Preparation of bioactive interferon alpha– loaded polysaccharide nanoparticles using a new approach of temperature-induced water phase/water-phase emulsion
Guang Liu,1,* Dong Xu,2,* Mier Jiang,1 Weien Yuan21Department of Vascular Surgery, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China; 2School of Pharmacy, Shanghai Jiao Tong University, Shanghai, China*These authors contributed equally to...
Guardado en:
| Autores principales: | , , , |
|---|---|
| Formato: | article |
| Lenguaje: | EN |
| Publicado: |
Dove Medical Press
2012
|
| Materias: | |
| Acceso en línea: | https://doaj.org/article/db09aadd26af49ffba4a31a906672e97 |
| Etiquetas: |
Agregar Etiqueta
Sin Etiquetas, Sea el primero en etiquetar este registro!
|
| id |
oai:doaj.org-article:db09aadd26af49ffba4a31a906672e97 |
|---|---|
| record_format |
dspace |
| spelling |
oai:doaj.org-article:db09aadd26af49ffba4a31a906672e972021-12-02T01:08:06ZPreparation of bioactive interferon alpha– loaded polysaccharide nanoparticles using a new approach of temperature-induced water phase/water-phase emulsion1176-91141178-2013https://doaj.org/article/db09aadd26af49ffba4a31a906672e972012-09-01T00:00:00Zhttp://www.dovepress.com/preparation-of-bioactive-interferon-alphandash-loaded-polysaccharide-n-a10935https://doaj.org/toc/1176-9114https://doaj.org/toc/1178-2013Guang Liu,1,* Dong Xu,2,* Mier Jiang,1 Weien Yuan21Department of Vascular Surgery, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China; 2School of Pharmacy, Shanghai Jiao Tong University, Shanghai, China*These authors contributed equally to this workAbstract: The aim of this study was to develop a temperature-induced polyethylene glycol (PEG) water phase/polysaccharide water-phase emulsion approach for preparing interferon alpha-2b (IFNα-2b)-loaded polysaccharide nanoparticles. IFNα-2b was first added to a mixture of an aqueous solution of PEG and polysaccharide. The mixture solution was stirred in a magnetic stirrer at a rate of 2000 rpm for 45 seconds at 0°C ± 0.5°C. The solution was then prefrozen at different temperatures. The polysaccharide and IFNα-2b partitioned in the polysaccharide phase were preferentially separated out as the dispersed phase from the mixture solution during the prefreezing process. Then the prefrozen sample was freeze-dried to powder form. In order to remove the PEG, the powder was washed with dichloromethane. Once IFNα-2b was loaded into the polysaccharide nanoparticles, these nanoparticles could gain resistance to vapor–water and water–oil interfaces to protect IFNα-2b. The antiviral activity of the polysaccharide nanoparticles in vitro was highly preserved (above 97%), while the antiviral activity of IFNα-2b–loaded polysaccharide nanoparticles using the control water-in-oil-in-water method was only 71%. The antiviral activity of the IFNα-2b from blood samples was also determined on the basis of the activity to inhibit the cytopathic effects of the Sindbis virus on Follicular Lymphoma cells (FL). The antiviral activity in vivo was also highly preserved (above 97%). These polysaccharide nanoparticles could be processed to different formulations according to clinical requirements.Keywords: activity of interferon alpha-2b, interferon alpha-2b, stability of interferon alpha-2b, dextran, nanoparticlesYuan WJiang MXu DLiu GDove Medical PressarticleMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2012, Iss default, Pp 4841-4848 (2012) |
| institution |
DOAJ |
| collection |
DOAJ |
| language |
EN |
| topic |
Medicine (General) R5-920 |
| spellingShingle |
Medicine (General) R5-920 Yuan W Jiang M Xu D Liu G Preparation of bioactive interferon alpha– loaded polysaccharide nanoparticles using a new approach of temperature-induced water phase/water-phase emulsion |
| description |
