Isolation, characterization and efficacy of lytic bacteriophages against pathogenic Escherichia coli from hospital liquid waste

Abstract. Lingga R, Budiarti S, Rusmana I, Wahyu AT. 2020. Isolation, characterization and efficacy of lytic bacteriophages against pathogenic Escherichia coli from hospital liquid waste. Biodiversitas 21: 3234-3241. Escherichia coli is known as a pathogenic contaminant bacteria in hospital wastewat...

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Autores principales:	Rahmad Lingga, Sri Budiarti, Iman Rusmana, Aris Tri Wahyudi
Formato:	article
Lenguaje:	EN
Publicado:	MBI & UNS Solo 2020
Materias:	escherichia coli, lytic bacteriophage, hospital liquid waste, antibiotic-resistant Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/dafe774dd54849e8a256f9c2a410cc35
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spelling	oai:doaj.org-article:dafe774dd54849e8a256f9c2a410cc352021-11-22T00:40:51ZIsolation, characterization and efficacy of lytic bacteriophages against pathogenic Escherichia coli from hospital liquid waste1412-033X2085-472210.13057/biodiv/d210745https://doaj.org/article/dafe774dd54849e8a256f9c2a410cc352020-06-01T00:00:00Zhttps://smujo.id/biodiv/article/view/5732https://doaj.org/toc/1412-033Xhttps://doaj.org/toc/2085-4722Abstract. Lingga R, Budiarti S, Rusmana I, Wahyu AT. 2020. Isolation, characterization and efficacy of lytic bacteriophages against pathogenic Escherichia coli from hospital liquid waste. Biodiversitas 21: 3234-3241. Escherichia coli is known as a pathogenic contaminant bacteria in hospital wastewater hazardous to humans and the environment. Concerns about the emergence of chlorine- and antibiotic-resistant bacteria increase the urgency to find an alternative strategy to control pathogenic bacteria in hospital wastewater. One of the alternatives is using lytic bacteriophage. This study aimed to isolate, characterize, and examine the efficacy of lytic bacteriophage against pathogenic Escherichia coli from hospital wastewater. It isolated, characterized (plaque morphology, host range, virion electron micrograph, and sensitivity to temperature, pH, and chlorine treatments), and tested the efficacy of lytic bacteriophages in controlling pathogenic E. coli isolated from hospital wastewater. Five phages were successfully obtained, all of which had clear plaques (lytic phage character). Based on host range assay, most of the phages could lyse all tested E. coli strains but not for other species. Electron micrograph photography revealed that the phages belonged to Myoviridae. The phages showed stability in high temperature, broad-ranged pH, and high concentrations of chlorine treatments. Assay on phages efficacy suggested that the phages are capable of significantly reducing the E. coli population both in sterilized and non-sterilized wastewater. The combination of phage treatment and chlorine was more effective than single phage treatment. The efficacy test revealed that phage application in wastewater had the best result seen from cocktail treatment and a combination of phage treatment and chlorine. These results suggested that the phage can be a potential candidate for disinfection purposes.Rahmad LinggaSri BudiartiIman RusmanaAris Tri WahyudiMBI & UNS Soloarticleescherichia coli, lytic bacteriophage, hospital liquid waste, antibiotic-resistantBiology (General)QH301-705.5ENBiodiversitas, Vol 21, Iss 7 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	escherichia coli, lytic bacteriophage, hospital liquid waste, antibiotic-resistant Biology (General) QH301-705.5
spellingShingle	escherichia coli, lytic bacteriophage, hospital liquid waste, antibiotic-resistant Biology (General) QH301-705.5 Rahmad Lingga Sri Budiarti Iman Rusmana Aris Tri Wahyudi Isolation, characterization and efficacy of lytic bacteriophages against pathogenic Escherichia coli from hospital liquid waste
description	Abstract. Lingga R, Budiarti S, Rusmana I, Wahyu AT. 2020. Isolation, characterization and efficacy of lytic bacteriophages against pathogenic Escherichia coli from hospital liquid waste. Biodiversitas 21: 3234-3241. Escherichia coli is known as a pathogenic contaminant bacteria in hospital wastewater hazardous to humans and the environment. Concerns about the emergence of chlorine- and antibiotic-resistant bacteria increase the urgency to find an alternative strategy to control pathogenic bacteria in hospital wastewater. One of the alternatives is using lytic bacteriophage. This study aimed to isolate, characterize, and examine the efficacy of lytic bacteriophage against pathogenic Escherichia coli from hospital wastewater. It isolated, characterized (plaque morphology, host range, virion electron micrograph, and sensitivity to temperature, pH, and chlorine treatments), and tested the efficacy of lytic bacteriophages in controlling pathogenic E. coli isolated from hospital wastewater. Five phages were successfully obtained, all of which had clear plaques (lytic phage character). Based on host range assay, most of the phages could lyse all tested E. coli strains but not for other species. Electron micrograph photography revealed that the phages belonged to Myoviridae. The phages showed stability in high temperature, broad-ranged pH, and high concentrations of chlorine treatments. Assay on phages efficacy suggested that the phages are capable of significantly reducing the E. coli population both in sterilized and non-sterilized wastewater. The combination of phage treatment and chlorine was more effective than single phage treatment. The efficacy test revealed that phage application in wastewater had the best result seen from cocktail treatment and a combination of phage treatment and chlorine. These results suggested that the phage can be a potential candidate for disinfection purposes.
format	article
author	Rahmad Lingga Sri Budiarti Iman Rusmana Aris Tri Wahyudi
author_facet	Rahmad Lingga Sri Budiarti Iman Rusmana Aris Tri Wahyudi
author_sort	Rahmad Lingga
title	Isolation, characterization and efficacy of lytic bacteriophages against pathogenic Escherichia coli from hospital liquid waste
title_short	Isolation, characterization and efficacy of lytic bacteriophages against pathogenic Escherichia coli from hospital liquid waste
title_full	Isolation, characterization and efficacy of lytic bacteriophages against pathogenic Escherichia coli from hospital liquid waste
title_fullStr	Isolation, characterization and efficacy of lytic bacteriophages against pathogenic Escherichia coli from hospital liquid waste
title_full_unstemmed	Isolation, characterization and efficacy of lytic bacteriophages against pathogenic Escherichia coli from hospital liquid waste
title_sort	isolation, characterization and efficacy of lytic bacteriophages against pathogenic escherichia coli from hospital liquid waste
publisher	MBI & UNS Solo
publishDate	2020
url	https://doaj.org/article/dafe774dd54849e8a256f9c2a410cc35
work_keys_str_mv	AT rahmadlingga isolationcharacterizationandefficacyoflyticbacteriophagesagainstpathogenicescherichiacolifromhospitalliquidwaste AT sribudiarti isolationcharacterizationandefficacyoflyticbacteriophagesagainstpathogenicescherichiacolifromhospitalliquidwaste AT imanrusmana isolationcharacterizationandefficacyoflyticbacteriophagesagainstpathogenicescherichiacolifromhospitalliquidwaste AT aristriwahyudi isolationcharacterizationandefficacyoflyticbacteriophagesagainstpathogenicescherichiacolifromhospitalliquidwaste
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Isolation, characterization and efficacy of lytic bacteriophages against pathogenic Escherichia coli from hospital liquid waste

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