDate	2014
url	https://doaj.org/article/dae6187408974b26b047a4abc35a4599
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Effects of titania nanotubes with or without bovine serum albumin loaded on human gingival fibroblasts

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