Microendoscopic calcium imaging of the primary visual cortex of behaving macaques

Abstract In vivo calcium imaging with genetically encoded indicators has recently been applied to macaque brains to monitor neural activities from a large population of cells simultaneously. Microendoscopic calcium imaging combined with implantable gradient index lenses captures neural activities fr...

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Autores principales:	Mineki Oguchi, Jiang Jiasen, Toshihide W. Yoshioka, Yasuhiro R. Tanaka, Kenichi Inoue, Masahiko Takada, Takefumi Kikusui, Kensaku Nomoto, Masamichi Sakagami
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/db12884e1456463b87510553d1fd445e
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spelling	oai:doaj.org-article:db12884e1456463b87510553d1fd445e2021-12-02T18:53:09ZMicroendoscopic calcium imaging of the primary visual cortex of behaving macaques10.1038/s41598-021-96532-z2045-2322https://doaj.org/article/db12884e1456463b87510553d1fd445e2021-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-96532-zhttps://doaj.org/toc/2045-2322Abstract In vivo calcium imaging with genetically encoded indicators has recently been applied to macaque brains to monitor neural activities from a large population of cells simultaneously. Microendoscopic calcium imaging combined with implantable gradient index lenses captures neural activities from deep brain areas with a compact and convenient setup; however, this has been limited to rodents and marmosets. Here, we developed miniature fluorescent microscopy to image neural activities from the primary visual cortex of behaving macaques. We found tens of clear fluorescent signals from three of the six brain hemispheres. A subset of these neurons showed clear retinotopy and orientation tuning. Moreover, we successfully decoded the stimulus orientation and tracked the cells across days. These results indicate that microendoscopic calcium imaging is feasible and reasonable for investigating neural circuits in the macaque brain by monitoring fluorescent signals from a large number of neurons.Mineki OguchiJiang JiasenToshihide W. YoshiokaYasuhiro R. TanakaKenichi InoueMasahiko TakadaTakefumi KikusuiKensaku NomotoMasamichi SakagamiNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-15 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Mineki Oguchi Jiang Jiasen Toshihide W. Yoshioka Yasuhiro R. Tanaka Kenichi Inoue Masahiko Takada Takefumi Kikusui Kensaku Nomoto Masamichi Sakagami Microendoscopic calcium imaging of the primary visual cortex of behaving macaques
description	Abstract In vivo calcium imaging with genetically encoded indicators has recently been applied to macaque brains to monitor neural activities from a large population of cells simultaneously. Microendoscopic calcium imaging combined with implantable gradient index lenses captures neural activities from deep brain areas with a compact and convenient setup; however, this has been limited to rodents and marmosets. Here, we developed miniature fluorescent microscopy to image neural activities from the primary visual cortex of behaving macaques. We found tens of clear fluorescent signals from three of the six brain hemispheres. A subset of these neurons showed clear retinotopy and orientation tuning. Moreover, we successfully decoded the stimulus orientation and tracked the cells across days. These results indicate that microendoscopic calcium imaging is feasible and reasonable for investigating neural circuits in the macaque brain by monitoring fluorescent signals from a large number of neurons.
format	article
author	Mineki Oguchi Jiang Jiasen Toshihide W. Yoshioka Yasuhiro R. Tanaka Kenichi Inoue Masahiko Takada Takefumi Kikusui Kensaku Nomoto Masamichi Sakagami
author_facet	Mineki Oguchi Jiang Jiasen Toshihide W. Yoshioka Yasuhiro R. Tanaka Kenichi Inoue Masahiko Takada Takefumi Kikusui Kensaku Nomoto Masamichi Sakagami
author_sort	Mineki Oguchi
title	Microendoscopic calcium imaging of the primary visual cortex of behaving macaques
title_short	Microendoscopic calcium imaging of the primary visual cortex of behaving macaques
title_full	Microendoscopic calcium imaging of the primary visual cortex of behaving macaques
title_fullStr	Microendoscopic calcium imaging of the primary visual cortex of behaving macaques
title_full_unstemmed	Microendoscopic calcium imaging of the primary visual cortex of behaving macaques
title_sort	microendoscopic calcium imaging of the primary visual cortex of behaving macaques
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/db12884e1456463b87510553d1fd445e
work_keys_str_mv	AT minekioguchi microendoscopiccalciumimagingoftheprimaryvisualcortexofbehavingmacaques AT jiangjiasen microendoscopiccalciumimagingoftheprimaryvisualcortexofbehavingmacaques AT toshihidewyoshioka microendoscopiccalciumimagingoftheprimaryvisualcortexofbehavingmacaques AT yasuhirortanaka microendoscopiccalciumimagingoftheprimaryvisualcortexofbehavingmacaques AT kenichiinoue microendoscopiccalciumimagingoftheprimaryvisualcortexofbehavingmacaques AT masahikotakada microendoscopiccalciumimagingoftheprimaryvisualcortexofbehavingmacaques AT takefumikikusui microendoscopiccalciumimagingoftheprimaryvisualcortexofbehavingmacaques AT kensakunomoto microendoscopiccalciumimagingoftheprimaryvisualcortexofbehavingmacaques AT masamichisakagami microendoscopiccalciumimagingoftheprimaryvisualcortexofbehavingmacaques
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Microendoscopic calcium imaging of the primary visual cortex of behaving macaques

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