The First Eight Mitogenomes of Leaf-Mining <i>Dactylispa</i> Beetles (Coleoptera: Chrysomelidae: Cassidinae) Shed New Light on Subgenus Relationships
The taxonomic classification of <i>Dactylispa</i>, a large genus of leaf-mining beetles, is problematic because it is currently based on morphology alone. Here, the first eight mitochondrial genomes of <i>Dactylispa</i> species, which were used to construct the first molecula...
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| Auteurs principaux: | , , , , , , |
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| Format: | article |
| Langue: | EN |
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MDPI AG
2021
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| Accès en ligne: | https://doaj.org/article/daff14cc5b0447639fb6bcd6fb1817b0 |
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| Résumé: | The taxonomic classification of <i>Dactylispa</i>, a large genus of leaf-mining beetles, is problematic because it is currently based on morphology alone. Here, the first eight mitochondrial genomes of <i>Dactylispa</i> species, which were used to construct the first molecular phylogenies of this genus, are reported. The lengths of the eight mitogenomes range from 17,189 bp to 20,363 bp. All of the mitochondrial genomes include 13 protein-coding genes (PCGs), 22 transfer RNA genes (tRNAs), 2 ribosomal RNA genes (rRNAs), and 1 A + T-rich region. According to the nonsynonymous/synonymous mutation ratio (Ka/Ks) of all PCGs, the highest and the lowest evolutionary rates were found for atp8 and cox1, respectively, which is a common phenomenon among animals. According to relative synonymous codon usage, UUA(L) has the highest frequency. With two Gonophorini species as the outgroup, mitogenome-based phylogenetic trees of the eight <i>Dactylispa</i> species were constructed using maximum likelihood (ML) and Bayesian inference (BI) methods based on the PCGs, tRNAs, and rRNAs. Two DNA-based phylogenomic inferences and one protein-based phylogenomic inference support the delimitation of the subgenera <i>Dactylispa s. str</i>. and <i>Platypriella</i> as proposed in the system of Chen et al. (1986). However, the subgenus <i>Triplispa</i> is not recovered as monophyletic. The placement of <i>Triplispa</i> species requires further verification and testing with more species. We also found that both adult body shape and host plant relationship might explain the subgeneric relationships among <i>Dactylispa</i> beetles to a certain degree. |
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