Acylcarnitine profile in Alaskan sled dogs during submaximal multiday exercise points out metabolic flexibility and liver role in energy metabolism.

Alaskan sled dogs develop a particular metabolic strategy during multiday submaximal exercise, allowing them to switch from intra-muscular to extra-muscular energy substrates thus postponing fatigue. Specifically, a progressively increasing stimulus for hepatic glycogenolysis and gluconeogenesis pro...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Irene Tosi, Tatiana Art, François Boemer, Dominique-Marie Votion, Michael S Davis
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/db21bba279414de98ece3ceaaa39186f
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:db21bba279414de98ece3ceaaa39186f
record_format	dspace
spelling	oai:doaj.org-article:db21bba279414de98ece3ceaaa39186f2021-12-02T20:18:16ZAcylcarnitine profile in Alaskan sled dogs during submaximal multiday exercise points out metabolic flexibility and liver role in energy metabolism.1932-620310.1371/journal.pone.0256009https://doaj.org/article/db21bba279414de98ece3ceaaa39186f2021-01-01T00:00:00Zhttps://doi.org/10.1371/journal.pone.0256009https://doaj.org/toc/1932-6203Alaskan sled dogs develop a particular metabolic strategy during multiday submaximal exercise, allowing them to switch from intra-muscular to extra-muscular energy substrates thus postponing fatigue. Specifically, a progressively increasing stimulus for hepatic glycogenolysis and gluconeogenesis provides glucose for both fueling exercise and replenishing the depleted muscle glycogen. Moreover, recent studies have shown that with continuation of exercise sled dogs increase their insulin-sensitivity and their capacity to transport and oxidize glucose and carbohydrates rather than oxidizing fatty acids. Carnitine and acylcarnitines (AC) play an essential role as metabolic regulators in both fat and glucose metabolism; they serve as biomarkers in different species in both physiologic and pathologic conditions. We assessed the effect of multiday exercise in conditioned sled dogs on plasma short (SC), medium (MC) and long (LC) chain AC by tandem mass spectrometry (MS/MS). Our results show chain-specific modification of AC profiles during the exercise challenge: LCACs maintained a steady increase throughout exercise, some SCACs increased during the last phase of exercise and acetylcarnitine (C2) initially increased before decreasing during the later phase of exercise. We speculated that SCACs kinetics could reflect an increased protein catabolism and C2 pattern could reflect its hepatic uptake for energy-generating purposes to sustain gluconeogenesis. LCACs may be exported by muscle to avoid their accumulation to preserve glucose oxidation and insulin-sensitivity or they could be distributed by liver as energy substrates. These findings, although representing a "snapshot" of blood as a crossing point between different organs, shed further light on sled dogs metabolism that is liver-centric and more carbohydrate-dependent than fat-dependent and during prolonged submaximal exercise.Irene TosiTatiana ArtFrançois BoemerDominique-Marie VotionMichael S DavisPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 16, Iss 8, p e0256009 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Irene Tosi Tatiana Art François Boemer Dominique-Marie Votion Michael S Davis Acylcarnitine profile in Alaskan sled dogs during submaximal multiday exercise points out metabolic flexibility and liver role in energy metabolism.
description	Alaskan sled dogs develop a particular metabolic strategy during multiday submaximal exercise, allowing them to switch from intra-muscular to extra-muscular energy substrates thus postponing fatigue. Specifically, a progressively increasing stimulus for hepatic glycogenolysis and gluconeogenesis provides glucose for both fueling exercise and replenishing the depleted muscle glycogen. Moreover, recent studies have shown that with continuation of exercise sled dogs increase their insulin-sensitivity and their capacity to transport and oxidize glucose and carbohydrates rather than oxidizing fatty acids. Carnitine and acylcarnitines (AC) play an essential role as metabolic regulators in both fat and glucose metabolism; they serve as biomarkers in different species in both physiologic and pathologic conditions. We assessed the effect of multiday exercise in conditioned sled dogs on plasma short (SC), medium (MC) and long (LC) chain AC by tandem mass spectrometry (MS/MS). Our results show chain-specific modification of AC profiles during the exercise challenge: LCACs maintained a steady increase throughout exercise, some SCACs increased during the last phase of exercise and acetylcarnitine (C2) initially increased before decreasing during the later phase of exercise. We speculated that SCACs kinetics could reflect an increased protein catabolism and C2 pattern could reflect its hepatic uptake for energy-generating purposes to sustain gluconeogenesis. LCACs may be exported by muscle to avoid their accumulation to preserve glucose oxidation and insulin-sensitivity or they could be distributed by liver as energy substrates. These findings, although representing a "snapshot" of blood as a crossing point between different organs, shed further light on sled dogs metabolism that is liver-centric and more carbohydrate-dependent than fat-dependent and during prolonged submaximal exercise.
format	article
author	Irene Tosi Tatiana Art François Boemer Dominique-Marie Votion Michael S Davis
author_facet	Irene Tosi Tatiana Art François Boemer Dominique-Marie Votion Michael S Davis
author_sort	Irene Tosi
title	Acylcarnitine profile in Alaskan sled dogs during submaximal multiday exercise points out metabolic flexibility and liver role in energy metabolism.
title_short	Acylcarnitine profile in Alaskan sled dogs during submaximal multiday exercise points out metabolic flexibility and liver role in energy metabolism.
title_full	Acylcarnitine profile in Alaskan sled dogs during submaximal multiday exercise points out metabolic flexibility and liver role in energy metabolism.
title_fullStr	Acylcarnitine profile in Alaskan sled dogs during submaximal multiday exercise points out metabolic flexibility and liver role in energy metabolism.
title_full_unstemmed	Acylcarnitine profile in Alaskan sled dogs during submaximal multiday exercise points out metabolic flexibility and liver role in energy metabolism.
title_sort	acylcarnitine profile in alaskan sled dogs during submaximal multiday exercise points out metabolic flexibility and liver role in energy metabolism.
publisher	Public Library of Science (PLoS)
publishDate	2021
url	https://doaj.org/article/db21bba279414de98ece3ceaaa39186f
work_keys_str_mv	AT irenetosi acylcarnitineprofileinalaskansleddogsduringsubmaximalmultidayexercisepointsoutmetabolicflexibilityandliverroleinenergymetabolism AT tatianaart acylcarnitineprofileinalaskansleddogsduringsubmaximalmultidayexercisepointsoutmetabolicflexibilityandliverroleinenergymetabolism AT francoisboemer acylcarnitineprofileinalaskansleddogsduringsubmaximalmultidayexercisepointsoutmetabolicflexibilityandliverroleinenergymetabolism AT dominiquemarievotion acylcarnitineprofileinalaskansleddogsduringsubmaximalmultidayexercisepointsoutmetabolicflexibilityandliverroleinenergymetabolism AT michaelsdavis acylcarnitineprofileinalaskansleddogsduringsubmaximalmultidayexercisepointsoutmetabolicflexibilityandliverroleinenergymetabolism
_version_	1718374316250234880

Acylcarnitine profile in Alaskan sled dogs during submaximal multiday exercise points out metabolic flexibility and liver role in energy metabolism.

Ejemplares similares