Guang Liu,1,* Dong Xu,2,* Mier Jiang,1 Weien Yuan21Department of Vascular Surgery, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China; 2School of Pharmacy, Shanghai Jiao Tong University, Shanghai, China*These authors contributed equally to this workAbstract: The aim of this study was to develop a temperature-induced polyethylene glycol (PEG) water phase/polysaccharide water-phase emulsion approach for preparing interferon alpha-2b (IFNα-2b)-loaded polysaccharide nanoparticles. IFNα-2b was first added to a mixture of an aqueous solution of PEG and polysaccharide. The mixture solution was stirred in a magnetic stirrer at a rate of 2000 rpm for 45 seconds at 0°C ± 0.5°C. The solution was then prefrozen at different temperatures. The polysaccharide and IFNα-2b partitioned in the polysaccharide phase were preferentially separated out as the dispersed phase from the mixture solution during the prefreezing process. Then the prefrozen sample was freeze-dried to powder form. In order to remove the PEG, the powder was washed with dichloromethane. Once IFNα-2b was loaded into the polysaccharide nanoparticles, these nanoparticles could gain resistance to vapor–water and water–oil interfaces to protect IFNα-2b. The antiviral activity of the polysaccharide nanoparticles in vitro was highly preserved (above 97%), while the antiviral activity of IFNα-2b–loaded polysaccharide nanoparticles using the control water-in-oil-in-water method was only 71%. The antiviral activity of the IFNα-2b from blood samples was also determined on the basis of the activity to inhibit the cytopathic effects of the Sindbis virus on Follicular Lymphoma cells (FL). The antiviral activity in vivo was also highly preserved (above 97%). These polysaccharide nanoparticles could be processed to different formulations according to clinical requirements.Keywords: activity of interferon alpha-2b, interferon alpha-2b, stability of interferon alpha-2b, dextran, nanoparticles |
| format |
article |
| author |
Yuan W Jiang M Xu D Liu G |
| author_facet |
Yuan W Jiang M Xu D Liu G |
| author_sort |
Yuan W |
| title |
Preparation of bioactive interferon alpha– loaded polysaccharide nanoparticles using a new approach of temperature-induced water phase/water-phase emulsion |
| title_short |
Preparation of bioactive interferon alpha– loaded polysaccharide nanoparticles using a new approach of temperature-induced water phase/water-phase emulsion |
| title_full |
Preparation of bioactive interferon alpha– loaded polysaccharide nanoparticles using a new approach of temperature-induced water phase/water-phase emulsion |
| title_fullStr |
Preparation of bioactive interferon alpha– loaded polysaccharide nanoparticles using a new approach of temperature-induced water phase/water-phase emulsion |
| title_full_unstemmed |
Preparation of bioactive interferon alpha– loaded polysaccharide nanoparticles using a new approach of temperature-induced water phase/water-phase emulsion |
| title_sort |
preparation of bioactive interferon alpha– loaded polysaccharide nanoparticles using a new approach of temperature-induced water phase/water-phase emulsion |
| publisher |
Dove Medical Press |
| publishDate |
2012 |
| url |
https://doaj.org/article/db09aadd26af49ffba4a31a906672e97 |
| work_keys_str_mv |
AT yuanw preparationofbioactiveinterferonalphaampndashloadedpolysaccharidenanoparticlesusinganewapproachoftemperatureinducedwaterphasewaterphaseemulsion AT jiangm preparationofbioactiveinterferonalphaampndashloadedpolysaccharidenanoparticlesusinganewapproachoftemperatureinducedwaterphasewaterphaseemulsion AT xud preparationofbioactiveinterferonalphaampndashloadedpolysaccharidenanoparticlesusinganewapproachoftemperatureinducedwaterphasewaterphaseemulsion AT liug preparationofbioactiveinterferonalphaampndashloadedpolysaccharidenanoparticlesusinganewapproachoftemperatureinducedwaterphasewaterphaseemulsion |
| _version_ |
1718403286246096896 |