Genome mapping coupled with CRISPR gene editing reveals a P450 gene confers avermectin resistance in the beet armyworm.

The evolution of insecticide resistance represents a global constraint to agricultural production. Because of the extreme genetic diversity found in insects and the large numbers of genes involved in insecticide detoxification, better tools are needed to quickly identify and validate the involvement...

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Autores principales:	Yayun Zuo, Yu Shi, Feng Zhang, Fang Guan, Jianpeng Zhang, René Feyereisen, Jeffrey A Fabrick, Yihua Yang, Yidong Wu
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2021
Materias:	Genetics QH426-470
Acceso en línea:	https://doaj.org/article/dae85ce50667431bb2945fd486292f62
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spelling	oai:doaj.org-article:dae85ce50667431bb2945fd486292f622021-12-02T20:02:56ZGenome mapping coupled with CRISPR gene editing reveals a P450 gene confers avermectin resistance in the beet armyworm.1553-73901553-740410.1371/journal.pgen.1009680https://doaj.org/article/dae85ce50667431bb2945fd486292f622021-07-01T00:00:00Zhttps://doi.org/10.1371/journal.pgen.1009680https://doaj.org/toc/1553-7390https://doaj.org/toc/1553-7404The evolution of insecticide resistance represents a global constraint to agricultural production. Because of the extreme genetic diversity found in insects and the large numbers of genes involved in insecticide detoxification, better tools are needed to quickly identify and validate the involvement of putative resistance genes for improved monitoring, management, and countering of field-evolved insecticide resistance. The avermectins, emamectin benzoate (EB) and abamectin are relatively new pesticides with reduced environmental risk that target a wide number of insect pests, including the beet armyworm, Spodoptera exigua, an important global pest of many crops. Unfortunately, field resistance to avermectins recently evolved in the beet armyworm, threatening the sustainable use of this class of insecticides. Here, we report a high-quality chromosome-level assembly of the beet armyworm genome and use bulked segregant analysis (BSA) to identify the locus of avermectin resistance, which mapped on 15-16 Mbp of chromosome 17. Knockout of the CYP9A186 gene that maps within this region by CRISPR/Cas9 gene editing fully restored EB susceptibility, implicating this gene in avermectin resistance. Heterologous expression and in vitro functional assays further confirm that a natural substitution (F116V) found in the substrate recognition site 1 (SRS1) of the CYP9A186 protein results in enhanced metabolism of EB and abamectin. Hence, the combined approach of coupling gene editing with BSA allows for the rapid identification of metabolic resistance genes responsible for insecticide resistance, which is critical for effective monitoring and adaptive management of insecticide resistance.Yayun ZuoYu ShiFeng ZhangFang GuanJianpeng ZhangRené FeyereisenJeffrey A FabrickYihua YangYidong WuPublic Library of Science (PLoS)articleGeneticsQH426-470ENPLoS Genetics, Vol 17, Iss 7, p e1009680 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Genetics QH426-470
spellingShingle	Genetics QH426-470 Yayun Zuo Yu Shi Feng Zhang Fang Guan Jianpeng Zhang René Feyereisen Jeffrey A Fabrick Yihua Yang Yidong Wu Genome mapping coupled with CRISPR gene editing reveals a P450 gene confers avermectin resistance in the beet armyworm.
description	The evolution of insecticide resistance represents a global constraint to agricultural production. Because of the extreme genetic diversity found in insects and the large numbers of genes involved in insecticide detoxification, better tools are needed to quickly identify and validate the involvement of putative resistance genes for improved monitoring, management, and countering of field-evolved insecticide resistance. The avermectins, emamectin benzoate (EB) and abamectin are relatively new pesticides with reduced environmental risk that target a wide number of insect pests, including the beet armyworm, Spodoptera exigua, an important global pest of many crops. Unfortunately, field resistance to avermectins recently evolved in the beet armyworm, threatening the sustainable use of this class of insecticides. Here, we report a high-quality chromosome-level assembly of the beet armyworm genome and use bulked segregant analysis (BSA) to identify the locus of avermectin resistance, which mapped on 15-16 Mbp of chromosome 17. Knockout of the CYP9A186 gene that maps within this region by CRISPR/Cas9 gene editing fully restored EB susceptibility, implicating this gene in avermectin resistance. Heterologous expression and in vitro functional assays further confirm that a natural substitution (F116V) found in the substrate recognition site 1 (SRS1) of the CYP9A186 protein results in enhanced metabolism of EB and abamectin. Hence, the combined approach of coupling gene editing with BSA allows for the rapid identification of metabolic resistance genes responsible for insecticide resistance, which is critical for effective monitoring and adaptive management of insecticide resistance.
format	article
author	Yayun Zuo Yu Shi Feng Zhang Fang Guan Jianpeng Zhang René Feyereisen Jeffrey A Fabrick Yihua Yang Yidong Wu
author_facet	Yayun Zuo Yu Shi Feng Zhang Fang Guan Jianpeng Zhang René Feyereisen Jeffrey A Fabrick Yihua Yang Yidong Wu
author_sort	Yayun Zuo
title	Genome mapping coupled with CRISPR gene editing reveals a P450 gene confers avermectin resistance in the beet armyworm.
title_short	Genome mapping coupled with CRISPR gene editing reveals a P450 gene confers avermectin resistance in the beet armyworm.
title_full	Genome mapping coupled with CRISPR gene editing reveals a P450 gene confers avermectin resistance in the beet armyworm.
title_fullStr	Genome mapping coupled with CRISPR gene editing reveals a P450 gene confers avermectin resistance in the beet armyworm.
title_full_unstemmed	Genome mapping coupled with CRISPR gene editing reveals a P450 gene confers avermectin resistance in the beet armyworm.
title_sort	genome mapping coupled with crispr gene editing reveals a p450 gene confers avermectin resistance in the beet armyworm.
publisher	Public Library of Science (PLoS)
publishDate	2021
url	https://doaj.org/article/dae85ce50667431bb2945fd486292f62
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Genome mapping coupled with CRISPR gene editing reveals a P450 gene confers avermectin resistance in the beet armyworm.

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