Intergenerational Patterns of DNA Methylation in <i>Procambarus clarkii</i> Following Exposure to Genotoxicants: A Conjugation in Past Simple or Past Continuous?

Epigenome is susceptible to modulation by environmental pressures—namely, through alterations in global DNA methylation, impacting the organism condition and, ultimately, reverberating on the phenotype of the subsequent generations. Hence, an intergenerational study was conducted, aiming to clarify...

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Autores principales:	Raquel Marçal, Lola Llorente, Oscar Herrero, Rosario Planelló, Sofia Guilherme, Mário Pacheco
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	crustacean epigenetics methylome intergenerational genotoxic pesticides Chemical technology TP1-1185
Acceso en línea:	https://doaj.org/article/db1d295432f9423e807422d05eb31ba0
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spelling	oai:doaj.org-article:db1d295432f9423e807422d05eb31ba02021-11-25T19:07:57ZIntergenerational Patterns of DNA Methylation in <i>Procambarus clarkii</i> Following Exposure to Genotoxicants: A Conjugation in Past Simple or Past Continuous?10.3390/toxics91102712305-6304https://doaj.org/article/db1d295432f9423e807422d05eb31ba02021-10-01T00:00:00Zhttps://www.mdpi.com/2305-6304/9/11/271https://doaj.org/toc/2305-6304Epigenome is susceptible to modulation by environmental pressures—namely, through alterations in global DNA methylation, impacting the organism condition and, ultimately, reverberating on the phenotype of the subsequent generations. Hence, an intergenerational study was conducted, aiming to clarify the influence of genotoxicants on global DNA methylation of the crayfish <i>Procambarus clarkii</i>. Two subsequent generations were exposed to the herbicide penoxsulam (Px; 23 µg·L<sup>−1</sup>) and to the genotoxicant model ethyl methanesulfonate (EMS; 5 mg·L<sup>−1</sup>). Px did not induce changes in DNA methylation of adult crayfish (F<sub>0</sub>). However, the hypomethylation occurring in unexposed F<sub>1</sub> juveniles demonstrated that the history of exposure per se can modulate epigenome. In F<sub>1</sub> descendants of the Px-exposed group, methylome (hypermethylated) was more affected in males than in females. EMS-induced hypomethylation in adult females (F<sub>0</sub>), also showed gender specificity. In addition, hypomethylation was also observed in the unexposed F<sub>1</sub> crayfish, indicating an intergenerational epigenetic effect. The modulatory role of past exposure to penoxsulam or to EMS also showed a dependency on the crayfish developmental stage. Overall, this research revealed that indirect experiences (events occurring in a predecessor generation) can have an impact even greater than direct experiences (present events) on the epigenetic dynamics.Raquel MarçalLola LlorenteOscar HerreroRosario PlanellóSofia GuilhermeMário PachecoMDPI AGarticlecrustaceanepigeneticsmethylomeintergenerationalgenotoxicpesticidesChemical technologyTP1-1185ENToxics, Vol 9, Iss 271, p 271 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	crustacean epigenetics methylome intergenerational genotoxic pesticides Chemical technology TP1-1185
spellingShingle	crustacean epigenetics methylome intergenerational genotoxic pesticides Chemical technology TP1-1185 Raquel Marçal Lola Llorente Oscar Herrero Rosario Planelló Sofia Guilherme Mário Pacheco Intergenerational Patterns of DNA Methylation in <i>Procambarus clarkii</i> Following Exposure to Genotoxicants: A Conjugation in Past Simple or Past Continuous?
description	Epigenome is susceptible to modulation by environmental pressures—namely, through alterations in global DNA methylation, impacting the organism condition and, ultimately, reverberating on the phenotype of the subsequent generations. Hence, an intergenerational study was conducted, aiming to clarify the influence of genotoxicants on global DNA methylation of the crayfish <i>Procambarus clarkii</i>. Two subsequent generations were exposed to the herbicide penoxsulam (Px; 23 µg·L<sup>−1</sup>) and to the genotoxicant model ethyl methanesulfonate (EMS; 5 mg·L<sup>−1</sup>). Px did not induce changes in DNA methylation of adult crayfish (F<sub>0</sub>). However, the hypomethylation occurring in unexposed F<sub>1</sub> juveniles demonstrated that the history of exposure per se can modulate epigenome. In F<sub>1</sub> descendants of the Px-exposed group, methylome (hypermethylated) was more affected in males than in females. EMS-induced hypomethylation in adult females (F<sub>0</sub>), also showed gender specificity. In addition, hypomethylation was also observed in the unexposed F<sub>1</sub> crayfish, indicating an intergenerational epigenetic effect. The modulatory role of past exposure to penoxsulam or to EMS also showed a dependency on the crayfish developmental stage. Overall, this research revealed that indirect experiences (events occurring in a predecessor generation) can have an impact even greater than direct experiences (present events) on the epigenetic dynamics.
format	article
author	Raquel Marçal Lola Llorente Oscar Herrero Rosario Planelló Sofia Guilherme Mário Pacheco
author_facet	Raquel Marçal Lola Llorente Oscar Herrero Rosario Planelló Sofia Guilherme Mário Pacheco
author_sort	Raquel Marçal
title	Intergenerational Patterns of DNA Methylation in <i>Procambarus clarkii</i> Following Exposure to Genotoxicants: A Conjugation in Past Simple or Past Continuous?
title_short	Intergenerational Patterns of DNA Methylation in <i>Procambarus clarkii</i> Following Exposure to Genotoxicants: A Conjugation in Past Simple or Past Continuous?
title_full	Intergenerational Patterns of DNA Methylation in <i>Procambarus clarkii</i> Following Exposure to Genotoxicants: A Conjugation in Past Simple or Past Continuous?
title_fullStr	Intergenerational Patterns of DNA Methylation in <i>Procambarus clarkii</i> Following Exposure to Genotoxicants: A Conjugation in Past Simple or Past Continuous?
title_full_unstemmed	Intergenerational Patterns of DNA Methylation in <i>Procambarus clarkii</i> Following Exposure to Genotoxicants: A Conjugation in Past Simple or Past Continuous?
title_sort	intergenerational patterns of dna methylation in <i>procambarus clarkii</i> following exposure to genotoxicants: a conjugation in past simple or past continuous?
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/db1d295432f9423e807422d05eb31ba0
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Intergenerational Patterns of DNA Methylation in <i>Procambarus clarkii</i> Following Exposure to Genotoxicants: A Conjugation in Past Simple or Past Continuous?

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